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NMR-Based Metabolite Profiling and the Application of STOCSY toward the Quality and Authentication Assessment of European EVOOs

Extra virgin olive oil (EVOO) possesses a high-value rank in the food industry, thus making it a common target for adulteration. Hence, several methods have been essentially made available over the years. However, the issue of authentication remains unresolved with national and food safety organizat...

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Autores principales: Beteinakis, Stavros, Papachristodoulou, Anastasia, Kolb, Peter, Rösch, Paul, Schwarzinger, Stephan, Mikros, Emmanuel, Halabalaki, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966212/
https://www.ncbi.nlm.nih.gov/pubmed/36838725
http://dx.doi.org/10.3390/molecules28041738
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author Beteinakis, Stavros
Papachristodoulou, Anastasia
Kolb, Peter
Rösch, Paul
Schwarzinger, Stephan
Mikros, Emmanuel
Halabalaki, Maria
author_facet Beteinakis, Stavros
Papachristodoulou, Anastasia
Kolb, Peter
Rösch, Paul
Schwarzinger, Stephan
Mikros, Emmanuel
Halabalaki, Maria
author_sort Beteinakis, Stavros
collection PubMed
description Extra virgin olive oil (EVOO) possesses a high-value rank in the food industry, thus making it a common target for adulteration. Hence, several methods have been essentially made available over the years. However, the issue of authentication remains unresolved with national and food safety organizations globally struggling to regulate and control its market. Over the course of this study, the aim was to determine the origin of EVOOs suggesting a high-throughput, state-of-the-art method that could be easily adopted. A rapid, NMR-based untargeted metabolite profiling method was applied and complemented by multivariate analysis (MVA) and statistical total correlation spectroscopy (STOCSY). STOCSY is a valuable statistical tool contributing to the biomarker identification process and was employed for the first time in EVOO analysis. Market samples from three Mediterranean countries of Spain, Italy, and Greece, blended samples from these countries, as well as monocultivar samples from Greece were analyzed. The NMR spectra were collected, with the help of chemometrics acting as “fingerprints” leading to the discovery of certain chemical classes and single biomarkers that were related to the classification of the samples into groups based on their origin.
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spelling pubmed-99662122023-02-26 NMR-Based Metabolite Profiling and the Application of STOCSY toward the Quality and Authentication Assessment of European EVOOs Beteinakis, Stavros Papachristodoulou, Anastasia Kolb, Peter Rösch, Paul Schwarzinger, Stephan Mikros, Emmanuel Halabalaki, Maria Molecules Article Extra virgin olive oil (EVOO) possesses a high-value rank in the food industry, thus making it a common target for adulteration. Hence, several methods have been essentially made available over the years. However, the issue of authentication remains unresolved with national and food safety organizations globally struggling to regulate and control its market. Over the course of this study, the aim was to determine the origin of EVOOs suggesting a high-throughput, state-of-the-art method that could be easily adopted. A rapid, NMR-based untargeted metabolite profiling method was applied and complemented by multivariate analysis (MVA) and statistical total correlation spectroscopy (STOCSY). STOCSY is a valuable statistical tool contributing to the biomarker identification process and was employed for the first time in EVOO analysis. Market samples from three Mediterranean countries of Spain, Italy, and Greece, blended samples from these countries, as well as monocultivar samples from Greece were analyzed. The NMR spectra were collected, with the help of chemometrics acting as “fingerprints” leading to the discovery of certain chemical classes and single biomarkers that were related to the classification of the samples into groups based on their origin. MDPI 2023-02-11 /pmc/articles/PMC9966212/ /pubmed/36838725 http://dx.doi.org/10.3390/molecules28041738 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Beteinakis, Stavros
Papachristodoulou, Anastasia
Kolb, Peter
Rösch, Paul
Schwarzinger, Stephan
Mikros, Emmanuel
Halabalaki, Maria
NMR-Based Metabolite Profiling and the Application of STOCSY toward the Quality and Authentication Assessment of European EVOOs
title NMR-Based Metabolite Profiling and the Application of STOCSY toward the Quality and Authentication Assessment of European EVOOs
title_full NMR-Based Metabolite Profiling and the Application of STOCSY toward the Quality and Authentication Assessment of European EVOOs
title_fullStr NMR-Based Metabolite Profiling and the Application of STOCSY toward the Quality and Authentication Assessment of European EVOOs
title_full_unstemmed NMR-Based Metabolite Profiling and the Application of STOCSY toward the Quality and Authentication Assessment of European EVOOs
title_short NMR-Based Metabolite Profiling and the Application of STOCSY toward the Quality and Authentication Assessment of European EVOOs
title_sort nmr-based metabolite profiling and the application of stocsy toward the quality and authentication assessment of european evoos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966212/
https://www.ncbi.nlm.nih.gov/pubmed/36838725
http://dx.doi.org/10.3390/molecules28041738
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