Cargando…

Early-life dietary exposures mediate persistent shifts in the gut microbiome and visceral fat metabolism

In utero dietary exposures are linked to the development of metabolic syndrome in adult offspring. These dietary exposures can potentially impact gut microbial composition and offspring metabolic health. Female BALB/c mice were administered a lard, lard + flaxseed oil, high sugar, or control diet 4...

Descripción completa

Detalles Bibliográficos
Autores principales: Newman, Tiffany M., Clear, Kenysha Y. J., Wilson, Adam S., Soto-Pantoja, David R., Ochs-Balcom, Heather M., Cook, Katherine L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Physiological Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9970661/
https://www.ncbi.nlm.nih.gov/pubmed/35848617
http://dx.doi.org/10.1152/ajpcell.00380.2021
_version_ 1784897951941787648
author Newman, Tiffany M.
Clear, Kenysha Y. J.
Wilson, Adam S.
Soto-Pantoja, David R.
Ochs-Balcom, Heather M.
Cook, Katherine L.
author_facet Newman, Tiffany M.
Clear, Kenysha Y. J.
Wilson, Adam S.
Soto-Pantoja, David R.
Ochs-Balcom, Heather M.
Cook, Katherine L.
author_sort Newman, Tiffany M.
collection PubMed
description In utero dietary exposures are linked to the development of metabolic syndrome in adult offspring. These dietary exposures can potentially impact gut microbial composition and offspring metabolic health. Female BALB/c mice were administered a lard, lard + flaxseed oil, high sugar, or control diet 4 wk before mating, throughout mating, pregnancy, and lactation. Female offspring were offered low-fat control diet at weaning. Fecal 16S sequencing was performed. Untargeted metabolomics was performed on visceral adipose tissue (VAT) of adult female offspring. Immunohistochemistry was used to determine adipocyte size, VAT collagen deposition, and macrophage content. Hippurate was administered via weekly intraperitoneal injections to low-fat and high-fat diet-fed female mice and VAT fibrosis and collagen 1A (COL1A) were assessed by immunohistochemistry. Lard diet exposure was associated with elevated body and VAT weight and dysregulated glucose metabolism. Lard + flaxseed oil attenuated these effects. Lard diet exposures were associated with increased adipocyte diameter and VAT macrophage count. Lard + flaxseed oil reduced adipocyte diameter and fibrosis compared with the lard diet. Hippurate-associated bacteria were influenced by lard versus lard + flax exposures that persisted to adulthood. VAT hippurate was increased in lard + flaxseed oil compared with lard diet. Hippurate supplementation mitigated VAT fibrosis pathology. Maternal high-fat lard diet consumption resulted in long-term metabolic and gut microbiome programming in offspring, impacting VAT inflammation and fibrosis, and was associated with reduced VAT hippurate content. These traits were not observed in maternal high-fat lard + flaxseed oil diet-exposed offspring. Hippurate supplementation reduced VAT fibrosis. These data suggest that detrimental effects of early-life high-fat lard diet exposure can be attenuated by dietary omega-3 polyunsaturated fatty acid supplementation.
format Online
Article
Text
id pubmed-9970661
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher American Physiological Society
record_format MEDLINE/PubMed
spelling pubmed-99706612023-02-28 Early-life dietary exposures mediate persistent shifts in the gut microbiome and visceral fat metabolism Newman, Tiffany M. Clear, Kenysha Y. J. Wilson, Adam S. Soto-Pantoja, David R. Ochs-Balcom, Heather M. Cook, Katherine L. Am J Physiol Cell Physiol Research Article In utero dietary exposures are linked to the development of metabolic syndrome in adult offspring. These dietary exposures can potentially impact gut microbial composition and offspring metabolic health. Female BALB/c mice were administered a lard, lard + flaxseed oil, high sugar, or control diet 4 wk before mating, throughout mating, pregnancy, and lactation. Female offspring were offered low-fat control diet at weaning. Fecal 16S sequencing was performed. Untargeted metabolomics was performed on visceral adipose tissue (VAT) of adult female offspring. Immunohistochemistry was used to determine adipocyte size, VAT collagen deposition, and macrophage content. Hippurate was administered via weekly intraperitoneal injections to low-fat and high-fat diet-fed female mice and VAT fibrosis and collagen 1A (COL1A) were assessed by immunohistochemistry. Lard diet exposure was associated with elevated body and VAT weight and dysregulated glucose metabolism. Lard + flaxseed oil attenuated these effects. Lard diet exposures were associated with increased adipocyte diameter and VAT macrophage count. Lard + flaxseed oil reduced adipocyte diameter and fibrosis compared with the lard diet. Hippurate-associated bacteria were influenced by lard versus lard + flax exposures that persisted to adulthood. VAT hippurate was increased in lard + flaxseed oil compared with lard diet. Hippurate supplementation mitigated VAT fibrosis pathology. Maternal high-fat lard diet consumption resulted in long-term metabolic and gut microbiome programming in offspring, impacting VAT inflammation and fibrosis, and was associated with reduced VAT hippurate content. These traits were not observed in maternal high-fat lard + flaxseed oil diet-exposed offspring. Hippurate supplementation reduced VAT fibrosis. These data suggest that detrimental effects of early-life high-fat lard diet exposure can be attenuated by dietary omega-3 polyunsaturated fatty acid supplementation. American Physiological Society 2023-03-01 2022-07-18 /pmc/articles/PMC9970661/ /pubmed/35848617 http://dx.doi.org/10.1152/ajpcell.00380.2021 Text en Copyright © 2023 The Authors https://creativecommons.org/licenses/by/4.0/Licensed under Creative Commons Attribution CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/) . Published by the American Physiological Society.
spellingShingle Research Article
Newman, Tiffany M.
Clear, Kenysha Y. J.
Wilson, Adam S.
Soto-Pantoja, David R.
Ochs-Balcom, Heather M.
Cook, Katherine L.
Early-life dietary exposures mediate persistent shifts in the gut microbiome and visceral fat metabolism
title Early-life dietary exposures mediate persistent shifts in the gut microbiome and visceral fat metabolism
title_full Early-life dietary exposures mediate persistent shifts in the gut microbiome and visceral fat metabolism
title_fullStr Early-life dietary exposures mediate persistent shifts in the gut microbiome and visceral fat metabolism
title_full_unstemmed Early-life dietary exposures mediate persistent shifts in the gut microbiome and visceral fat metabolism
title_short Early-life dietary exposures mediate persistent shifts in the gut microbiome and visceral fat metabolism
title_sort early-life dietary exposures mediate persistent shifts in the gut microbiome and visceral fat metabolism
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9970661/
https://www.ncbi.nlm.nih.gov/pubmed/35848617
http://dx.doi.org/10.1152/ajpcell.00380.2021
work_keys_str_mv AT newmantiffanym earlylifedietaryexposuresmediatepersistentshiftsinthegutmicrobiomeandvisceralfatmetabolism
AT clearkenyshayj earlylifedietaryexposuresmediatepersistentshiftsinthegutmicrobiomeandvisceralfatmetabolism
AT wilsonadams earlylifedietaryexposuresmediatepersistentshiftsinthegutmicrobiomeandvisceralfatmetabolism
AT sotopantojadavidr earlylifedietaryexposuresmediatepersistentshiftsinthegutmicrobiomeandvisceralfatmetabolism
AT ochsbalcomheatherm earlylifedietaryexposuresmediatepersistentshiftsinthegutmicrobiomeandvisceralfatmetabolism
AT cookkatherinel earlylifedietaryexposuresmediatepersistentshiftsinthegutmicrobiomeandvisceralfatmetabolism