Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR

Trichophyton verrucosum is the most commonly dermatophyte involved in cattle ringworm. This work reported a case of bovine dermatophytosis due to Trichophyton verrucosum detected from the clinical sample by SYBR-Green real-time PCR. The strategy was based on the DNA extraction directly from the infe...

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Detalles Bibliográficos
Autores principales: Spanamberg, Andréia, Ravazzolo, Ana Paula, Araujo, Ricardo, Franceschi, Natália, Ferreiro, Laerte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9971031/
https://www.ncbi.nlm.nih.gov/pubmed/36865767
http://dx.doi.org/10.1016/j.mmcr.2023.01.002
Descripción
Sumario:Trichophyton verrucosum is the most commonly dermatophyte involved in cattle ringworm. This work reported a case of bovine dermatophytosis due to Trichophyton verrucosum detected from the clinical sample by SYBR-Green real-time PCR. The strategy was based on the DNA extraction directly from the infected hair followed by real‐time PCR and melting‐point analysis. A faster and differential diagnosis was observed when compared to the conventional mycological methodology for detection and identification of Trichophyton verrucosum.

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