Cargando…

Biodegradation of Selected Hydrocarbons by Fusarium Species Isolated from Contaminated Soil Samples in Riyadh, Saudi Arabia

Background: Microbial biodegradation of oil-hydrocarbons is one of the sustainable and cost-effective methods to remove petroleum spills from contaminated environments. The current study aimed to investigate the biodegradation abilities of three Fusarium isolates from oil reservoirs in Saudi Arabia....

Descripción completa

Detalles Bibliográficos
Autores principales: Al-Otibi, Fatimah, Al-Zahrani, Rasha M., Marraiki, Najat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966121/
https://www.ncbi.nlm.nih.gov/pubmed/36836330
http://dx.doi.org/10.3390/jof9020216
_version_ 1784896937123643392
author Al-Otibi, Fatimah
Al-Zahrani, Rasha M.
Marraiki, Najat
author_facet Al-Otibi, Fatimah
Al-Zahrani, Rasha M.
Marraiki, Najat
author_sort Al-Otibi, Fatimah
collection PubMed
description Background: Microbial biodegradation of oil-hydrocarbons is one of the sustainable and cost-effective methods to remove petroleum spills from contaminated environments. The current study aimed to investigate the biodegradation abilities of three Fusarium isolates from oil reservoirs in Saudi Arabia. The novelty of the current work is that the biodegradation ability of these isolates was never tested against some natural hydrocarbons of variable compositions, such as Crude oil, and those of known components such as kerosene and diesel oils. Methods: The isolates were treated with five selected hydrocarbons. The hydrocarbon tolerance test in solid and liquid media was performed. The scanning electron microscope (SEM) investigated the morphological changes of treated fungi. 2, 6-Dichlorophenol Indophenol (DCPIP), drop collapse, emulsification activity, and oil Spreading assays investigated the biodegradation ability. The amount of produced biosurfactants was measured, and their safety profile was estimated by the germination assay of tomato seeds. Results: The tolerance test showed enhanced fungal growth of all isolates, whereas the highest dose inhibition response (DIR) was 77% for Fusarium proliferatum treated with the used oil (p < 0.05). SEM showed morphological changes in all isolates. DCPIP results showed that used oil had the highest biodegradation by Fusarium verticillioides and Fusarium oxysporum. Mixed oil induced the highest effect in oil spreading, drop collapse, and emulsification assay caused by F. proliferatum. The highest recovery of biosurfactants was obtained by the solvent extraction method for F. verticillioides (4.6 g/L), F. proliferatum (4.22 g/L), and F. oxysporum (3.73 g/L). The biosurfactants produced by the three isolates stimulated tomato seeds’ germination more than in control experiments. Conclusion: The current study suggested the possible oil-biodegradation activities induced by three Fusarium isolates from Riyadh, Saudi Arabia. The produced biosurfactants are not toxic against tomato seed germination, emphasizing their environmental sustainability. Further studies are required to investigate the mechanism of biodegradation activities and the chemical composition of the biosurfactants produced by these species.
format Online
Article
Text
id pubmed-9966121
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99661212023-02-26 Biodegradation of Selected Hydrocarbons by Fusarium Species Isolated from Contaminated Soil Samples in Riyadh, Saudi Arabia Al-Otibi, Fatimah Al-Zahrani, Rasha M. Marraiki, Najat J Fungi (Basel) Article Background: Microbial biodegradation of oil-hydrocarbons is one of the sustainable and cost-effective methods to remove petroleum spills from contaminated environments. The current study aimed to investigate the biodegradation abilities of three Fusarium isolates from oil reservoirs in Saudi Arabia. The novelty of the current work is that the biodegradation ability of these isolates was never tested against some natural hydrocarbons of variable compositions, such as Crude oil, and those of known components such as kerosene and diesel oils. Methods: The isolates were treated with five selected hydrocarbons. The hydrocarbon tolerance test in solid and liquid media was performed. The scanning electron microscope (SEM) investigated the morphological changes of treated fungi. 2, 6-Dichlorophenol Indophenol (DCPIP), drop collapse, emulsification activity, and oil Spreading assays investigated the biodegradation ability. The amount of produced biosurfactants was measured, and their safety profile was estimated by the germination assay of tomato seeds. Results: The tolerance test showed enhanced fungal growth of all isolates, whereas the highest dose inhibition response (DIR) was 77% for Fusarium proliferatum treated with the used oil (p < 0.05). SEM showed morphological changes in all isolates. DCPIP results showed that used oil had the highest biodegradation by Fusarium verticillioides and Fusarium oxysporum. Mixed oil induced the highest effect in oil spreading, drop collapse, and emulsification assay caused by F. proliferatum. The highest recovery of biosurfactants was obtained by the solvent extraction method for F. verticillioides (4.6 g/L), F. proliferatum (4.22 g/L), and F. oxysporum (3.73 g/L). The biosurfactants produced by the three isolates stimulated tomato seeds’ germination more than in control experiments. Conclusion: The current study suggested the possible oil-biodegradation activities induced by three Fusarium isolates from Riyadh, Saudi Arabia. The produced biosurfactants are not toxic against tomato seed germination, emphasizing their environmental sustainability. Further studies are required to investigate the mechanism of biodegradation activities and the chemical composition of the biosurfactants produced by these species. MDPI 2023-02-06 /pmc/articles/PMC9966121/ /pubmed/36836330 http://dx.doi.org/10.3390/jof9020216 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Otibi, Fatimah
Al-Zahrani, Rasha M.
Marraiki, Najat
Biodegradation of Selected Hydrocarbons by Fusarium Species Isolated from Contaminated Soil Samples in Riyadh, Saudi Arabia
title Biodegradation of Selected Hydrocarbons by Fusarium Species Isolated from Contaminated Soil Samples in Riyadh, Saudi Arabia
title_full Biodegradation of Selected Hydrocarbons by Fusarium Species Isolated from Contaminated Soil Samples in Riyadh, Saudi Arabia
title_fullStr Biodegradation of Selected Hydrocarbons by Fusarium Species Isolated from Contaminated Soil Samples in Riyadh, Saudi Arabia
title_full_unstemmed Biodegradation of Selected Hydrocarbons by Fusarium Species Isolated from Contaminated Soil Samples in Riyadh, Saudi Arabia
title_short Biodegradation of Selected Hydrocarbons by Fusarium Species Isolated from Contaminated Soil Samples in Riyadh, Saudi Arabia
title_sort biodegradation of selected hydrocarbons by fusarium species isolated from contaminated soil samples in riyadh, saudi arabia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966121/
https://www.ncbi.nlm.nih.gov/pubmed/36836330
http://dx.doi.org/10.3390/jof9020216
work_keys_str_mv AT alotibifatimah biodegradationofselectedhydrocarbonsbyfusariumspeciesisolatedfromcontaminatedsoilsamplesinriyadhsaudiarabia
AT alzahranirasham biodegradationofselectedhydrocarbonsbyfusariumspeciesisolatedfromcontaminatedsoilsamplesinriyadhsaudiarabia
AT marraikinajat biodegradationofselectedhydrocarbonsbyfusariumspeciesisolatedfromcontaminatedsoilsamplesinriyadhsaudiarabia