SARS-CoV-2 immune complex triggers human monocyte necroptosis

We analyzed the ability of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) itself and SARS-CoV-2-IgG immune complexes to trigger human monocyte necroptosis. SARS-CoV-2 was able to induce monocyte necroptosis dependently of MLKL activation. Necroptosis-associated proteins (RIPK1, RIPK3 a...

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Autores principales: Santos, Leonardo Duarte, Antunes, Krist Helen, Cassão, Gisele, Gonçalves, João Ismael, Abbadi, Bruno Lopes, Bizarro, Cristiano Valim, Basso, Luiz Augusto, Machado, Pablo, de Souza, Ana Paula D., Porto, Bárbara Nery
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968621/
https://www.ncbi.nlm.nih.gov/pubmed/36870284
http://dx.doi.org/10.1016/j.intimp.2023.109954
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author Santos, Leonardo Duarte
Antunes, Krist Helen
Cassão, Gisele
Gonçalves, João Ismael
Abbadi, Bruno Lopes
Bizarro, Cristiano Valim
Basso, Luiz Augusto
Machado, Pablo
de Souza, Ana Paula D.
Porto, Bárbara Nery
author_facet Santos, Leonardo Duarte
Antunes, Krist Helen
Cassão, Gisele
Gonçalves, João Ismael
Abbadi, Bruno Lopes
Bizarro, Cristiano Valim
Basso, Luiz Augusto
Machado, Pablo
de Souza, Ana Paula D.
Porto, Bárbara Nery
author_sort Santos, Leonardo Duarte
collection PubMed
description We analyzed the ability of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) itself and SARS-CoV-2-IgG immune complexes to trigger human monocyte necroptosis. SARS-CoV-2 was able to induce monocyte necroptosis dependently of MLKL activation. Necroptosis-associated proteins (RIPK1, RIPK3 and MLKL) were involved in SARS-CoV-2N1 gene expression in monocytes. SARS-CoV-2 immune complexes promoted monocyte necroptosis in a RIPK3- and MLKL-dependent manner, and Syk tyrosine kinase was necessary for SARS-CoV-2 immune complex-induced monocyte necroptosis, indicating the involvement of Fcγ receptors on necroptosis. Finally, we provide evidence that elevated LDH levels as a marker of lytic cell death are associated with COVID-19 pathogenesis.
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spelling pubmed-99686212023-02-27 SARS-CoV-2 immune complex triggers human monocyte necroptosis Santos, Leonardo Duarte Antunes, Krist Helen Cassão, Gisele Gonçalves, João Ismael Abbadi, Bruno Lopes Bizarro, Cristiano Valim Basso, Luiz Augusto Machado, Pablo de Souza, Ana Paula D. Porto, Bárbara Nery Int Immunopharmacol Article We analyzed the ability of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) itself and SARS-CoV-2-IgG immune complexes to trigger human monocyte necroptosis. SARS-CoV-2 was able to induce monocyte necroptosis dependently of MLKL activation. Necroptosis-associated proteins (RIPK1, RIPK3 and MLKL) were involved in SARS-CoV-2N1 gene expression in monocytes. SARS-CoV-2 immune complexes promoted monocyte necroptosis in a RIPK3- and MLKL-dependent manner, and Syk tyrosine kinase was necessary for SARS-CoV-2 immune complex-induced monocyte necroptosis, indicating the involvement of Fcγ receptors on necroptosis. Finally, we provide evidence that elevated LDH levels as a marker of lytic cell death are associated with COVID-19 pathogenesis. Elsevier B.V. 2023-04 2023-02-27 /pmc/articles/PMC9968621/ /pubmed/36870284 http://dx.doi.org/10.1016/j.intimp.2023.109954 Text en © 2023 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Santos, Leonardo Duarte
Antunes, Krist Helen
Cassão, Gisele
Gonçalves, João Ismael
Abbadi, Bruno Lopes
Bizarro, Cristiano Valim
Basso, Luiz Augusto
Machado, Pablo
de Souza, Ana Paula D.
Porto, Bárbara Nery
SARS-CoV-2 immune complex triggers human monocyte necroptosis
title SARS-CoV-2 immune complex triggers human monocyte necroptosis
title_full SARS-CoV-2 immune complex triggers human monocyte necroptosis
title_fullStr SARS-CoV-2 immune complex triggers human monocyte necroptosis
title_full_unstemmed SARS-CoV-2 immune complex triggers human monocyte necroptosis
title_short SARS-CoV-2 immune complex triggers human monocyte necroptosis
title_sort sars-cov-2 immune complex triggers human monocyte necroptosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9968621/
https://www.ncbi.nlm.nih.gov/pubmed/36870284
http://dx.doi.org/10.1016/j.intimp.2023.109954
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