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Physicochemical and nanomedicine applications of phyto-reduced erbium oxide (Er(2)O(3)) nanoparticles
Hyphaene thebaica fruits were used for the fabrication of spherical erbium oxide nanoparticles (HT-Er(2)O(3) NP(S)) using a one-step simple bioreduction process. XRD pattern revealed a highly crystalline and pure phase with crystallite size of ~ 7.5 nm, whereas, the W–H plot revealed crystallite siz...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968365/ https://www.ncbi.nlm.nih.gov/pubmed/36840788 http://dx.doi.org/10.1186/s13568-023-01527-w |
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author | Mohamed, Hamza Elsayed Ahmad Khalil, Ali Talha Hkiri, Khaoula Ayaz, Muhammad Abbasi, Jamil Anwar Sadiq, Abdul Ullah, Farhat Nawaz, Asif Ullah, Ikram Maaza, Malik |
author_facet | Mohamed, Hamza Elsayed Ahmad Khalil, Ali Talha Hkiri, Khaoula Ayaz, Muhammad Abbasi, Jamil Anwar Sadiq, Abdul Ullah, Farhat Nawaz, Asif Ullah, Ikram Maaza, Malik |
author_sort | Mohamed, Hamza Elsayed Ahmad |
collection | PubMed |
description | Hyphaene thebaica fruits were used for the fabrication of spherical erbium oxide nanoparticles (HT-Er(2)O(3) NP(S)) using a one-step simple bioreduction process. XRD pattern revealed a highly crystalline and pure phase with crystallite size of ~ 7.5 nm, whereas, the W–H plot revealed crystallite size of 11 nm. FTIR spectra revealed characteristic Er-O atomic vibrations in the fingerprint region. Bandgap was obtained as 5.25 eV using K-M function. The physicochemical and morphological nature was established using Raman spectroscopy, reflectance spectroscopy, SAED and HR-TEM. HT-Er(2)O(3) NP(S) were further evaluated for antidiabetic potential in mice using in-vivo and in-vitro bioassays. The synthesized HT-Er(2)O(3) NP(S) were screened for in vitro anti-diabetic potentials against α-glucosidase enzyme and α-amylase enzyme and their antioxidant potential was evaluated using DPPH free radical assay. A dose dependent inhibition was obtained against α-glucosidase (IC(50) 12 μg/mL) and α-amylase (IC(50) 78 μg/mL) while good DPPH free radical scavenging potential (IC(50) 78 μg mL(−1)) is reported. At 1000 μg/mL, the HT-Er(2)O(3) NP(S) revealed 90.30% and 92.30% inhibition of α-amylase and α-glucosidase enzymes. HT-Er(2)O(3) NPs treated groups were observed to have better glycemic control in diabetic animals (503.66 ± 5.92*** on day 0 and 185.66 ± 2.60*** on day 21) when compared with positive control glibenclamide treated group. Further, HT-Er(2)O(3) NP(S) therapy for 21 days caused a considerable effect on serum total lipids, cholesterol, triglycerides, HDL and LDL as compared to untreated diabetic group. In conclusion, our preliminary findings on HT-Er(2)O(3) NP(S) revealed considerable antidiabetic potential and thus can be an effective candidate for controlling the post-prandial hyperglycemia. However, further studies are encouraged especially taking into consideration the toxicity aspects of the nanomaterial. |
format | Online Article Text |
id | pubmed-9968365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-99683652023-02-27 Physicochemical and nanomedicine applications of phyto-reduced erbium oxide (Er(2)O(3)) nanoparticles Mohamed, Hamza Elsayed Ahmad Khalil, Ali Talha Hkiri, Khaoula Ayaz, Muhammad Abbasi, Jamil Anwar Sadiq, Abdul Ullah, Farhat Nawaz, Asif Ullah, Ikram Maaza, Malik AMB Express Original Article Hyphaene thebaica fruits were used for the fabrication of spherical erbium oxide nanoparticles (HT-Er(2)O(3) NP(S)) using a one-step simple bioreduction process. XRD pattern revealed a highly crystalline and pure phase with crystallite size of ~ 7.5 nm, whereas, the W–H plot revealed crystallite size of 11 nm. FTIR spectra revealed characteristic Er-O atomic vibrations in the fingerprint region. Bandgap was obtained as 5.25 eV using K-M function. The physicochemical and morphological nature was established using Raman spectroscopy, reflectance spectroscopy, SAED and HR-TEM. HT-Er(2)O(3) NP(S) were further evaluated for antidiabetic potential in mice using in-vivo and in-vitro bioassays. The synthesized HT-Er(2)O(3) NP(S) were screened for in vitro anti-diabetic potentials against α-glucosidase enzyme and α-amylase enzyme and their antioxidant potential was evaluated using DPPH free radical assay. A dose dependent inhibition was obtained against α-glucosidase (IC(50) 12 μg/mL) and α-amylase (IC(50) 78 μg/mL) while good DPPH free radical scavenging potential (IC(50) 78 μg mL(−1)) is reported. At 1000 μg/mL, the HT-Er(2)O(3) NP(S) revealed 90.30% and 92.30% inhibition of α-amylase and α-glucosidase enzymes. HT-Er(2)O(3) NPs treated groups were observed to have better glycemic control in diabetic animals (503.66 ± 5.92*** on day 0 and 185.66 ± 2.60*** on day 21) when compared with positive control glibenclamide treated group. Further, HT-Er(2)O(3) NP(S) therapy for 21 days caused a considerable effect on serum total lipids, cholesterol, triglycerides, HDL and LDL as compared to untreated diabetic group. In conclusion, our preliminary findings on HT-Er(2)O(3) NP(S) revealed considerable antidiabetic potential and thus can be an effective candidate for controlling the post-prandial hyperglycemia. However, further studies are encouraged especially taking into consideration the toxicity aspects of the nanomaterial. Springer Berlin Heidelberg 2023-02-25 /pmc/articles/PMC9968365/ /pubmed/36840788 http://dx.doi.org/10.1186/s13568-023-01527-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Mohamed, Hamza Elsayed Ahmad Khalil, Ali Talha Hkiri, Khaoula Ayaz, Muhammad Abbasi, Jamil Anwar Sadiq, Abdul Ullah, Farhat Nawaz, Asif Ullah, Ikram Maaza, Malik Physicochemical and nanomedicine applications of phyto-reduced erbium oxide (Er(2)O(3)) nanoparticles |
title | Physicochemical and nanomedicine applications of phyto-reduced erbium oxide (Er(2)O(3)) nanoparticles |
title_full | Physicochemical and nanomedicine applications of phyto-reduced erbium oxide (Er(2)O(3)) nanoparticles |
title_fullStr | Physicochemical and nanomedicine applications of phyto-reduced erbium oxide (Er(2)O(3)) nanoparticles |
title_full_unstemmed | Physicochemical and nanomedicine applications of phyto-reduced erbium oxide (Er(2)O(3)) nanoparticles |
title_short | Physicochemical and nanomedicine applications of phyto-reduced erbium oxide (Er(2)O(3)) nanoparticles |
title_sort | physicochemical and nanomedicine applications of phyto-reduced erbium oxide (er(2)o(3)) nanoparticles |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968365/ https://www.ncbi.nlm.nih.gov/pubmed/36840788 http://dx.doi.org/10.1186/s13568-023-01527-w |
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