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Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication
Ubiquitination and deubiquitination processes are widely involved in modulating the function, activity, localization, and stability of multiple cellular proteins regulating almost every aspect of cellular function. Several virus families have been shown to exploit the cellular ubiquitin-conjugating...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966916/ https://www.ncbi.nlm.nih.gov/pubmed/36851696 http://dx.doi.org/10.3390/v15020481 |
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author | López, Lady S. Calvo, Eliana P. Castellanos, Jaime E. |
author_facet | López, Lady S. Calvo, Eliana P. Castellanos, Jaime E. |
author_sort | López, Lady S. |
collection | PubMed |
description | Ubiquitination and deubiquitination processes are widely involved in modulating the function, activity, localization, and stability of multiple cellular proteins regulating almost every aspect of cellular function. Several virus families have been shown to exploit the cellular ubiquitin-conjugating system to achieve a productive infection: enter the cell, promote genome replication, or assemble and release viral progeny. In this study, we analyzed the role of deubiquitinating enzymes (DUBs) during chikungunya virus (CHIKV) infection. HEK293T, Vero-E6, and Huh-7 cells were treated with two DUB inhibitors (PR619 or WP1130). Then, infected cells were evaluated by flow cytometry, and viral progeny was quantified using the plaque assay method. The changes in viral proteins and viral RNA were analyzed using Western blotting and RT-qPCR, respectively. Results indicate that treatment with DUB inhibitors impairs CHIKV replication due to significant protein and viral RNA synthesis deregulation. Therefore, DUB activity may be a pharmacological target for blocking CHIKV infection. |
format | Online Article Text |
id | pubmed-9966916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99669162023-02-26 Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication López, Lady S. Calvo, Eliana P. Castellanos, Jaime E. Viruses Article Ubiquitination and deubiquitination processes are widely involved in modulating the function, activity, localization, and stability of multiple cellular proteins regulating almost every aspect of cellular function. Several virus families have been shown to exploit the cellular ubiquitin-conjugating system to achieve a productive infection: enter the cell, promote genome replication, or assemble and release viral progeny. In this study, we analyzed the role of deubiquitinating enzymes (DUBs) during chikungunya virus (CHIKV) infection. HEK293T, Vero-E6, and Huh-7 cells were treated with two DUB inhibitors (PR619 or WP1130). Then, infected cells were evaluated by flow cytometry, and viral progeny was quantified using the plaque assay method. The changes in viral proteins and viral RNA were analyzed using Western blotting and RT-qPCR, respectively. Results indicate that treatment with DUB inhibitors impairs CHIKV replication due to significant protein and viral RNA synthesis deregulation. Therefore, DUB activity may be a pharmacological target for blocking CHIKV infection. MDPI 2023-02-09 /pmc/articles/PMC9966916/ /pubmed/36851696 http://dx.doi.org/10.3390/v15020481 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article López, Lady S. Calvo, Eliana P. Castellanos, Jaime E. Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication |
title | Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication |
title_full | Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication |
title_fullStr | Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication |
title_full_unstemmed | Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication |
title_short | Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication |
title_sort | deubiquitinating enzyme inhibitors block chikungunya virus replication |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9966916/ https://www.ncbi.nlm.nih.gov/pubmed/36851696 http://dx.doi.org/10.3390/v15020481 |
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