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Tumor Microenvironment and Genes Affecting the Prognosis of Temozolomide-Treated Glioblastoma

Glioblastoma (GBM) is the most frequent primary brain tumor in adults and has a poor prognosis due to its resistance to Temozolomide (TMZ). However, there is limited research regarding the tumor microenvironment and genes related to the prognosis of TMZ-treated GBM patients. This study aimed to iden...

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Autores principales: Jang, Yena, Cheong, Wooyong, Park, Gyurin, Kim, Yeongmin, Ha, Junbeom, Ahn, Sangzin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966340/
https://www.ncbi.nlm.nih.gov/pubmed/36836422
http://dx.doi.org/10.3390/jpm13020188
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author Jang, Yena
Cheong, Wooyong
Park, Gyurin
Kim, Yeongmin
Ha, Junbeom
Ahn, Sangzin
author_facet Jang, Yena
Cheong, Wooyong
Park, Gyurin
Kim, Yeongmin
Ha, Junbeom
Ahn, Sangzin
author_sort Jang, Yena
collection PubMed
description Glioblastoma (GBM) is the most frequent primary brain tumor in adults and has a poor prognosis due to its resistance to Temozolomide (TMZ). However, there is limited research regarding the tumor microenvironment and genes related to the prognosis of TMZ-treated GBM patients. This study aimed to identify putative transcriptomic biomarkers with predictive value in patients with GBM who were treated with TMZ. Publicly available datasets from The Cancer Genome Atlas and Gene Expression Omnibus were analyzed using CIBERSORTx and Weighted Gene Co-expression Network Analysis (WGCNA) to obtain types of highly expressed cell types and gene clusters. Differentially Expressed Genes analysis was performed and was intersected with the WGCNA results to obtain a candidate gene list. Cox proportional-hazard survival analysis was performed to acquire genes related to the prognosis of TMZ-treated GBM patients. Inflammatory microglial cells, dendritic cells, myeloid cells, and glioma stem cells were highly expressed in GBM tissue, and ACP7, EPPK1, PCDHA8, RHOD, DRC1, ZIC3, and PRLR were significantly associated with survival. While the listed genes have been previously reported to be related to glioblastoma or other types of cancer, ACP7 was identified as a novel gene related to the prognosis of GBM. These findings may have potential implications for developing a diagnostic tool to predict GBM resistance and optimize treatment decisions.
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spelling pubmed-99663402023-02-26 Tumor Microenvironment and Genes Affecting the Prognosis of Temozolomide-Treated Glioblastoma Jang, Yena Cheong, Wooyong Park, Gyurin Kim, Yeongmin Ha, Junbeom Ahn, Sangzin J Pers Med Article Glioblastoma (GBM) is the most frequent primary brain tumor in adults and has a poor prognosis due to its resistance to Temozolomide (TMZ). However, there is limited research regarding the tumor microenvironment and genes related to the prognosis of TMZ-treated GBM patients. This study aimed to identify putative transcriptomic biomarkers with predictive value in patients with GBM who were treated with TMZ. Publicly available datasets from The Cancer Genome Atlas and Gene Expression Omnibus were analyzed using CIBERSORTx and Weighted Gene Co-expression Network Analysis (WGCNA) to obtain types of highly expressed cell types and gene clusters. Differentially Expressed Genes analysis was performed and was intersected with the WGCNA results to obtain a candidate gene list. Cox proportional-hazard survival analysis was performed to acquire genes related to the prognosis of TMZ-treated GBM patients. Inflammatory microglial cells, dendritic cells, myeloid cells, and glioma stem cells were highly expressed in GBM tissue, and ACP7, EPPK1, PCDHA8, RHOD, DRC1, ZIC3, and PRLR were significantly associated with survival. While the listed genes have been previously reported to be related to glioblastoma or other types of cancer, ACP7 was identified as a novel gene related to the prognosis of GBM. These findings may have potential implications for developing a diagnostic tool to predict GBM resistance and optimize treatment decisions. MDPI 2023-01-20 /pmc/articles/PMC9966340/ /pubmed/36836422 http://dx.doi.org/10.3390/jpm13020188 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jang, Yena
Cheong, Wooyong
Park, Gyurin
Kim, Yeongmin
Ha, Junbeom
Ahn, Sangzin
Tumor Microenvironment and Genes Affecting the Prognosis of Temozolomide-Treated Glioblastoma
title Tumor Microenvironment and Genes Affecting the Prognosis of Temozolomide-Treated Glioblastoma
title_full Tumor Microenvironment and Genes Affecting the Prognosis of Temozolomide-Treated Glioblastoma
title_fullStr Tumor Microenvironment and Genes Affecting the Prognosis of Temozolomide-Treated Glioblastoma
title_full_unstemmed Tumor Microenvironment and Genes Affecting the Prognosis of Temozolomide-Treated Glioblastoma
title_short Tumor Microenvironment and Genes Affecting the Prognosis of Temozolomide-Treated Glioblastoma
title_sort tumor microenvironment and genes affecting the prognosis of temozolomide-treated glioblastoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966340/
https://www.ncbi.nlm.nih.gov/pubmed/36836422
http://dx.doi.org/10.3390/jpm13020188
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