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Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration
Microfluidic preconcentration enables the collection or extraction of low-abundance analytes at specific locations. It has attracted considerable attention as an essential technology in bioengineering, particularly for detection and diagnosis. Herein, we investigated the key parameters in the precon...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967745/ https://www.ncbi.nlm.nih.gov/pubmed/36838098 http://dx.doi.org/10.3390/mi14020398 |
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author | Kim, Minsung Kim, Bumjoo |
author_facet | Kim, Minsung Kim, Bumjoo |
author_sort | Kim, Minsung |
collection | PubMed |
description | Microfluidic preconcentration enables the collection or extraction of low-abundance analytes at specific locations. It has attracted considerable attention as an essential technology in bioengineering, particularly for detection and diagnosis. Herein, we investigated the key parameters in the preconcentration of fluorescent dyes based on electrophoresis in a microfluidic electromembrane system. Commercial ion-exchange membrane (IEM)-integrated polydimethylsiloxane microfluidic devices were fabricated, and Alexa Fluor 488 and Rhodamine 6G were used as fluorescent dyes for sample preconcentration. Through experimental studies, the effect of the channel concentration ratio (CCR, concentration ratio of the main and buffer channels) on the performance of the sample preconcentration was studied. The results show that the preconcentration of the target sample occurs more effectively for a high CCR or high salt concentration of the main channel when the CCR is constant. We also demonstrate a phenomenon that the salt concentration in the electrolyte solution increases as the preconcentration progresses. Our results provide consolidated conditions for electrophoresis-based sample preconcentration in electromembrane systems. |
format | Online Article Text |
id | pubmed-9967745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99677452023-02-27 Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration Kim, Minsung Kim, Bumjoo Micromachines (Basel) Article Microfluidic preconcentration enables the collection or extraction of low-abundance analytes at specific locations. It has attracted considerable attention as an essential technology in bioengineering, particularly for detection and diagnosis. Herein, we investigated the key parameters in the preconcentration of fluorescent dyes based on electrophoresis in a microfluidic electromembrane system. Commercial ion-exchange membrane (IEM)-integrated polydimethylsiloxane microfluidic devices were fabricated, and Alexa Fluor 488 and Rhodamine 6G were used as fluorescent dyes for sample preconcentration. Through experimental studies, the effect of the channel concentration ratio (CCR, concentration ratio of the main and buffer channels) on the performance of the sample preconcentration was studied. The results show that the preconcentration of the target sample occurs more effectively for a high CCR or high salt concentration of the main channel when the CCR is constant. We also demonstrate a phenomenon that the salt concentration in the electrolyte solution increases as the preconcentration progresses. Our results provide consolidated conditions for electrophoresis-based sample preconcentration in electromembrane systems. MDPI 2023-02-06 /pmc/articles/PMC9967745/ /pubmed/36838098 http://dx.doi.org/10.3390/mi14020398 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Minsung Kim, Bumjoo Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration |
title | Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration |
title_full | Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration |
title_fullStr | Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration |
title_full_unstemmed | Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration |
title_short | Preconcentration of Fluorescent Dyes in Electromembrane Systems via Electrophoretic Migration |
title_sort | preconcentration of fluorescent dyes in electromembrane systems via electrophoretic migration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967745/ https://www.ncbi.nlm.nih.gov/pubmed/36838098 http://dx.doi.org/10.3390/mi14020398 |
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