Cargando…

Oral Wound Healing Potential of Polygoni Cuspidati Rhizoma et Radix Decoction—In Vitro Study

Polygoni Cuspidati Rhizoma et Radix (syn. rhizomes of Reynoutria japonica Houtt.) is a pharmacopoeial raw material in Europe and China. In traditional medicine, one of the applications for Reynoutria japonica rhizomes is wound healing. In a recent in vitro study, we demonstrated that ethanol and ace...

Descripción completa

Detalles Bibliográficos
Autores principales: Hadzik, Jakub, Choromańska, Anna, Karolewicz, Bożena, Matkowski, Adam, Dominiak, Marzena, Złocińska, Adrianna, Nawrot-Hadzik, Izabela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966654/
https://www.ncbi.nlm.nih.gov/pubmed/37259412
http://dx.doi.org/10.3390/ph16020267
_version_ 1784897070713274368
author Hadzik, Jakub
Choromańska, Anna
Karolewicz, Bożena
Matkowski, Adam
Dominiak, Marzena
Złocińska, Adrianna
Nawrot-Hadzik, Izabela
author_facet Hadzik, Jakub
Choromańska, Anna
Karolewicz, Bożena
Matkowski, Adam
Dominiak, Marzena
Złocińska, Adrianna
Nawrot-Hadzik, Izabela
author_sort Hadzik, Jakub
collection PubMed
description Polygoni Cuspidati Rhizoma et Radix (syn. rhizomes of Reynoutria japonica Houtt.) is a pharmacopoeial raw material in Europe and China. In traditional medicine, one of the applications for Reynoutria japonica rhizomes is wound healing. In a recent in vitro study, we demonstrated that ethanol and acetone extracts from this herbal drug have the potential to heal oral gum wounds. However, considering that a majority of herbal medicines have been traditionally administered as water decoctions, in the present study, a decoction of Reynoutria japonica rhizomes was prepared and detailed tests to determine its in vitro gingival wound healing activity were conducted. We used the primary human gingival fibroblasts (HGF) incubated with a decoction to determine cell viability (MTT assay), cell proliferation (the confocal laser scanning microscope—CLSM), and cell migration (wound healing assay). Moreover, the collagen type III expression was examined using immunocytochemical staining. The studied decoction was qualitatively and quantitatively characterized using the validated HPLC/DAD/ESI-HR-QTOF-MS method. The Folin–Ciocalteu assay was used to determine the total phenols and tannins content. Additionally, HPLC-RI analysis of decoction and the previously obtained ethanol and acetone extracts was used to determine the composition of saccharides. Low concentration (from 50 to 1000 µg/mL) of decoction after 24 h incubation caused a significant increase in HGF cell viability. No cytotoxic effect was observed at any tested concentration (up to 2000 µg/mL). The lowest active concentration of decoction (50 µg/mL) was selected for further experiments. It significantly stimulated human gingival fibroblasts to proliferate, migrate, and increase the synthesis of collagen III. Phytochemical analysis showed significantly fewer polyphenols in the decoction than in the ethanol and acetone extracts tested earlier. In contrast, high levels of polysaccharides were observed. In our opinion, they may have a significant effect on the oral wound healing parameters analyzed in vitro. The results obtained encourage the use of this raw material in its traditional, safe form—decoction.
format Online
Article
Text
id pubmed-9966654
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99666542023-02-26 Oral Wound Healing Potential of Polygoni Cuspidati Rhizoma et Radix Decoction—In Vitro Study Hadzik, Jakub Choromańska, Anna Karolewicz, Bożena Matkowski, Adam Dominiak, Marzena Złocińska, Adrianna Nawrot-Hadzik, Izabela Pharmaceuticals (Basel) Article Polygoni Cuspidati Rhizoma et Radix (syn. rhizomes of Reynoutria japonica Houtt.) is a pharmacopoeial raw material in Europe and China. In traditional medicine, one of the applications for Reynoutria japonica rhizomes is wound healing. In a recent in vitro study, we demonstrated that ethanol and acetone extracts from this herbal drug have the potential to heal oral gum wounds. However, considering that a majority of herbal medicines have been traditionally administered as water decoctions, in the present study, a decoction of Reynoutria japonica rhizomes was prepared and detailed tests to determine its in vitro gingival wound healing activity were conducted. We used the primary human gingival fibroblasts (HGF) incubated with a decoction to determine cell viability (MTT assay), cell proliferation (the confocal laser scanning microscope—CLSM), and cell migration (wound healing assay). Moreover, the collagen type III expression was examined using immunocytochemical staining. The studied decoction was qualitatively and quantitatively characterized using the validated HPLC/DAD/ESI-HR-QTOF-MS method. The Folin–Ciocalteu assay was used to determine the total phenols and tannins content. Additionally, HPLC-RI analysis of decoction and the previously obtained ethanol and acetone extracts was used to determine the composition of saccharides. Low concentration (from 50 to 1000 µg/mL) of decoction after 24 h incubation caused a significant increase in HGF cell viability. No cytotoxic effect was observed at any tested concentration (up to 2000 µg/mL). The lowest active concentration of decoction (50 µg/mL) was selected for further experiments. It significantly stimulated human gingival fibroblasts to proliferate, migrate, and increase the synthesis of collagen III. Phytochemical analysis showed significantly fewer polyphenols in the decoction than in the ethanol and acetone extracts tested earlier. In contrast, high levels of polysaccharides were observed. In our opinion, they may have a significant effect on the oral wound healing parameters analyzed in vitro. The results obtained encourage the use of this raw material in its traditional, safe form—decoction. MDPI 2023-02-10 /pmc/articles/PMC9966654/ /pubmed/37259412 http://dx.doi.org/10.3390/ph16020267 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hadzik, Jakub
Choromańska, Anna
Karolewicz, Bożena
Matkowski, Adam
Dominiak, Marzena
Złocińska, Adrianna
Nawrot-Hadzik, Izabela
Oral Wound Healing Potential of Polygoni Cuspidati Rhizoma et Radix Decoction—In Vitro Study
title Oral Wound Healing Potential of Polygoni Cuspidati Rhizoma et Radix Decoction—In Vitro Study
title_full Oral Wound Healing Potential of Polygoni Cuspidati Rhizoma et Radix Decoction—In Vitro Study
title_fullStr Oral Wound Healing Potential of Polygoni Cuspidati Rhizoma et Radix Decoction—In Vitro Study
title_full_unstemmed Oral Wound Healing Potential of Polygoni Cuspidati Rhizoma et Radix Decoction—In Vitro Study
title_short Oral Wound Healing Potential of Polygoni Cuspidati Rhizoma et Radix Decoction—In Vitro Study
title_sort oral wound healing potential of polygoni cuspidati rhizoma et radix decoction—in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966654/
https://www.ncbi.nlm.nih.gov/pubmed/37259412
http://dx.doi.org/10.3390/ph16020267
work_keys_str_mv AT hadzikjakub oralwoundhealingpotentialofpolygonicuspidatirhizomaetradixdecoctioninvitrostudy
AT choromanskaanna oralwoundhealingpotentialofpolygonicuspidatirhizomaetradixdecoctioninvitrostudy
AT karolewiczbozena oralwoundhealingpotentialofpolygonicuspidatirhizomaetradixdecoctioninvitrostudy
AT matkowskiadam oralwoundhealingpotentialofpolygonicuspidatirhizomaetradixdecoctioninvitrostudy
AT dominiakmarzena oralwoundhealingpotentialofpolygonicuspidatirhizomaetradixdecoctioninvitrostudy
AT złocinskaadrianna oralwoundhealingpotentialofpolygonicuspidatirhizomaetradixdecoctioninvitrostudy
AT nawrothadzikizabela oralwoundhealingpotentialofpolygonicuspidatirhizomaetradixdecoctioninvitrostudy