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Protective Effect of Neutral Electrolyzed Saline on Gentamicin-Induced Nephrotoxicity: Evaluation of Histopathologic Parameters in a Murine Model

Background and Objectives: Gentamicin (GM) is a nephrotoxic aminoglycoside. Neutral electrolyzed saline (SES) is a compound with anti-inflammatory, antioxidant, and immunomodulatory properties. The objective of the present study was to evaluate whether kidney damage by GM can be prevented and/or rev...

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Autores principales: Aurelien-Cabezas, Nomely S., Paz-Michel, Brenda A., Jacinto-Cortes, Ivan, Delgado-Enciso, Osiris G., Montes-Galindo, Daniel A., Cabrera-Licona, Ariana, Zaizar-Fregoso, Sergio A., Paz-Garcia, Juan, Ceja-Espiritu, Gabriel, Melnikov, Valery, Guzman-Esquivel, Jose, Rodriguez-Sanchez, Iram P., Martinez-Fierro, Margarita L., Delgado-Enciso, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968118/
https://www.ncbi.nlm.nih.gov/pubmed/36837598
http://dx.doi.org/10.3390/medicina59020397
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author Aurelien-Cabezas, Nomely S.
Paz-Michel, Brenda A.
Jacinto-Cortes, Ivan
Delgado-Enciso, Osiris G.
Montes-Galindo, Daniel A.
Cabrera-Licona, Ariana
Zaizar-Fregoso, Sergio A.
Paz-Garcia, Juan
Ceja-Espiritu, Gabriel
Melnikov, Valery
Guzman-Esquivel, Jose
Rodriguez-Sanchez, Iram P.
Martinez-Fierro, Margarita L.
Delgado-Enciso, Ivan
author_facet Aurelien-Cabezas, Nomely S.
Paz-Michel, Brenda A.
Jacinto-Cortes, Ivan
Delgado-Enciso, Osiris G.
Montes-Galindo, Daniel A.
Cabrera-Licona, Ariana
Zaizar-Fregoso, Sergio A.
Paz-Garcia, Juan
Ceja-Espiritu, Gabriel
Melnikov, Valery
Guzman-Esquivel, Jose
Rodriguez-Sanchez, Iram P.
Martinez-Fierro, Margarita L.
Delgado-Enciso, Ivan
author_sort Aurelien-Cabezas, Nomely S.
collection PubMed
description Background and Objectives: Gentamicin (GM) is a nephrotoxic aminoglycoside. Neutral electrolyzed saline (SES) is a compound with anti-inflammatory, antioxidant, and immunomodulatory properties. The objective of the present study was to evaluate whether kidney damage by GM can be prevented and/or reversed through the administration of SES. Materials and Methods: The study was carried out as a prospective, single-blind, five-arm, parallel-group, randomized, preclinical trial. The nephrotoxicity model was established in male BALB/c mice by administering GM at a dose of 100 mg/kg/day intraperitoneally for 30 days, concomitantly administering (+) SES or placebo (physiologic saline solution), and then administering SES for another 30 days after the initial 30 days of GM plus SES or placebo. At the end of the test, the mice were euthanized, and renal tissues were evaluated histopathologically. Results: The GM + placebo group showed significant tubular injury, interstitial fibrosis, and increased interstitial infiltrate of inflammatory cells compared with the group without GM. Tubular injury and interstitial fibrosis were lower in the groups that received concomitant GM + SES compared with the GM + placebo group. SES administration for 30 days after the GM administration periods (GM + placebo and GM + SES for 30 days) did not reduce nephrotoxicity. Conclusions: Intraperitoneal administration of SES prevents gentamicin-induced histologic nephrotoxicity when administered concomitantly, but it cannot reverse the damage when administered later.
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spelling pubmed-99681182023-02-27 Protective Effect of Neutral Electrolyzed Saline on Gentamicin-Induced Nephrotoxicity: Evaluation of Histopathologic Parameters in a Murine Model Aurelien-Cabezas, Nomely S. Paz-Michel, Brenda A. Jacinto-Cortes, Ivan Delgado-Enciso, Osiris G. Montes-Galindo, Daniel A. Cabrera-Licona, Ariana Zaizar-Fregoso, Sergio A. Paz-Garcia, Juan Ceja-Espiritu, Gabriel Melnikov, Valery Guzman-Esquivel, Jose Rodriguez-Sanchez, Iram P. Martinez-Fierro, Margarita L. Delgado-Enciso, Ivan Medicina (Kaunas) Article Background and Objectives: Gentamicin (GM) is a nephrotoxic aminoglycoside. Neutral electrolyzed saline (SES) is a compound with anti-inflammatory, antioxidant, and immunomodulatory properties. The objective of the present study was to evaluate whether kidney damage by GM can be prevented and/or reversed through the administration of SES. Materials and Methods: The study was carried out as a prospective, single-blind, five-arm, parallel-group, randomized, preclinical trial. The nephrotoxicity model was established in male BALB/c mice by administering GM at a dose of 100 mg/kg/day intraperitoneally for 30 days, concomitantly administering (+) SES or placebo (physiologic saline solution), and then administering SES for another 30 days after the initial 30 days of GM plus SES or placebo. At the end of the test, the mice were euthanized, and renal tissues were evaluated histopathologically. Results: The GM + placebo group showed significant tubular injury, interstitial fibrosis, and increased interstitial infiltrate of inflammatory cells compared with the group without GM. Tubular injury and interstitial fibrosis were lower in the groups that received concomitant GM + SES compared with the GM + placebo group. SES administration for 30 days after the GM administration periods (GM + placebo and GM + SES for 30 days) did not reduce nephrotoxicity. Conclusions: Intraperitoneal administration of SES prevents gentamicin-induced histologic nephrotoxicity when administered concomitantly, but it cannot reverse the damage when administered later. MDPI 2023-02-17 /pmc/articles/PMC9968118/ /pubmed/36837598 http://dx.doi.org/10.3390/medicina59020397 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aurelien-Cabezas, Nomely S.
Paz-Michel, Brenda A.
Jacinto-Cortes, Ivan
Delgado-Enciso, Osiris G.
Montes-Galindo, Daniel A.
Cabrera-Licona, Ariana
Zaizar-Fregoso, Sergio A.
Paz-Garcia, Juan
Ceja-Espiritu, Gabriel
Melnikov, Valery
Guzman-Esquivel, Jose
Rodriguez-Sanchez, Iram P.
Martinez-Fierro, Margarita L.
Delgado-Enciso, Ivan
Protective Effect of Neutral Electrolyzed Saline on Gentamicin-Induced Nephrotoxicity: Evaluation of Histopathologic Parameters in a Murine Model
title Protective Effect of Neutral Electrolyzed Saline on Gentamicin-Induced Nephrotoxicity: Evaluation of Histopathologic Parameters in a Murine Model
title_full Protective Effect of Neutral Electrolyzed Saline on Gentamicin-Induced Nephrotoxicity: Evaluation of Histopathologic Parameters in a Murine Model
title_fullStr Protective Effect of Neutral Electrolyzed Saline on Gentamicin-Induced Nephrotoxicity: Evaluation of Histopathologic Parameters in a Murine Model
title_full_unstemmed Protective Effect of Neutral Electrolyzed Saline on Gentamicin-Induced Nephrotoxicity: Evaluation of Histopathologic Parameters in a Murine Model
title_short Protective Effect of Neutral Electrolyzed Saline on Gentamicin-Induced Nephrotoxicity: Evaluation of Histopathologic Parameters in a Murine Model
title_sort protective effect of neutral electrolyzed saline on gentamicin-induced nephrotoxicity: evaluation of histopathologic parameters in a murine model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968118/
https://www.ncbi.nlm.nih.gov/pubmed/36837598
http://dx.doi.org/10.3390/medicina59020397
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