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Presence of Anaplasma phagocytophilum Ecotype I in UK Ruminants and Associated Zoonotic Risk
Anaplasma phagocytophilum is the causative agent of tick-borne fever in sheep, pasture fever in cattle, and granulocytic anaplasmosis in humans. The increasing prevalence and transboundary spread of A. phagocytophilum in livestock, ticks, and wildlife in the UK poses a potential zoonotic risk that h...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966478/ https://www.ncbi.nlm.nih.gov/pubmed/36839488 http://dx.doi.org/10.3390/pathogens12020216 |
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author | Bianchessi, Laura Rocchi, Mara Silvia Maley, Madeleine Allen, Kayleigh Ballingall, Keith Turin, Lauretta |
author_facet | Bianchessi, Laura Rocchi, Mara Silvia Maley, Madeleine Allen, Kayleigh Ballingall, Keith Turin, Lauretta |
author_sort | Bianchessi, Laura |
collection | PubMed |
description | Anaplasma phagocytophilum is the causative agent of tick-borne fever in sheep, pasture fever in cattle, and granulocytic anaplasmosis in humans. The increasing prevalence and transboundary spread of A. phagocytophilum in livestock, ticks, and wildlife in the UK poses a potential zoonotic risk that has yet to be estimated. Several ecotypes of A. phagocytophilum show variable zoonotic potential. To evaluate the possible risk associated with the transmission of A. phagocytophilum from ruminants to humans, the ecotype was determined by sequencing the groEL gene from 71 positive blood and tissue samples from UK ruminants. Thirty-four groEL sequences were obtained, fourteen of which were identified in multiple samples. Of the 13 nucleotide polymorphisms identified through pairwise comparison, all corresponded to synonymous substitutions. The subsequent phylogenetic estimation of the relationship with other European/world isolates indicated that all the groEL sequences clustered with other ecotype I sequences. The presence of ecotype I closely reflects that observed in ruminants in continental Europe and suggests a lower risk of zoonotic transmission from this reservoir. |
format | Online Article Text |
id | pubmed-9966478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99664782023-02-26 Presence of Anaplasma phagocytophilum Ecotype I in UK Ruminants and Associated Zoonotic Risk Bianchessi, Laura Rocchi, Mara Silvia Maley, Madeleine Allen, Kayleigh Ballingall, Keith Turin, Lauretta Pathogens Article Anaplasma phagocytophilum is the causative agent of tick-borne fever in sheep, pasture fever in cattle, and granulocytic anaplasmosis in humans. The increasing prevalence and transboundary spread of A. phagocytophilum in livestock, ticks, and wildlife in the UK poses a potential zoonotic risk that has yet to be estimated. Several ecotypes of A. phagocytophilum show variable zoonotic potential. To evaluate the possible risk associated with the transmission of A. phagocytophilum from ruminants to humans, the ecotype was determined by sequencing the groEL gene from 71 positive blood and tissue samples from UK ruminants. Thirty-four groEL sequences were obtained, fourteen of which were identified in multiple samples. Of the 13 nucleotide polymorphisms identified through pairwise comparison, all corresponded to synonymous substitutions. The subsequent phylogenetic estimation of the relationship with other European/world isolates indicated that all the groEL sequences clustered with other ecotype I sequences. The presence of ecotype I closely reflects that observed in ruminants in continental Europe and suggests a lower risk of zoonotic transmission from this reservoir. MDPI 2023-01-30 /pmc/articles/PMC9966478/ /pubmed/36839488 http://dx.doi.org/10.3390/pathogens12020216 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bianchessi, Laura Rocchi, Mara Silvia Maley, Madeleine Allen, Kayleigh Ballingall, Keith Turin, Lauretta Presence of Anaplasma phagocytophilum Ecotype I in UK Ruminants and Associated Zoonotic Risk |
title | Presence of Anaplasma phagocytophilum Ecotype I in UK Ruminants and Associated Zoonotic Risk |
title_full | Presence of Anaplasma phagocytophilum Ecotype I in UK Ruminants and Associated Zoonotic Risk |
title_fullStr | Presence of Anaplasma phagocytophilum Ecotype I in UK Ruminants and Associated Zoonotic Risk |
title_full_unstemmed | Presence of Anaplasma phagocytophilum Ecotype I in UK Ruminants and Associated Zoonotic Risk |
title_short | Presence of Anaplasma phagocytophilum Ecotype I in UK Ruminants and Associated Zoonotic Risk |
title_sort | presence of anaplasma phagocytophilum ecotype i in uk ruminants and associated zoonotic risk |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966478/ https://www.ncbi.nlm.nih.gov/pubmed/36839488 http://dx.doi.org/10.3390/pathogens12020216 |
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