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Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata
Sinningia bullata is a tuberous member of the flowering plant family Gesneriaceae. Prior to this work, the antibacterial, antioxidant, and cytotoxic properties of S. bullata were undetermined. Here, we prepared different extracts from the leaf, stem, and tuber of S. bullata and investigated their ph...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967939/ https://www.ncbi.nlm.nih.gov/pubmed/36840206 http://dx.doi.org/10.3390/plants12040859 |
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author | Chen, Pin-Jui Lin, En-Shyh Su, Hsin-Hui Huang, Cheng-Yang |
author_facet | Chen, Pin-Jui Lin, En-Shyh Su, Hsin-Hui Huang, Cheng-Yang |
author_sort | Chen, Pin-Jui |
collection | PubMed |
description | Sinningia bullata is a tuberous member of the flowering plant family Gesneriaceae. Prior to this work, the antibacterial, antioxidant, and cytotoxic properties of S. bullata were undetermined. Here, we prepared different extracts from the leaf, stem, and tuber of S. bullata and investigated their pharmacological activities. The leaf extract of S. bullata, obtained by 100% acetone (Sb-L-A), had the highest total flavonoid content, antioxidation capacity, and cytotoxic and antibacterial activities. Sb-L-A displayed a broad range of antibacterial activities against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The inhibition zones of Sb-L-A ranged from 8 to 30 mm and were in the following order: S. aureus > E. coli > P. aeruginosa. Incubation of B16F10 melanoma cells with Sb-L-A at a concentration of 80 μg/mL caused deaths at the rate of 96%, reduced migration by 100%, suppressed proliferation and colony formation by 99%, and induced apoptosis, which was observed in 96% of the B16F10 cells. In addition, the cytotoxic activities of Sb-L-A were synergistically enhanced when coacting with the antitumor drug epothilone B. Sb-L-A was also used to determine the cytotoxic effects against 4T1 mammary carcinoma cells. Sb-L-A of 60 μg/mL boosted the distribution of the G2 phase from 1.4% to 24.4% in the B16F10 cells. Accordingly, Sb-L-A might suppress melanoma cell proliferation by inducing G2 cell-cycle arrest. The most abundant compounds in Sb-L-A were identified using gas chromatography–mass spectrometry. Overall, the collective data in this study may indicate the pharmacological potentials of Sb-L-A for possible medical applications. |
format | Online Article Text |
id | pubmed-9967939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99679392023-02-27 Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata Chen, Pin-Jui Lin, En-Shyh Su, Hsin-Hui Huang, Cheng-Yang Plants (Basel) Article Sinningia bullata is a tuberous member of the flowering plant family Gesneriaceae. Prior to this work, the antibacterial, antioxidant, and cytotoxic properties of S. bullata were undetermined. Here, we prepared different extracts from the leaf, stem, and tuber of S. bullata and investigated their pharmacological activities. The leaf extract of S. bullata, obtained by 100% acetone (Sb-L-A), had the highest total flavonoid content, antioxidation capacity, and cytotoxic and antibacterial activities. Sb-L-A displayed a broad range of antibacterial activities against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The inhibition zones of Sb-L-A ranged from 8 to 30 mm and were in the following order: S. aureus > E. coli > P. aeruginosa. Incubation of B16F10 melanoma cells with Sb-L-A at a concentration of 80 μg/mL caused deaths at the rate of 96%, reduced migration by 100%, suppressed proliferation and colony formation by 99%, and induced apoptosis, which was observed in 96% of the B16F10 cells. In addition, the cytotoxic activities of Sb-L-A were synergistically enhanced when coacting with the antitumor drug epothilone B. Sb-L-A was also used to determine the cytotoxic effects against 4T1 mammary carcinoma cells. Sb-L-A of 60 μg/mL boosted the distribution of the G2 phase from 1.4% to 24.4% in the B16F10 cells. Accordingly, Sb-L-A might suppress melanoma cell proliferation by inducing G2 cell-cycle arrest. The most abundant compounds in Sb-L-A were identified using gas chromatography–mass spectrometry. Overall, the collective data in this study may indicate the pharmacological potentials of Sb-L-A for possible medical applications. MDPI 2023-02-14 /pmc/articles/PMC9967939/ /pubmed/36840206 http://dx.doi.org/10.3390/plants12040859 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Pin-Jui Lin, En-Shyh Su, Hsin-Hui Huang, Cheng-Yang Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata |
title | Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata |
title_full | Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata |
title_fullStr | Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata |
title_full_unstemmed | Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata |
title_short | Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata |
title_sort | cytotoxic, antibacterial, and antioxidant activities of the leaf extract of sinningia bullata |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967939/ https://www.ncbi.nlm.nih.gov/pubmed/36840206 http://dx.doi.org/10.3390/plants12040859 |
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