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Molecular Surveillance for Vector-Borne Bacteria in Rodents and Tree Shrews of Peninsular Malaysia Oil Palm Plantations
Many human clinical cases attributed to vector-borne pathogens are underreported in Malaysia, especially in rural localities where healthcare infrastructures are lacking. Here, 217 small mammals, consisting of rodents and tree shrews, were trapped in oil palm plantations in the Peninsular Malaysia s...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965954/ https://www.ncbi.nlm.nih.gov/pubmed/36828490 http://dx.doi.org/10.3390/tropicalmed8020074 |
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author | Mohd-Azami, Siti Nurul Izzah Loong, Shih Keng Khoo, Jing Jing Husin, Nurul Aini Lim, Fang Shiang Mahfodz, Nur Hidayana Ishak, Siti Nabilah Mohd-Taib, Farah Shafawati Makepeace, Benjamin L. AbuBakar, Sazaly |
author_facet | Mohd-Azami, Siti Nurul Izzah Loong, Shih Keng Khoo, Jing Jing Husin, Nurul Aini Lim, Fang Shiang Mahfodz, Nur Hidayana Ishak, Siti Nabilah Mohd-Taib, Farah Shafawati Makepeace, Benjamin L. AbuBakar, Sazaly |
author_sort | Mohd-Azami, Siti Nurul Izzah |
collection | PubMed |
description | Many human clinical cases attributed to vector-borne pathogens are underreported in Malaysia, especially in rural localities where healthcare infrastructures are lacking. Here, 217 small mammals, consisting of rodents and tree shrews, were trapped in oil palm plantations in the Peninsular Malaysia states of Johor and Perak. Species identification was performed using morphological and DNA barcoding analyses, and 203 small mammals were included in the detection of selected vector-borne bacteria. The DNA extracted from the spleens was examined for Orientia tsutsugamushi, Borrelia spp., Bartonella spp. and Rickettsia spp. using established PCR assays. The small mammals collected in this study included Rattus tanezumi R3 mitotype (n = 113), Rattus argentiventer (n = 24), Rattus tiomanicus (n = 22), Rattus exulans (n = 17), Rattus tanezumi sensu stricto (n = 1) and Tupaia glis (n = 40). Orientia tsutsugamushi, Borrelia spp. and Bartonella phoceensis were detected in the small mammals with the respective detection rates of 12.3%, 5.9% and 4.9%. Rickettsia spp., however, was not detected. This study encountered the presence of both Lyme disease and relapsing fever-related borreliae in small mammals collected from the oil palm plantation study sites. All three microorganisms (Orientia tsutsugamushi, Borrelia spp. and Bartonella phoceensis) were detected in the R. tanezumi R3 mitotype, suggesting that the species is a competent host for multiple microorganisms. Further investigations are warranted to elucidate the relationships between the ectoparasites, the small mammals and the respective pathogens. |
format | Online Article Text |
id | pubmed-9965954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99659542023-02-26 Molecular Surveillance for Vector-Borne Bacteria in Rodents and Tree Shrews of Peninsular Malaysia Oil Palm Plantations Mohd-Azami, Siti Nurul Izzah Loong, Shih Keng Khoo, Jing Jing Husin, Nurul Aini Lim, Fang Shiang Mahfodz, Nur Hidayana Ishak, Siti Nabilah Mohd-Taib, Farah Shafawati Makepeace, Benjamin L. AbuBakar, Sazaly Trop Med Infect Dis Article Many human clinical cases attributed to vector-borne pathogens are underreported in Malaysia, especially in rural localities where healthcare infrastructures are lacking. Here, 217 small mammals, consisting of rodents and tree shrews, were trapped in oil palm plantations in the Peninsular Malaysia states of Johor and Perak. Species identification was performed using morphological and DNA barcoding analyses, and 203 small mammals were included in the detection of selected vector-borne bacteria. The DNA extracted from the spleens was examined for Orientia tsutsugamushi, Borrelia spp., Bartonella spp. and Rickettsia spp. using established PCR assays. The small mammals collected in this study included Rattus tanezumi R3 mitotype (n = 113), Rattus argentiventer (n = 24), Rattus tiomanicus (n = 22), Rattus exulans (n = 17), Rattus tanezumi sensu stricto (n = 1) and Tupaia glis (n = 40). Orientia tsutsugamushi, Borrelia spp. and Bartonella phoceensis were detected in the small mammals with the respective detection rates of 12.3%, 5.9% and 4.9%. Rickettsia spp., however, was not detected. This study encountered the presence of both Lyme disease and relapsing fever-related borreliae in small mammals collected from the oil palm plantation study sites. All three microorganisms (Orientia tsutsugamushi, Borrelia spp. and Bartonella phoceensis) were detected in the R. tanezumi R3 mitotype, suggesting that the species is a competent host for multiple microorganisms. Further investigations are warranted to elucidate the relationships between the ectoparasites, the small mammals and the respective pathogens. MDPI 2023-01-19 /pmc/articles/PMC9965954/ /pubmed/36828490 http://dx.doi.org/10.3390/tropicalmed8020074 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mohd-Azami, Siti Nurul Izzah Loong, Shih Keng Khoo, Jing Jing Husin, Nurul Aini Lim, Fang Shiang Mahfodz, Nur Hidayana Ishak, Siti Nabilah Mohd-Taib, Farah Shafawati Makepeace, Benjamin L. AbuBakar, Sazaly Molecular Surveillance for Vector-Borne Bacteria in Rodents and Tree Shrews of Peninsular Malaysia Oil Palm Plantations |
title | Molecular Surveillance for Vector-Borne Bacteria in Rodents and Tree Shrews of Peninsular Malaysia Oil Palm Plantations |
title_full | Molecular Surveillance for Vector-Borne Bacteria in Rodents and Tree Shrews of Peninsular Malaysia Oil Palm Plantations |
title_fullStr | Molecular Surveillance for Vector-Borne Bacteria in Rodents and Tree Shrews of Peninsular Malaysia Oil Palm Plantations |
title_full_unstemmed | Molecular Surveillance for Vector-Borne Bacteria in Rodents and Tree Shrews of Peninsular Malaysia Oil Palm Plantations |
title_short | Molecular Surveillance for Vector-Borne Bacteria in Rodents and Tree Shrews of Peninsular Malaysia Oil Palm Plantations |
title_sort | molecular surveillance for vector-borne bacteria in rodents and tree shrews of peninsular malaysia oil palm plantations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9965954/ https://www.ncbi.nlm.nih.gov/pubmed/36828490 http://dx.doi.org/10.3390/tropicalmed8020074 |
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