Cargando…
Mechanisms of Arachidonic Acid In Vitro Tumoricidal Impact
To promote the potential of arachidonic acid (ARA) for cancer prevention and management, experiments were implemented to disclose the mechanisms of its tumoricidal action. Hepatocellular, lung, and breast carcinoma and normal hepatocytes cell lines were exposed to 0 or 50 μM ARA for 30 min and then...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966399/ https://www.ncbi.nlm.nih.gov/pubmed/36838715 http://dx.doi.org/10.3390/molecules28041727 |
_version_ | 1784897007210463232 |
---|---|
author | Tallima, Hatem El Ridi, Rashika |
author_facet | Tallima, Hatem El Ridi, Rashika |
author_sort | Tallima, Hatem |
collection | PubMed |
description | To promote the potential of arachidonic acid (ARA) for cancer prevention and management, experiments were implemented to disclose the mechanisms of its tumoricidal action. Hepatocellular, lung, and breast carcinoma and normal hepatocytes cell lines were exposed to 0 or 50 μM ARA for 30 min and then assessed for proliferative capacity, surface membrane-associated sphingomyelin (SM) content, neutral sphingomyelinase (nSMase) activity, beta 2 microglobulin (β2 m) expression, and ceramide (Cer) levels. Reactive oxygen species (ROS) content and caspase 3/7 activity were evaluated. Exposure to ARA for 30 min led to impairment of the tumor cells’ proliferative capacity and revealed that the different cell lines display remarkably similar surface membrane SM content but diverse responses to ARA treatment. Arachidonic acid tumoricidal impact was shown to be associated with nSMase activation, exposure of cell surface membrane β2 m to antibody binding, and hydrolysis of SM to Cer, which accumulated on the cell surface and in the cytosol. The ARA and Cer-mediated inhibition of tumor cell viability appeared to be independent of ROS generation or caspase 3/7 activation. The data were compared and contrasted to findings reported in the literature on ARA tumoricidal mechanisms. |
format | Online Article Text |
id | pubmed-9966399 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99663992023-02-26 Mechanisms of Arachidonic Acid In Vitro Tumoricidal Impact Tallima, Hatem El Ridi, Rashika Molecules Article To promote the potential of arachidonic acid (ARA) for cancer prevention and management, experiments were implemented to disclose the mechanisms of its tumoricidal action. Hepatocellular, lung, and breast carcinoma and normal hepatocytes cell lines were exposed to 0 or 50 μM ARA for 30 min and then assessed for proliferative capacity, surface membrane-associated sphingomyelin (SM) content, neutral sphingomyelinase (nSMase) activity, beta 2 microglobulin (β2 m) expression, and ceramide (Cer) levels. Reactive oxygen species (ROS) content and caspase 3/7 activity were evaluated. Exposure to ARA for 30 min led to impairment of the tumor cells’ proliferative capacity and revealed that the different cell lines display remarkably similar surface membrane SM content but diverse responses to ARA treatment. Arachidonic acid tumoricidal impact was shown to be associated with nSMase activation, exposure of cell surface membrane β2 m to antibody binding, and hydrolysis of SM to Cer, which accumulated on the cell surface and in the cytosol. The ARA and Cer-mediated inhibition of tumor cell viability appeared to be independent of ROS generation or caspase 3/7 activation. The data were compared and contrasted to findings reported in the literature on ARA tumoricidal mechanisms. MDPI 2023-02-11 /pmc/articles/PMC9966399/ /pubmed/36838715 http://dx.doi.org/10.3390/molecules28041727 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tallima, Hatem El Ridi, Rashika Mechanisms of Arachidonic Acid In Vitro Tumoricidal Impact |
title | Mechanisms of Arachidonic Acid In Vitro Tumoricidal Impact |
title_full | Mechanisms of Arachidonic Acid In Vitro Tumoricidal Impact |
title_fullStr | Mechanisms of Arachidonic Acid In Vitro Tumoricidal Impact |
title_full_unstemmed | Mechanisms of Arachidonic Acid In Vitro Tumoricidal Impact |
title_short | Mechanisms of Arachidonic Acid In Vitro Tumoricidal Impact |
title_sort | mechanisms of arachidonic acid in vitro tumoricidal impact |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966399/ https://www.ncbi.nlm.nih.gov/pubmed/36838715 http://dx.doi.org/10.3390/molecules28041727 |
work_keys_str_mv | AT tallimahatem mechanismsofarachidonicacidinvitrotumoricidalimpact AT elridirashika mechanismsofarachidonicacidinvitrotumoricidalimpact |