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Nuclear High Mobility Group A2 (HMGA2) Interactome Revealed by Biotin Proximity Labeling

The non-histone chromatin binding protein High Mobility Group AT-hook protein 2 (HMGA2) has important functions in chromatin remodeling, and genome maintenance and protection. Expression of HMGA2 is highest in embryonic stem cells, declines during cell differentiation and cell aging, but it is re-ex...

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Autores principales: Gaudreau-Lapierre, Antoine, Klonisch, Thomas, Nicolas, Hannah, Thanasupawat, Thatchawan, Trinkle-Mulcahy, Laura, Hombach-Klonisch, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966875/
https://www.ncbi.nlm.nih.gov/pubmed/36835656
http://dx.doi.org/10.3390/ijms24044246
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author Gaudreau-Lapierre, Antoine
Klonisch, Thomas
Nicolas, Hannah
Thanasupawat, Thatchawan
Trinkle-Mulcahy, Laura
Hombach-Klonisch, Sabine
author_facet Gaudreau-Lapierre, Antoine
Klonisch, Thomas
Nicolas, Hannah
Thanasupawat, Thatchawan
Trinkle-Mulcahy, Laura
Hombach-Klonisch, Sabine
author_sort Gaudreau-Lapierre, Antoine
collection PubMed
description The non-histone chromatin binding protein High Mobility Group AT-hook protein 2 (HMGA2) has important functions in chromatin remodeling, and genome maintenance and protection. Expression of HMGA2 is highest in embryonic stem cells, declines during cell differentiation and cell aging, but it is re-expressed in some cancers, where high HMGA2 expression frequently coincides with a poor prognosis. The nuclear functions of HMGA2 cannot be explained by binding to chromatin alone but involve complex interactions with other proteins that are incompletely understood. The present study used biotin proximity labeling, followed by proteomic analysis, to identify the nuclear interaction partners of HMGA2. We tested two different biotin ligase HMGA2 constructs (BioID2 and miniTurbo) with similar results, and identified known and new HMGA2 interaction partners, with functionalities mainly in chromatin biology. These HMGA2 biotin ligase fusion constructs offer exciting new possibilities for interactome discovery research, enabling the monitoring of nuclear HMGA2 interactomes during drug treatments.
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spelling pubmed-99668752023-02-26 Nuclear High Mobility Group A2 (HMGA2) Interactome Revealed by Biotin Proximity Labeling Gaudreau-Lapierre, Antoine Klonisch, Thomas Nicolas, Hannah Thanasupawat, Thatchawan Trinkle-Mulcahy, Laura Hombach-Klonisch, Sabine Int J Mol Sci Article The non-histone chromatin binding protein High Mobility Group AT-hook protein 2 (HMGA2) has important functions in chromatin remodeling, and genome maintenance and protection. Expression of HMGA2 is highest in embryonic stem cells, declines during cell differentiation and cell aging, but it is re-expressed in some cancers, where high HMGA2 expression frequently coincides with a poor prognosis. The nuclear functions of HMGA2 cannot be explained by binding to chromatin alone but involve complex interactions with other proteins that are incompletely understood. The present study used biotin proximity labeling, followed by proteomic analysis, to identify the nuclear interaction partners of HMGA2. We tested two different biotin ligase HMGA2 constructs (BioID2 and miniTurbo) with similar results, and identified known and new HMGA2 interaction partners, with functionalities mainly in chromatin biology. These HMGA2 biotin ligase fusion constructs offer exciting new possibilities for interactome discovery research, enabling the monitoring of nuclear HMGA2 interactomes during drug treatments. MDPI 2023-02-20 /pmc/articles/PMC9966875/ /pubmed/36835656 http://dx.doi.org/10.3390/ijms24044246 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gaudreau-Lapierre, Antoine
Klonisch, Thomas
Nicolas, Hannah
Thanasupawat, Thatchawan
Trinkle-Mulcahy, Laura
Hombach-Klonisch, Sabine
Nuclear High Mobility Group A2 (HMGA2) Interactome Revealed by Biotin Proximity Labeling
title Nuclear High Mobility Group A2 (HMGA2) Interactome Revealed by Biotin Proximity Labeling
title_full Nuclear High Mobility Group A2 (HMGA2) Interactome Revealed by Biotin Proximity Labeling
title_fullStr Nuclear High Mobility Group A2 (HMGA2) Interactome Revealed by Biotin Proximity Labeling
title_full_unstemmed Nuclear High Mobility Group A2 (HMGA2) Interactome Revealed by Biotin Proximity Labeling
title_short Nuclear High Mobility Group A2 (HMGA2) Interactome Revealed by Biotin Proximity Labeling
title_sort nuclear high mobility group a2 (hmga2) interactome revealed by biotin proximity labeling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966875/
https://www.ncbi.nlm.nih.gov/pubmed/36835656
http://dx.doi.org/10.3390/ijms24044246
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