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Metabolite Profiling to Evaluate Metabolic Changes in Genetically Modified Protopanaxadiol-Enriched Rice
Event DS rice producing protopanaxadiol (PPD) has been previously developed by inserting Panax ginseng dammarenediol-II synthase gene (PgDDS) and PPD synthase gene (CYP716A47). We performed a gas chromatography–mass spectrometry (GC–MS)-based metabolomics of the DS rice to identify metabolic alterat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967978/ https://www.ncbi.nlm.nih.gov/pubmed/36840106 http://dx.doi.org/10.3390/plants12040758 |
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author | Sim, Ji-Eun Oh, Sung-Dug Kang, Kiyoon Shin, Yu-Mi Yun, Doh-Won Baek, So-Hyeon Choi, Yong-Eui Park, Sang-Un Kim, Jae-Kwang |
author_facet | Sim, Ji-Eun Oh, Sung-Dug Kang, Kiyoon Shin, Yu-Mi Yun, Doh-Won Baek, So-Hyeon Choi, Yong-Eui Park, Sang-Un Kim, Jae-Kwang |
author_sort | Sim, Ji-Eun |
collection | PubMed |
description | Event DS rice producing protopanaxadiol (PPD) has been previously developed by inserting Panax ginseng dammarenediol-II synthase gene (PgDDS) and PPD synthase gene (CYP716A47). We performed a gas chromatography–mass spectrometry (GC–MS)-based metabolomics of the DS rice to identify metabolic alterations as the effects of genetic engineering by measuring the contents of 65 metabolites in seeds and 63 metabolites in leaves. Multivariate analysis and one-way analysis of variance between DS and non-genetically modified (GM) rice showed that DS rice accumulated fewer tocotrienols, tocopherols, and phytosterols than non-GM rice. These results may be due to competition for the same precursors because PPDs in DS rice are synthesized from the same precursors as those of phytosterols. In addition, multivariate analysis of metabolic data from rice leaves revealed that composition differed by growth stage rather than genetic modifications. Our results demonstrate the potential of metabolomics for identifying metabolic alterations in response to genetic modifications. |
format | Online Article Text |
id | pubmed-9967978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99679782023-02-27 Metabolite Profiling to Evaluate Metabolic Changes in Genetically Modified Protopanaxadiol-Enriched Rice Sim, Ji-Eun Oh, Sung-Dug Kang, Kiyoon Shin, Yu-Mi Yun, Doh-Won Baek, So-Hyeon Choi, Yong-Eui Park, Sang-Un Kim, Jae-Kwang Plants (Basel) Article Event DS rice producing protopanaxadiol (PPD) has been previously developed by inserting Panax ginseng dammarenediol-II synthase gene (PgDDS) and PPD synthase gene (CYP716A47). We performed a gas chromatography–mass spectrometry (GC–MS)-based metabolomics of the DS rice to identify metabolic alterations as the effects of genetic engineering by measuring the contents of 65 metabolites in seeds and 63 metabolites in leaves. Multivariate analysis and one-way analysis of variance between DS and non-genetically modified (GM) rice showed that DS rice accumulated fewer tocotrienols, tocopherols, and phytosterols than non-GM rice. These results may be due to competition for the same precursors because PPDs in DS rice are synthesized from the same precursors as those of phytosterols. In addition, multivariate analysis of metabolic data from rice leaves revealed that composition differed by growth stage rather than genetic modifications. Our results demonstrate the potential of metabolomics for identifying metabolic alterations in response to genetic modifications. MDPI 2023-02-08 /pmc/articles/PMC9967978/ /pubmed/36840106 http://dx.doi.org/10.3390/plants12040758 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sim, Ji-Eun Oh, Sung-Dug Kang, Kiyoon Shin, Yu-Mi Yun, Doh-Won Baek, So-Hyeon Choi, Yong-Eui Park, Sang-Un Kim, Jae-Kwang Metabolite Profiling to Evaluate Metabolic Changes in Genetically Modified Protopanaxadiol-Enriched Rice |
title | Metabolite Profiling to Evaluate Metabolic Changes in Genetically Modified Protopanaxadiol-Enriched Rice |
title_full | Metabolite Profiling to Evaluate Metabolic Changes in Genetically Modified Protopanaxadiol-Enriched Rice |
title_fullStr | Metabolite Profiling to Evaluate Metabolic Changes in Genetically Modified Protopanaxadiol-Enriched Rice |
title_full_unstemmed | Metabolite Profiling to Evaluate Metabolic Changes in Genetically Modified Protopanaxadiol-Enriched Rice |
title_short | Metabolite Profiling to Evaluate Metabolic Changes in Genetically Modified Protopanaxadiol-Enriched Rice |
title_sort | metabolite profiling to evaluate metabolic changes in genetically modified protopanaxadiol-enriched rice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9967978/ https://www.ncbi.nlm.nih.gov/pubmed/36840106 http://dx.doi.org/10.3390/plants12040758 |
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