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Molecular Characterization and Phylogenetic Analysis of Outer membrane protein P2 (OmpP2) of Glaesserella (Haemophilus) parasuis Isolates in Central State of Peninsular Malaysia

Glaesserella (Haemophilus) parasuis, the etiological agent of Glässer’s disease, is an economically significant pathogen commonly associated with serofibrinous polyserositis, arthritis, fibrinous bronchopneumonia and/or meningitis. This study is the first attempt to molecularly characterize and prov...

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Autores principales: Lee, Chee Yien, Ong, Hui Xin, Tan, Chew Yee, Low, Suet Ee, Phang, Lai Yee, Lai, Jyhmirn, Ooi, Peck Toung, Fong, Michelle Wai Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966854/
https://www.ncbi.nlm.nih.gov/pubmed/36839580
http://dx.doi.org/10.3390/pathogens12020308
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author Lee, Chee Yien
Ong, Hui Xin
Tan, Chew Yee
Low, Suet Ee
Phang, Lai Yee
Lai, Jyhmirn
Ooi, Peck Toung
Fong, Michelle Wai Cheng
author_facet Lee, Chee Yien
Ong, Hui Xin
Tan, Chew Yee
Low, Suet Ee
Phang, Lai Yee
Lai, Jyhmirn
Ooi, Peck Toung
Fong, Michelle Wai Cheng
author_sort Lee, Chee Yien
collection PubMed
description Glaesserella (Haemophilus) parasuis, the etiological agent of Glässer’s disease, is an economically significant pathogen commonly associated with serofibrinous polyserositis, arthritis, fibrinous bronchopneumonia and/or meningitis. This study is the first attempt to molecularly characterize and provide a detailed overview of the genetic variants of G. parasuis present in Malaysia, in reference to its serotype, virulence-associated trimeric autotransporters (vtaA) gene and outer membrane protein P2 (OmpP2) gene. The G. parasuis isolates (n = 11) from clinically sick field samples collected from two major pig producing states (Selangor and Perak) were selected for analysis. Upon multiplex PCR, the majority of the isolates (eight out of 11) were identified to be serotype 5 or 12, and interestingly, serotypes 3, 8 and 15 were also detected, which had never been reported in Malaysia prior to this. Generally, virulent vtaA was detected for all isolates, except for one, which displayed a nonvirulent vtaA. A phylogenetic analysis of the OmpP2 gene revealed that the majority of Malaysian isolates were clustered into genotype 1, which could be further divided into Ia and Ib, while only one isolate was clustered into genotype 2.
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spelling pubmed-99668542023-02-26 Molecular Characterization and Phylogenetic Analysis of Outer membrane protein P2 (OmpP2) of Glaesserella (Haemophilus) parasuis Isolates in Central State of Peninsular Malaysia Lee, Chee Yien Ong, Hui Xin Tan, Chew Yee Low, Suet Ee Phang, Lai Yee Lai, Jyhmirn Ooi, Peck Toung Fong, Michelle Wai Cheng Pathogens Article Glaesserella (Haemophilus) parasuis, the etiological agent of Glässer’s disease, is an economically significant pathogen commonly associated with serofibrinous polyserositis, arthritis, fibrinous bronchopneumonia and/or meningitis. This study is the first attempt to molecularly characterize and provide a detailed overview of the genetic variants of G. parasuis present in Malaysia, in reference to its serotype, virulence-associated trimeric autotransporters (vtaA) gene and outer membrane protein P2 (OmpP2) gene. The G. parasuis isolates (n = 11) from clinically sick field samples collected from two major pig producing states (Selangor and Perak) were selected for analysis. Upon multiplex PCR, the majority of the isolates (eight out of 11) were identified to be serotype 5 or 12, and interestingly, serotypes 3, 8 and 15 were also detected, which had never been reported in Malaysia prior to this. Generally, virulent vtaA was detected for all isolates, except for one, which displayed a nonvirulent vtaA. A phylogenetic analysis of the OmpP2 gene revealed that the majority of Malaysian isolates were clustered into genotype 1, which could be further divided into Ia and Ib, while only one isolate was clustered into genotype 2. MDPI 2023-02-12 /pmc/articles/PMC9966854/ /pubmed/36839580 http://dx.doi.org/10.3390/pathogens12020308 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Chee Yien
Ong, Hui Xin
Tan, Chew Yee
Low, Suet Ee
Phang, Lai Yee
Lai, Jyhmirn
Ooi, Peck Toung
Fong, Michelle Wai Cheng
Molecular Characterization and Phylogenetic Analysis of Outer membrane protein P2 (OmpP2) of Glaesserella (Haemophilus) parasuis Isolates in Central State of Peninsular Malaysia
title Molecular Characterization and Phylogenetic Analysis of Outer membrane protein P2 (OmpP2) of Glaesserella (Haemophilus) parasuis Isolates in Central State of Peninsular Malaysia
title_full Molecular Characterization and Phylogenetic Analysis of Outer membrane protein P2 (OmpP2) of Glaesserella (Haemophilus) parasuis Isolates in Central State of Peninsular Malaysia
title_fullStr Molecular Characterization and Phylogenetic Analysis of Outer membrane protein P2 (OmpP2) of Glaesserella (Haemophilus) parasuis Isolates in Central State of Peninsular Malaysia
title_full_unstemmed Molecular Characterization and Phylogenetic Analysis of Outer membrane protein P2 (OmpP2) of Glaesserella (Haemophilus) parasuis Isolates in Central State of Peninsular Malaysia
title_short Molecular Characterization and Phylogenetic Analysis of Outer membrane protein P2 (OmpP2) of Glaesserella (Haemophilus) parasuis Isolates in Central State of Peninsular Malaysia
title_sort molecular characterization and phylogenetic analysis of outer membrane protein p2 (ompp2) of glaesserella (haemophilus) parasuis isolates in central state of peninsular malaysia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966854/
https://www.ncbi.nlm.nih.gov/pubmed/36839580
http://dx.doi.org/10.3390/pathogens12020308
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