Cargando…
Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA
Gamma-aminobutyric acid (GABA) is a functional metabolite in various organisms. Herein, a sensitivity intensified ninhydrin-based chromogenic system (SINICS), achieved by ethanol and ethyl acetate, is described for the reliable relative quantitation of GABA. A 2.9 mL SINICS kit comprises 1% ninhydri...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966720/ https://www.ncbi.nlm.nih.gov/pubmed/36837902 http://dx.doi.org/10.3390/metabo13020283 |
_version_ | 1784897087167528960 |
---|---|
author | Li, Haixing Wang, Lingqin Nie, Lijuan Liu, Xiaohua Fu, Jinheng |
author_facet | Li, Haixing Wang, Lingqin Nie, Lijuan Liu, Xiaohua Fu, Jinheng |
author_sort | Li, Haixing |
collection | PubMed |
description | Gamma-aminobutyric acid (GABA) is a functional metabolite in various organisms. Herein, a sensitivity intensified ninhydrin-based chromogenic system (SINICS), achieved by ethanol and ethyl acetate, is described for the reliable relative quantitation of GABA. A 2.9 mL SINICS kit comprises 1% ninhydrin, 40% ethanol, 25% ethyl acetate, and 35 μL 0.2 M sodium acetate buffer (pH 5.0). In practice, following the addition of a 0.1 mL sample to the kit, the chromogenic reaction is completed by heating at 70 °C for 30 min. The kit increased the color development sensitivity of L-glutamic acid and GABA, with the detection limits being reduced from 20 mM and 200 mM to 5 mM and 20 mM, respectively. The chromophore was stable for at least 2 h at room temperature, which was sufficient for a routine colorimetric analysis. The absorbance at 570 nm with the deduction of background directly represents the content of amino acid. For a proof-of-concept, the SINICS was adopted to optimize the GABA fermentation process of Levilactobacillus brevis CD0817. The results demonstrated that SINICS is an attractive alternative to the available ninhydrin-based colorimetric methods. |
format | Online Article Text |
id | pubmed-9966720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99667202023-02-26 Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA Li, Haixing Wang, Lingqin Nie, Lijuan Liu, Xiaohua Fu, Jinheng Metabolites Article Gamma-aminobutyric acid (GABA) is a functional metabolite in various organisms. Herein, a sensitivity intensified ninhydrin-based chromogenic system (SINICS), achieved by ethanol and ethyl acetate, is described for the reliable relative quantitation of GABA. A 2.9 mL SINICS kit comprises 1% ninhydrin, 40% ethanol, 25% ethyl acetate, and 35 μL 0.2 M sodium acetate buffer (pH 5.0). In practice, following the addition of a 0.1 mL sample to the kit, the chromogenic reaction is completed by heating at 70 °C for 30 min. The kit increased the color development sensitivity of L-glutamic acid and GABA, with the detection limits being reduced from 20 mM and 200 mM to 5 mM and 20 mM, respectively. The chromophore was stable for at least 2 h at room temperature, which was sufficient for a routine colorimetric analysis. The absorbance at 570 nm with the deduction of background directly represents the content of amino acid. For a proof-of-concept, the SINICS was adopted to optimize the GABA fermentation process of Levilactobacillus brevis CD0817. The results demonstrated that SINICS is an attractive alternative to the available ninhydrin-based colorimetric methods. MDPI 2023-02-16 /pmc/articles/PMC9966720/ /pubmed/36837902 http://dx.doi.org/10.3390/metabo13020283 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Haixing Wang, Lingqin Nie, Lijuan Liu, Xiaohua Fu, Jinheng Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA |
title | Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA |
title_full | Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA |
title_fullStr | Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA |
title_full_unstemmed | Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA |
title_short | Sensitivity Intensified Ninhydrin-Based Chromogenic System by Ethanol-Ethyl Acetate: Application to Relative Quantitation of GABA |
title_sort | sensitivity intensified ninhydrin-based chromogenic system by ethanol-ethyl acetate: application to relative quantitation of gaba |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966720/ https://www.ncbi.nlm.nih.gov/pubmed/36837902 http://dx.doi.org/10.3390/metabo13020283 |
work_keys_str_mv | AT lihaixing sensitivityintensifiedninhydrinbasedchromogenicsystembyethanolethylacetateapplicationtorelativequantitationofgaba AT wanglingqin sensitivityintensifiedninhydrinbasedchromogenicsystembyethanolethylacetateapplicationtorelativequantitationofgaba AT nielijuan sensitivityintensifiedninhydrinbasedchromogenicsystembyethanolethylacetateapplicationtorelativequantitationofgaba AT liuxiaohua sensitivityintensifiedninhydrinbasedchromogenicsystembyethanolethylacetateapplicationtorelativequantitationofgaba AT fujinheng sensitivityintensifiedninhydrinbasedchromogenicsystembyethanolethylacetateapplicationtorelativequantitationofgaba |