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Advanced glycation end products regulate the receptor of AGEs epigenetically
Advanced glycation end-products (AGEs) can boost their receptor of AGE (RAGE) expression through the downstream signaling pathway to facilitate AGE–RAGE interaction. In this regulation process, the primary signaling pathways are NF-κB and STAT3. However, the inhibition of these transcription factors...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9971228/ https://www.ncbi.nlm.nih.gov/pubmed/36866277 http://dx.doi.org/10.3389/fcell.2023.1062229 |
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author | Wu, Xiaoqing Shi, Xuanren Chen, Xiaoyong Yin, Zhanhai |
author_facet | Wu, Xiaoqing Shi, Xuanren Chen, Xiaoyong Yin, Zhanhai |
author_sort | Wu, Xiaoqing |
collection | PubMed |
description | Advanced glycation end-products (AGEs) can boost their receptor of AGE (RAGE) expression through the downstream signaling pathway to facilitate AGE–RAGE interaction. In this regulation process, the primary signaling pathways are NF-κB and STAT3. However, the inhibition of these transcription factors cannot completely block the upregulation of RAGE, which indicates AGEs may also impact RAGE expression via other pathways. In this study, we revealed that AGEs can exhibit epigenetic impacts on RAGE expression. Here, we used carboxymethyl-lysine (CML) and carboxyethyl-lysine (CEL) to treat liver cells and discovered that AGEs can promote the demethylation of the RAGE promoter region. To verify this epigenetic modification, we employed dCAS9-DNMT3a with sgRNA to specifically modify the RAGE promoter region against the effect of carboxymethyl-lysine and carboxyethyl-lysine. The elevated RAGE expressions were partially repressed after AGE-induced hypomethylation statuses were reversed. Additionally, TET1 were also upregulated in AGE-treated cells, indicating AGEs may epigenetically modulate RAGE through the elevating TET1 level. |
format | Online Article Text |
id | pubmed-9971228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99712282023-03-01 Advanced glycation end products regulate the receptor of AGEs epigenetically Wu, Xiaoqing Shi, Xuanren Chen, Xiaoyong Yin, Zhanhai Front Cell Dev Biol Cell and Developmental Biology Advanced glycation end-products (AGEs) can boost their receptor of AGE (RAGE) expression through the downstream signaling pathway to facilitate AGE–RAGE interaction. In this regulation process, the primary signaling pathways are NF-κB and STAT3. However, the inhibition of these transcription factors cannot completely block the upregulation of RAGE, which indicates AGEs may also impact RAGE expression via other pathways. In this study, we revealed that AGEs can exhibit epigenetic impacts on RAGE expression. Here, we used carboxymethyl-lysine (CML) and carboxyethyl-lysine (CEL) to treat liver cells and discovered that AGEs can promote the demethylation of the RAGE promoter region. To verify this epigenetic modification, we employed dCAS9-DNMT3a with sgRNA to specifically modify the RAGE promoter region against the effect of carboxymethyl-lysine and carboxyethyl-lysine. The elevated RAGE expressions were partially repressed after AGE-induced hypomethylation statuses were reversed. Additionally, TET1 were also upregulated in AGE-treated cells, indicating AGEs may epigenetically modulate RAGE through the elevating TET1 level. Frontiers Media S.A. 2023-02-14 /pmc/articles/PMC9971228/ /pubmed/36866277 http://dx.doi.org/10.3389/fcell.2023.1062229 Text en Copyright © 2023 Wu, Shi, Chen and Yin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Wu, Xiaoqing Shi, Xuanren Chen, Xiaoyong Yin, Zhanhai Advanced glycation end products regulate the receptor of AGEs epigenetically |
title | Advanced glycation end products regulate the receptor of AGEs epigenetically |
title_full | Advanced glycation end products regulate the receptor of AGEs epigenetically |
title_fullStr | Advanced glycation end products regulate the receptor of AGEs epigenetically |
title_full_unstemmed | Advanced glycation end products regulate the receptor of AGEs epigenetically |
title_short | Advanced glycation end products regulate the receptor of AGEs epigenetically |
title_sort | advanced glycation end products regulate the receptor of ages epigenetically |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9971228/ https://www.ncbi.nlm.nih.gov/pubmed/36866277 http://dx.doi.org/10.3389/fcell.2023.1062229 |
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