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Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids
This paper considers the evaluation of uncertainty of quantitative gel electrophoresis. To date, such uncertainty estimation presented in the literature are based on the multiple measurements performed for assessing the intra- and interlaboratory reproducibility using standard samples. This paper sh...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966319/ https://www.ncbi.nlm.nih.gov/pubmed/36850595 http://dx.doi.org/10.3390/s23041999 |
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author | Semenov, Konstantin Taraskin, Aleksandr Yurchenko, Alexandra Baranovskaya, Irina Purvinsh, Lada Gyulikhandanova, Natalia Vasin, Andrey |
author_facet | Semenov, Konstantin Taraskin, Aleksandr Yurchenko, Alexandra Baranovskaya, Irina Purvinsh, Lada Gyulikhandanova, Natalia Vasin, Andrey |
author_sort | Semenov, Konstantin |
collection | PubMed |
description | This paper considers the evaluation of uncertainty of quantitative gel electrophoresis. To date, such uncertainty estimation presented in the literature are based on the multiple measurements performed for assessing the intra- and interlaboratory reproducibility using standard samples. This paper shows how to estimate the uncertainty in cases where we cannot study scattering components of the results. The first point is dedicated to a case where we have standard samples (the direct expressions are shown). The second point considers the situation when standard samples are absent (the algorithm for estimating the lower bound for uncertainty is discussed). The role of the data processing algorithm is demonstrated. |
format | Online Article Text |
id | pubmed-9966319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99663192023-02-26 Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids Semenov, Konstantin Taraskin, Aleksandr Yurchenko, Alexandra Baranovskaya, Irina Purvinsh, Lada Gyulikhandanova, Natalia Vasin, Andrey Sensors (Basel) Communication This paper considers the evaluation of uncertainty of quantitative gel electrophoresis. To date, such uncertainty estimation presented in the literature are based on the multiple measurements performed for assessing the intra- and interlaboratory reproducibility using standard samples. This paper shows how to estimate the uncertainty in cases where we cannot study scattering components of the results. The first point is dedicated to a case where we have standard samples (the direct expressions are shown). The second point considers the situation when standard samples are absent (the algorithm for estimating the lower bound for uncertainty is discussed). The role of the data processing algorithm is demonstrated. MDPI 2023-02-10 /pmc/articles/PMC9966319/ /pubmed/36850595 http://dx.doi.org/10.3390/s23041999 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Semenov, Konstantin Taraskin, Aleksandr Yurchenko, Alexandra Baranovskaya, Irina Purvinsh, Lada Gyulikhandanova, Natalia Vasin, Andrey Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids |
title | Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids |
title_full | Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids |
title_fullStr | Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids |
title_full_unstemmed | Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids |
title_short | Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids |
title_sort | uncertainty estimation for quantitative agarose gel electrophoresis of nucleic acids |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966319/ https://www.ncbi.nlm.nih.gov/pubmed/36850595 http://dx.doi.org/10.3390/s23041999 |
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