Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR

Trichophyton verrucosum is the most commonly dermatophyte involved in cattle ringworm. This work reported a case of bovine dermatophytosis due to Trichophyton verrucosum detected from the clinical sample by SYBR-Green real-time PCR. The strategy was based on the DNA extraction directly from the infe...

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Autores principales: Spanamberg, Andréia, Ravazzolo, Ana Paula, Araujo, Ricardo, Franceschi, Natália, Ferreiro, Laerte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9971031/
https://www.ncbi.nlm.nih.gov/pubmed/36865767
http://dx.doi.org/10.1016/j.mmcr.2023.01.002
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author Spanamberg, Andréia
Ravazzolo, Ana Paula
Araujo, Ricardo
Franceschi, Natália
Ferreiro, Laerte
author_facet Spanamberg, Andréia
Ravazzolo, Ana Paula
Araujo, Ricardo
Franceschi, Natália
Ferreiro, Laerte
author_sort Spanamberg, Andréia
collection PubMed
description Trichophyton verrucosum is the most commonly dermatophyte involved in cattle ringworm. This work reported a case of bovine dermatophytosis due to Trichophyton verrucosum detected from the clinical sample by SYBR-Green real-time PCR. The strategy was based on the DNA extraction directly from the infected hair followed by real‐time PCR and melting‐point analysis. A faster and differential diagnosis was observed when compared to the conventional mycological methodology for detection and identification of Trichophyton verrucosum.
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spelling pubmed-99710312023-03-01 Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR Spanamberg, Andréia Ravazzolo, Ana Paula Araujo, Ricardo Franceschi, Natália Ferreiro, Laerte Med Mycol Case Rep Case Report Trichophyton verrucosum is the most commonly dermatophyte involved in cattle ringworm. This work reported a case of bovine dermatophytosis due to Trichophyton verrucosum detected from the clinical sample by SYBR-Green real-time PCR. The strategy was based on the DNA extraction directly from the infected hair followed by real‐time PCR and melting‐point analysis. A faster and differential diagnosis was observed when compared to the conventional mycological methodology for detection and identification of Trichophyton verrucosum. Elsevier 2023-01-31 /pmc/articles/PMC9971031/ /pubmed/36865767 http://dx.doi.org/10.1016/j.mmcr.2023.01.002 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Case Report
Spanamberg, Andréia
Ravazzolo, Ana Paula
Araujo, Ricardo
Franceschi, Natália
Ferreiro, Laerte
Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR
title Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR
title_full Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR
title_fullStr Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR
title_full_unstemmed Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR
title_short Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR
title_sort bovine ringworm - detection of trichophyton verrucosum by sybr-green real-time pcr
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9971031/
https://www.ncbi.nlm.nih.gov/pubmed/36865767
http://dx.doi.org/10.1016/j.mmcr.2023.01.002
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