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Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification
Plant cellulose microfibrils are increasingly employed to produce functional nanofibers and nanocrystals for biomaterials, but their catalytic formation and conversion mechanisms remain elusive. Here, we characterize length-reduced cellulose nanofibers assembly in situ accounting for the high densit...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968341/ https://www.ncbi.nlm.nih.gov/pubmed/36841862 http://dx.doi.org/10.1038/s41467-023-36856-8 |
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author | Zhang, Ran Hu, Zhen Wang, Yanting Hu, Huizhen Li, Fengcheng Li, Mi Ragauskas, Arthur Xia, Tao Han, Heyou Tang, Jingfeng Yu, Haizhong Xu, Bingqian Peng, Liangcai |
author_facet | Zhang, Ran Hu, Zhen Wang, Yanting Hu, Huizhen Li, Fengcheng Li, Mi Ragauskas, Arthur Xia, Tao Han, Heyou Tang, Jingfeng Yu, Haizhong Xu, Bingqian Peng, Liangcai |
author_sort | Zhang, Ran |
collection | PubMed |
description | Plant cellulose microfibrils are increasingly employed to produce functional nanofibers and nanocrystals for biomaterials, but their catalytic formation and conversion mechanisms remain elusive. Here, we characterize length-reduced cellulose nanofibers assembly in situ accounting for the high density of amorphous cellulose regions in the natural rice fragile culm 16 (Osfc16) mutant defective in cellulose biosynthesis using both classic and advanced atomic force microscopy (AFM) techniques equipped with a single-molecular recognition system. By employing individual types of cellulases, we observe efficient enzymatic catalysis modes in the mutant, due to amorphous and inner-broken cellulose chains elevated as breakpoints for initiating and completing cellulose hydrolyses into higher-yield fermentable sugars. Furthermore, effective chemical catalysis mode is examined in vitro for cellulose nanofibers conversion into nanocrystals with reduced dimensions. Our study addresses how plant cellulose substrates are digestible and convertible, revealing a strategy for precise engineering of cellulose substrates toward cost-effective biofuels and high-quality bioproducts. |
format | Online Article Text |
id | pubmed-9968341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-99683412023-02-27 Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification Zhang, Ran Hu, Zhen Wang, Yanting Hu, Huizhen Li, Fengcheng Li, Mi Ragauskas, Arthur Xia, Tao Han, Heyou Tang, Jingfeng Yu, Haizhong Xu, Bingqian Peng, Liangcai Nat Commun Article Plant cellulose microfibrils are increasingly employed to produce functional nanofibers and nanocrystals for biomaterials, but their catalytic formation and conversion mechanisms remain elusive. Here, we characterize length-reduced cellulose nanofibers assembly in situ accounting for the high density of amorphous cellulose regions in the natural rice fragile culm 16 (Osfc16) mutant defective in cellulose biosynthesis using both classic and advanced atomic force microscopy (AFM) techniques equipped with a single-molecular recognition system. By employing individual types of cellulases, we observe efficient enzymatic catalysis modes in the mutant, due to amorphous and inner-broken cellulose chains elevated as breakpoints for initiating and completing cellulose hydrolyses into higher-yield fermentable sugars. Furthermore, effective chemical catalysis mode is examined in vitro for cellulose nanofibers conversion into nanocrystals with reduced dimensions. Our study addresses how plant cellulose substrates are digestible and convertible, revealing a strategy for precise engineering of cellulose substrates toward cost-effective biofuels and high-quality bioproducts. Nature Publishing Group UK 2023-02-25 /pmc/articles/PMC9968341/ /pubmed/36841862 http://dx.doi.org/10.1038/s41467-023-36856-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhang, Ran Hu, Zhen Wang, Yanting Hu, Huizhen Li, Fengcheng Li, Mi Ragauskas, Arthur Xia, Tao Han, Heyou Tang, Jingfeng Yu, Haizhong Xu, Bingqian Peng, Liangcai Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification |
title | Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification |
title_full | Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification |
title_fullStr | Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification |
title_full_unstemmed | Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification |
title_short | Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification |
title_sort | single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968341/ https://www.ncbi.nlm.nih.gov/pubmed/36841862 http://dx.doi.org/10.1038/s41467-023-36856-8 |
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