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Seven Years of Monitoring Susceptibility to Cry1Ab and Cry1F in Asian Corn Borer
Resistance monitoring in the Asian corn borer, Ostrinia furnacalis, is necessary to accommodate the commercial introduction and stewardship of Bt maize in China. The susceptibility of 56 O. furnacalis field populations, collected between 2015 and 2021 from the corn belt regions of China, to Cry1Ab a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967349/ https://www.ncbi.nlm.nih.gov/pubmed/36828451 http://dx.doi.org/10.3390/toxins15020137 |
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author | Wang, Yueqin Zhao, Wenlu Han, Shuang Wang, Lianxia Chang, Xue Liu, Kaiqiang Quan, Yudong Wang, Zhenying He, Kanglai |
author_facet | Wang, Yueqin Zhao, Wenlu Han, Shuang Wang, Lianxia Chang, Xue Liu, Kaiqiang Quan, Yudong Wang, Zhenying He, Kanglai |
author_sort | Wang, Yueqin |
collection | PubMed |
description | Resistance monitoring in the Asian corn borer, Ostrinia furnacalis, is necessary to accommodate the commercial introduction and stewardship of Bt maize in China. The susceptibility of 56 O. furnacalis field populations, collected between 2015 and 2021 from the corn belt regions of China, to Cry1Ab and Cry1F toxins was determined. Neonate larvae (within 12 h after hatching) were placed on the surface of semi-artificial agar-free diet incorporating a series of concentrations of purified toxins, and mortality was evaluated after 7d. The median lethal concentration (LC(50)) values of Cry1Ab and Cry1F were 0.05 to 0.37 µg/g (protein/diet) and 0.10 to 1.22 µg/g, respectively. Although interpopulation variation in susceptibility to the toxins was observed, the magnitude of the differences was 5.8-fold and 8.3-fold for Cry1Ab and Cry1F, respectively. These results suggested that the observed susceptibility differences reflect natural geographical variation in response and not variation caused by prior exposure to selection pressures. Therefore, the O. furnacalis populations were apparently still susceptible to Cry1Ab and Cry1F across their range within China. The monitoring data established here will serve as a comparative reference for early warning signs of field-evolved resistance after the cultivation of Bt maize in China. |
format | Online Article Text |
id | pubmed-9967349 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99673492023-02-26 Seven Years of Monitoring Susceptibility to Cry1Ab and Cry1F in Asian Corn Borer Wang, Yueqin Zhao, Wenlu Han, Shuang Wang, Lianxia Chang, Xue Liu, Kaiqiang Quan, Yudong Wang, Zhenying He, Kanglai Toxins (Basel) Article Resistance monitoring in the Asian corn borer, Ostrinia furnacalis, is necessary to accommodate the commercial introduction and stewardship of Bt maize in China. The susceptibility of 56 O. furnacalis field populations, collected between 2015 and 2021 from the corn belt regions of China, to Cry1Ab and Cry1F toxins was determined. Neonate larvae (within 12 h after hatching) were placed on the surface of semi-artificial agar-free diet incorporating a series of concentrations of purified toxins, and mortality was evaluated after 7d. The median lethal concentration (LC(50)) values of Cry1Ab and Cry1F were 0.05 to 0.37 µg/g (protein/diet) and 0.10 to 1.22 µg/g, respectively. Although interpopulation variation in susceptibility to the toxins was observed, the magnitude of the differences was 5.8-fold and 8.3-fold for Cry1Ab and Cry1F, respectively. These results suggested that the observed susceptibility differences reflect natural geographical variation in response and not variation caused by prior exposure to selection pressures. Therefore, the O. furnacalis populations were apparently still susceptible to Cry1Ab and Cry1F across their range within China. The monitoring data established here will serve as a comparative reference for early warning signs of field-evolved resistance after the cultivation of Bt maize in China. MDPI 2023-02-07 /pmc/articles/PMC9967349/ /pubmed/36828451 http://dx.doi.org/10.3390/toxins15020137 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Yueqin Zhao, Wenlu Han, Shuang Wang, Lianxia Chang, Xue Liu, Kaiqiang Quan, Yudong Wang, Zhenying He, Kanglai Seven Years of Monitoring Susceptibility to Cry1Ab and Cry1F in Asian Corn Borer |
title | Seven Years of Monitoring Susceptibility to Cry1Ab and Cry1F in Asian Corn Borer |
title_full | Seven Years of Monitoring Susceptibility to Cry1Ab and Cry1F in Asian Corn Borer |
title_fullStr | Seven Years of Monitoring Susceptibility to Cry1Ab and Cry1F in Asian Corn Borer |
title_full_unstemmed | Seven Years of Monitoring Susceptibility to Cry1Ab and Cry1F in Asian Corn Borer |
title_short | Seven Years of Monitoring Susceptibility to Cry1Ab and Cry1F in Asian Corn Borer |
title_sort | seven years of monitoring susceptibility to cry1ab and cry1f in asian corn borer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967349/ https://www.ncbi.nlm.nih.gov/pubmed/36828451 http://dx.doi.org/10.3390/toxins15020137 |
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