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Design, Physical Characterizations, and Biocompatibility of Cationic Solid Lipid Nanoparticles in HCT-116 and 16-HBE Cells: A Preliminary Study

In this study, pEGFP-LUC was used as a model plasmid and three distinct cationic lipids (dioleyloxy-propyl-trimethylammonium chloride [DOTMA], dioleoyl trimethylammonium propane [DOTAP], and cetylpyridinium chloride [CPC]) were tested along with PEG 5000, as a nonionic surfactant, to prepare glycery...

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Autores principales: Alamri, Ali, Alqahtani, Ali, Alqahtani, Taha, Al Fatease, Adel, Asiri, Saeed Ahmed, Gahtani, Reem M., Alnasser, Sulaiman Mohammed, Mohamed, Jamal Moideen Muthu, Menaa, Farid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968044/
https://www.ncbi.nlm.nih.gov/pubmed/36838699
http://dx.doi.org/10.3390/molecules28041711
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author Alamri, Ali
Alqahtani, Ali
Alqahtani, Taha
Al Fatease, Adel
Asiri, Saeed Ahmed
Gahtani, Reem M.
Alnasser, Sulaiman Mohammed
Mohamed, Jamal Moideen Muthu
Menaa, Farid
author_facet Alamri, Ali
Alqahtani, Ali
Alqahtani, Taha
Al Fatease, Adel
Asiri, Saeed Ahmed
Gahtani, Reem M.
Alnasser, Sulaiman Mohammed
Mohamed, Jamal Moideen Muthu
Menaa, Farid
author_sort Alamri, Ali
collection PubMed
description In this study, pEGFP-LUC was used as a model plasmid and three distinct cationic lipids (dioleyloxy-propyl-trimethylammonium chloride [DOTMA], dioleoyl trimethylammonium propane [DOTAP], and cetylpyridinium chloride [CPC]) were tested along with PEG 5000, as a nonionic surfactant, to prepare glyceryl monostearate (GMS)-based cationic solid lipid nanoparticles (cSLNs). Both the type and quantity of surfactant had an impact on the physicochemical characteristics of the cSLNs. Thermal analysis of the greater part of the endothermic peaks of the cSLNs revealed they were noticeably different from the individual pure compounds based on their zeta potential (ZP ranging from +17 to +56 mV) and particle size (PS ranging from 185 to 244 nm). The addition of cationic surfactants was required to produce nanoparticles (NPs) with a positive surface charge. This suggested that the surfactants and extensive entanglement of the lipid matrix GMS provided support for the behavioral diversity of the cSLNs and their capacity to interface with the plasmid DNA. Additionally, hemolytic assays were used to show that the cSLNs were biocompatible with the human colon cancer HCT-116 and human bronchial epithelial 16-HBE cell lines. The DOTMA 6-based cSLN was selected as the lead cSLN for further ex vivo and in vivo investigations. Taken together, these new findings might provide some guidance in selecting surfactants to prepare extremely efficient and non-toxic cSLN-based therapeutic delivery systems (e.g., gene therapy).
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spelling pubmed-99680442023-02-27 Design, Physical Characterizations, and Biocompatibility of Cationic Solid Lipid Nanoparticles in HCT-116 and 16-HBE Cells: A Preliminary Study Alamri, Ali Alqahtani, Ali Alqahtani, Taha Al Fatease, Adel Asiri, Saeed Ahmed Gahtani, Reem M. Alnasser, Sulaiman Mohammed Mohamed, Jamal Moideen Muthu Menaa, Farid Molecules Article In this study, pEGFP-LUC was used as a model plasmid and three distinct cationic lipids (dioleyloxy-propyl-trimethylammonium chloride [DOTMA], dioleoyl trimethylammonium propane [DOTAP], and cetylpyridinium chloride [CPC]) were tested along with PEG 5000, as a nonionic surfactant, to prepare glyceryl monostearate (GMS)-based cationic solid lipid nanoparticles (cSLNs). Both the type and quantity of surfactant had an impact on the physicochemical characteristics of the cSLNs. Thermal analysis of the greater part of the endothermic peaks of the cSLNs revealed they were noticeably different from the individual pure compounds based on their zeta potential (ZP ranging from +17 to +56 mV) and particle size (PS ranging from 185 to 244 nm). The addition of cationic surfactants was required to produce nanoparticles (NPs) with a positive surface charge. This suggested that the surfactants and extensive entanglement of the lipid matrix GMS provided support for the behavioral diversity of the cSLNs and their capacity to interface with the plasmid DNA. Additionally, hemolytic assays were used to show that the cSLNs were biocompatible with the human colon cancer HCT-116 and human bronchial epithelial 16-HBE cell lines. The DOTMA 6-based cSLN was selected as the lead cSLN for further ex vivo and in vivo investigations. Taken together, these new findings might provide some guidance in selecting surfactants to prepare extremely efficient and non-toxic cSLN-based therapeutic delivery systems (e.g., gene therapy). MDPI 2023-02-10 /pmc/articles/PMC9968044/ /pubmed/36838699 http://dx.doi.org/10.3390/molecules28041711 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alamri, Ali
Alqahtani, Ali
Alqahtani, Taha
Al Fatease, Adel
Asiri, Saeed Ahmed
Gahtani, Reem M.
Alnasser, Sulaiman Mohammed
Mohamed, Jamal Moideen Muthu
Menaa, Farid
Design, Physical Characterizations, and Biocompatibility of Cationic Solid Lipid Nanoparticles in HCT-116 and 16-HBE Cells: A Preliminary Study
title Design, Physical Characterizations, and Biocompatibility of Cationic Solid Lipid Nanoparticles in HCT-116 and 16-HBE Cells: A Preliminary Study
title_full Design, Physical Characterizations, and Biocompatibility of Cationic Solid Lipid Nanoparticles in HCT-116 and 16-HBE Cells: A Preliminary Study
title_fullStr Design, Physical Characterizations, and Biocompatibility of Cationic Solid Lipid Nanoparticles in HCT-116 and 16-HBE Cells: A Preliminary Study
title_full_unstemmed Design, Physical Characterizations, and Biocompatibility of Cationic Solid Lipid Nanoparticles in HCT-116 and 16-HBE Cells: A Preliminary Study
title_short Design, Physical Characterizations, and Biocompatibility of Cationic Solid Lipid Nanoparticles in HCT-116 and 16-HBE Cells: A Preliminary Study
title_sort design, physical characterizations, and biocompatibility of cationic solid lipid nanoparticles in hct-116 and 16-hbe cells: a preliminary study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968044/
https://www.ncbi.nlm.nih.gov/pubmed/36838699
http://dx.doi.org/10.3390/molecules28041711
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