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Identification of Potential Antiviral Hops Compounds against Chikungunya Virus
Chikungunya virus (CHIKV) is an arthropod-borne virus that belongs to the genus Alphavirus (family Togaviridae). CHIKV causes chikungunya fever, which is mostly characterized by fever, arthralgia and, sometimes, a maculopapular rash. The bioactive constituents of hops (Humulus lupulus, Cannabaceae),...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966109/ https://www.ncbi.nlm.nih.gov/pubmed/36834745 http://dx.doi.org/10.3390/ijms24043333 |
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author | Mandova, Tsvetelina Saivish, Marielena Vogel La Serra, Leonardo Nogueira, Mauricio Lacerda Da Costa, Fernando Batista |
author_facet | Mandova, Tsvetelina Saivish, Marielena Vogel La Serra, Leonardo Nogueira, Mauricio Lacerda Da Costa, Fernando Batista |
author_sort | Mandova, Tsvetelina |
collection | PubMed |
description | Chikungunya virus (CHIKV) is an arthropod-borne virus that belongs to the genus Alphavirus (family Togaviridae). CHIKV causes chikungunya fever, which is mostly characterized by fever, arthralgia and, sometimes, a maculopapular rash. The bioactive constituents of hops (Humulus lupulus, Cannabaceae), mainly acylphloroglucinols, known as well as α- and β-acids, exerted distinct activity against CHIKV, without showing cytotoxicity. For fast and efficient isolation and identification of such bioactive constituents, a silica-free countercurrent separation method was applied. The antiviral activity was determined by plaque reduction test and was visually confirmed by a cell-based immunofluorescence assay. All hops compounds demonstrated a promising post-treatment viral inhibition, except the fraction of acylphloroglucinols, in mixture. β-acids fraction of 125 µg/mL expressed the strongest virucidal activity (EC(50) = 15.21 µg/mL), in a drug-addition experiment on Vero cells. Hypothesis for mechanism of action were proposed for acylphloroglucinols based on their lipophilicity and chemical structure. Therefore, inhibition of some steps of the protein kinase C (PKC) transduction cascades was also discussed. |
format | Online Article Text |
id | pubmed-9966109 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99661092023-02-26 Identification of Potential Antiviral Hops Compounds against Chikungunya Virus Mandova, Tsvetelina Saivish, Marielena Vogel La Serra, Leonardo Nogueira, Mauricio Lacerda Da Costa, Fernando Batista Int J Mol Sci Article Chikungunya virus (CHIKV) is an arthropod-borne virus that belongs to the genus Alphavirus (family Togaviridae). CHIKV causes chikungunya fever, which is mostly characterized by fever, arthralgia and, sometimes, a maculopapular rash. The bioactive constituents of hops (Humulus lupulus, Cannabaceae), mainly acylphloroglucinols, known as well as α- and β-acids, exerted distinct activity against CHIKV, without showing cytotoxicity. For fast and efficient isolation and identification of such bioactive constituents, a silica-free countercurrent separation method was applied. The antiviral activity was determined by plaque reduction test and was visually confirmed by a cell-based immunofluorescence assay. All hops compounds demonstrated a promising post-treatment viral inhibition, except the fraction of acylphloroglucinols, in mixture. β-acids fraction of 125 µg/mL expressed the strongest virucidal activity (EC(50) = 15.21 µg/mL), in a drug-addition experiment on Vero cells. Hypothesis for mechanism of action were proposed for acylphloroglucinols based on their lipophilicity and chemical structure. Therefore, inhibition of some steps of the protein kinase C (PKC) transduction cascades was also discussed. MDPI 2023-02-07 /pmc/articles/PMC9966109/ /pubmed/36834745 http://dx.doi.org/10.3390/ijms24043333 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mandova, Tsvetelina Saivish, Marielena Vogel La Serra, Leonardo Nogueira, Mauricio Lacerda Da Costa, Fernando Batista Identification of Potential Antiviral Hops Compounds against Chikungunya Virus |
title | Identification of Potential Antiviral Hops Compounds against Chikungunya Virus |
title_full | Identification of Potential Antiviral Hops Compounds against Chikungunya Virus |
title_fullStr | Identification of Potential Antiviral Hops Compounds against Chikungunya Virus |
title_full_unstemmed | Identification of Potential Antiviral Hops Compounds against Chikungunya Virus |
title_short | Identification of Potential Antiviral Hops Compounds against Chikungunya Virus |
title_sort | identification of potential antiviral hops compounds against chikungunya virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966109/ https://www.ncbi.nlm.nih.gov/pubmed/36834745 http://dx.doi.org/10.3390/ijms24043333 |
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