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The Non-JAZ TIFY Protein TIFY8 of Arabidopsis thaliana Interacts with the HD-ZIP III Transcription Factor REVOLUTA and Regulates Leaf Senescence

The HD-ZIP III transcription factor REVOLUTA (REV) is involved in early leaf development, as well as in leaf senescence. REV directly binds to the promoters of senescence-associated genes, including the central regulator WRKY53. As this direct regulation appears to be restricted to senescence, we ai...

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Autores principales: Andrade Galan, Ana Gabriela, Doll, Jasmin, Saile, Svenja Corina, Wünsch, Marieluise, von Roepenack-Lahaye, Edda, Pauwels, Laurens, Goossens, Alain, Bresson, Justine, Zentgraf, Ulrike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967580/
https://www.ncbi.nlm.nih.gov/pubmed/36834490
http://dx.doi.org/10.3390/ijms24043079
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author Andrade Galan, Ana Gabriela
Doll, Jasmin
Saile, Svenja Corina
Wünsch, Marieluise
von Roepenack-Lahaye, Edda
Pauwels, Laurens
Goossens, Alain
Bresson, Justine
Zentgraf, Ulrike
author_facet Andrade Galan, Ana Gabriela
Doll, Jasmin
Saile, Svenja Corina
Wünsch, Marieluise
von Roepenack-Lahaye, Edda
Pauwels, Laurens
Goossens, Alain
Bresson, Justine
Zentgraf, Ulrike
author_sort Andrade Galan, Ana Gabriela
collection PubMed
description The HD-ZIP III transcription factor REVOLUTA (REV) is involved in early leaf development, as well as in leaf senescence. REV directly binds to the promoters of senescence-associated genes, including the central regulator WRKY53. As this direct regulation appears to be restricted to senescence, we aimed to characterize protein-interaction partners of REV which could mediate this senescence-specificity. The interaction between REV and the TIFY family member TIFY8 was confirmed by yeast two-hybrid assays, as well as by bimolecular fluorescence complementation in planta. This interaction inhibited REV’s function as an activator of WRKY53 expression. Mutation or overexpression of TIFY8 accelerated or delayed senescence, respectively, but did not significantly alter early leaf development. Jasmonic acid (JA) had only a limited effect on TIFY8 expression or function; however, REV appears to be under the control of JA signaling. Accordingly, REV also interacted with many other members of the TIFY family, namely the PEAPODs and several JAZ proteins in the yeast system, which could potentially mediate the JA-response. Therefore, REV appears to be under the control of the TIFY family in two different ways: a JA-independent way through TIFY8, which controls REV function in senescence, and a JA-dependent way through PEAPODs and JAZ proteins.
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spelling pubmed-99675802023-02-27 The Non-JAZ TIFY Protein TIFY8 of Arabidopsis thaliana Interacts with the HD-ZIP III Transcription Factor REVOLUTA and Regulates Leaf Senescence Andrade Galan, Ana Gabriela Doll, Jasmin Saile, Svenja Corina Wünsch, Marieluise von Roepenack-Lahaye, Edda Pauwels, Laurens Goossens, Alain Bresson, Justine Zentgraf, Ulrike Int J Mol Sci Article The HD-ZIP III transcription factor REVOLUTA (REV) is involved in early leaf development, as well as in leaf senescence. REV directly binds to the promoters of senescence-associated genes, including the central regulator WRKY53. As this direct regulation appears to be restricted to senescence, we aimed to characterize protein-interaction partners of REV which could mediate this senescence-specificity. The interaction between REV and the TIFY family member TIFY8 was confirmed by yeast two-hybrid assays, as well as by bimolecular fluorescence complementation in planta. This interaction inhibited REV’s function as an activator of WRKY53 expression. Mutation or overexpression of TIFY8 accelerated or delayed senescence, respectively, but did not significantly alter early leaf development. Jasmonic acid (JA) had only a limited effect on TIFY8 expression or function; however, REV appears to be under the control of JA signaling. Accordingly, REV also interacted with many other members of the TIFY family, namely the PEAPODs and several JAZ proteins in the yeast system, which could potentially mediate the JA-response. Therefore, REV appears to be under the control of the TIFY family in two different ways: a JA-independent way through TIFY8, which controls REV function in senescence, and a JA-dependent way through PEAPODs and JAZ proteins. MDPI 2023-02-04 /pmc/articles/PMC9967580/ /pubmed/36834490 http://dx.doi.org/10.3390/ijms24043079 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Andrade Galan, Ana Gabriela
Doll, Jasmin
Saile, Svenja Corina
Wünsch, Marieluise
von Roepenack-Lahaye, Edda
Pauwels, Laurens
Goossens, Alain
Bresson, Justine
Zentgraf, Ulrike
The Non-JAZ TIFY Protein TIFY8 of Arabidopsis thaliana Interacts with the HD-ZIP III Transcription Factor REVOLUTA and Regulates Leaf Senescence
title The Non-JAZ TIFY Protein TIFY8 of Arabidopsis thaliana Interacts with the HD-ZIP III Transcription Factor REVOLUTA and Regulates Leaf Senescence
title_full The Non-JAZ TIFY Protein TIFY8 of Arabidopsis thaliana Interacts with the HD-ZIP III Transcription Factor REVOLUTA and Regulates Leaf Senescence
title_fullStr The Non-JAZ TIFY Protein TIFY8 of Arabidopsis thaliana Interacts with the HD-ZIP III Transcription Factor REVOLUTA and Regulates Leaf Senescence
title_full_unstemmed The Non-JAZ TIFY Protein TIFY8 of Arabidopsis thaliana Interacts with the HD-ZIP III Transcription Factor REVOLUTA and Regulates Leaf Senescence
title_short The Non-JAZ TIFY Protein TIFY8 of Arabidopsis thaliana Interacts with the HD-ZIP III Transcription Factor REVOLUTA and Regulates Leaf Senescence
title_sort non-jaz tify protein tify8 of arabidopsis thaliana interacts with the hd-zip iii transcription factor revoluta and regulates leaf senescence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967580/
https://www.ncbi.nlm.nih.gov/pubmed/36834490
http://dx.doi.org/10.3390/ijms24043079
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