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Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle
BACKGROUND AND AIM: Bacterial and viral infections affect the welfare of animals and lead to large economic losses in dairy cattle breeding due to decreased productive indicators and increased culling rates. In modern dairy farming, farmers are looking for effective solutions to prevent and minimize...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967724/ https://www.ncbi.nlm.nih.gov/pubmed/36855359 http://dx.doi.org/10.14202/vetworld.2023.161-167 |
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author | Beishova, Indira Saltanovna Belaya, Alena Valentinovna Yuldashbayev, Yusupzhan Artykovich Chuzhebayeva, Gulzhagan Dzhambulovna Ulyanov, Vadim Alexandrovich Ulyanova, Tatyana Vladimirovna Kovalchuk, Alexandr Mikhailovich Kuzhebayeva, Ulbolsyn Zhangaziyevna Namet, Aidar Myrzahmetuly |
author_facet | Beishova, Indira Saltanovna Belaya, Alena Valentinovna Yuldashbayev, Yusupzhan Artykovich Chuzhebayeva, Gulzhagan Dzhambulovna Ulyanov, Vadim Alexandrovich Ulyanova, Tatyana Vladimirovna Kovalchuk, Alexandr Mikhailovich Kuzhebayeva, Ulbolsyn Zhangaziyevna Namet, Aidar Myrzahmetuly |
author_sort | Beishova, Indira Saltanovna |
collection | PubMed |
description | BACKGROUND AND AIM: Bacterial and viral infections affect the welfare of animals and lead to large economic losses in dairy cattle breeding due to decreased productive indicators and increased culling rates. In modern dairy farming, farmers are looking for effective solutions to prevent and minimize infectious disease risks. To this end, the most relevant study field is the search for gene sites that impact production and health. This study aimed to determine the nature of the distribution of the relative frequencies of alleles and genotypes of polymorphic prolactin (PRL) and nitric oxide synthase (NOS2) in Holstein cows and identify the relationship of these genes with resistance to mastitis and bovine leukemia. MATERIALS AND METHODS: For this study, we chose cows because infectious diseases affect the amount of lactation and milk quality. Holstein cattle with mastitis and bovine leukemia were selected. Animal genotypes were determined by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products. The results were analyzed using a nonparametric statistical method using Microsoft Excel 2010 and Statistica 6.0. RESULTS: In healthy animals, 94 genotypes were identified for both genes under study. For bPRL, bPRL-RsaI(AA) (72) was the most common genotype and bPRL-RsaI(BB) (4) the least; for NOS2, bNOS2-HinfI(AB) (47) was the most common genotype and bNOS2-HinfI(AA) the least (21). In animals with leukemia, 34 genotypes were identified. For PRL, bPRL-RsaI(AA) (25) was the most common genotype and bPRL-RsaI(BB) (2) the least; for NOS2, bNOS2-HinfI(BB) (17) was the most common genotype and bNOS2-HinfI(AA) (3) the least. In animals with mastitis, 67 genotypes were identified. For PRL, bPRL-RsaI(AA) (43) was the most common genotype and bPRL-RsaI(BB) (6) the least; for NOS2, bNOS2-HinfI(BB) (31) was the most common genotype and bNOS2-HinfI(AA) (7) the least. The distribution of genotypes of polymorphic bPRL and bNOS2 generally coincides, and bPRL-RsaI(BB) is the most common genotype. In groups of sick animals, the number of bNOS2-HinfI(AA) homozygotes was lower than that of the control group. In particular, the proportion of animals with the bNOS2-HinfI(AA) genotype with bovine leukemia was 8.7% and with mastitis was 10.3% compared with 22.4% in healthy animals. These data support the possible association of the bNOS2-HinfI(AA) genotype with resistance to infection. The frequency of the bPRL-RsaI(B) allele was higher in groups of sick animals. This allele is associated with increased milk productivity, suggesting that highly productive animals are less resistant to the incidence of viral bovine leukemia and mastitis of bacterial etiology. CONCLUSION: DNA amplification of Holstein cattle for the polymorphic regions of PRL and NOS2 using the PCR-RFLP method revealed a possible connection between the distribution of relative allele frequencies of bPRL and bNOS2 and resistance to viral and bacterial infections. Thus, in groups of sick animals, the frequency of bPRL-RsaI(BB), associated with increased milk production compared with the theoretically calculated equilibrium value was higher and the number of homozygotes bNOS2-HinfI(AA) was lower than in the control group. In conclusion, animals with increased milk production were more prone to diseases, such as mastitis and bovine leukemia. |
format | Online Article Text |
id | pubmed-9967724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-99677242023-02-27 Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle Beishova, Indira Saltanovna Belaya, Alena Valentinovna Yuldashbayev, Yusupzhan Artykovich Chuzhebayeva, Gulzhagan Dzhambulovna Ulyanov, Vadim Alexandrovich Ulyanova, Tatyana Vladimirovna Kovalchuk, Alexandr Mikhailovich Kuzhebayeva, Ulbolsyn Zhangaziyevna Namet, Aidar Myrzahmetuly Vet World Research Article BACKGROUND AND AIM: Bacterial and viral infections affect the welfare of animals and lead to large economic losses in dairy cattle breeding due to decreased productive indicators and increased culling rates. In modern dairy farming, farmers are looking for effective solutions to prevent and minimize infectious disease risks. To this end, the most relevant study field is the search for gene sites that impact production and health. This study aimed to determine the nature of the distribution of the relative frequencies of alleles and genotypes of polymorphic prolactin (PRL) and nitric oxide synthase (NOS2) in Holstein cows and identify the relationship of these genes with resistance to mastitis and bovine leukemia. MATERIALS AND METHODS: For this study, we chose cows because infectious diseases affect the amount of lactation and milk quality. Holstein cattle with mastitis and bovine leukemia were selected. Animal genotypes were determined by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products. The results were analyzed using a nonparametric statistical method using Microsoft Excel 2010 and Statistica 6.0. RESULTS: In healthy animals, 94 genotypes were identified for both genes under study. For bPRL, bPRL-RsaI(AA) (72) was the most common genotype and bPRL-RsaI(BB) (4) the least; for NOS2, bNOS2-HinfI(AB) (47) was the most common genotype and bNOS2-HinfI(AA) the least (21). In animals with leukemia, 34 genotypes were identified. For PRL, bPRL-RsaI(AA) (25) was the most common genotype and bPRL-RsaI(BB) (2) the least; for NOS2, bNOS2-HinfI(BB) (17) was the most common genotype and bNOS2-HinfI(AA) (3) the least. In animals with mastitis, 67 genotypes were identified. For PRL, bPRL-RsaI(AA) (43) was the most common genotype and bPRL-RsaI(BB) (6) the least; for NOS2, bNOS2-HinfI(BB) (31) was the most common genotype and bNOS2-HinfI(AA) (7) the least. The distribution of genotypes of polymorphic bPRL and bNOS2 generally coincides, and bPRL-RsaI(BB) is the most common genotype. In groups of sick animals, the number of bNOS2-HinfI(AA) homozygotes was lower than that of the control group. In particular, the proportion of animals with the bNOS2-HinfI(AA) genotype with bovine leukemia was 8.7% and with mastitis was 10.3% compared with 22.4% in healthy animals. These data support the possible association of the bNOS2-HinfI(AA) genotype with resistance to infection. The frequency of the bPRL-RsaI(B) allele was higher in groups of sick animals. This allele is associated with increased milk productivity, suggesting that highly productive animals are less resistant to the incidence of viral bovine leukemia and mastitis of bacterial etiology. CONCLUSION: DNA amplification of Holstein cattle for the polymorphic regions of PRL and NOS2 using the PCR-RFLP method revealed a possible connection between the distribution of relative allele frequencies of bPRL and bNOS2 and resistance to viral and bacterial infections. Thus, in groups of sick animals, the frequency of bPRL-RsaI(BB), associated with increased milk production compared with the theoretically calculated equilibrium value was higher and the number of homozygotes bNOS2-HinfI(AA) was lower than in the control group. In conclusion, animals with increased milk production were more prone to diseases, such as mastitis and bovine leukemia. Veterinary World 2023-01 2023-01-27 /pmc/articles/PMC9967724/ /pubmed/36855359 http://dx.doi.org/10.14202/vetworld.2023.161-167 Text en Copyright: © Beishova, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Beishova, Indira Saltanovna Belaya, Alena Valentinovna Yuldashbayev, Yusupzhan Artykovich Chuzhebayeva, Gulzhagan Dzhambulovna Ulyanov, Vadim Alexandrovich Ulyanova, Tatyana Vladimirovna Kovalchuk, Alexandr Mikhailovich Kuzhebayeva, Ulbolsyn Zhangaziyevna Namet, Aidar Myrzahmetuly Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle |
title | Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle |
title_full | Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle |
title_fullStr | Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle |
title_full_unstemmed | Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle |
title_short | Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle |
title_sort | genetic polymorphism of prolactin and nitric oxide synthase in holstein cattle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967724/ https://www.ncbi.nlm.nih.gov/pubmed/36855359 http://dx.doi.org/10.14202/vetworld.2023.161-167 |
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