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Implementing molecular tuberculosis diagnostic methods in limited-resource and high-burden countries
Tuberculosis (TB) is one of the deadliest infectious diseases in the world with more than a million people dying of TB each year. Accurate and timely TB diagnosis has the potential to alleviate the global TB burden; therefore, one of the pillars of the End TB Strategy developed by the World Health O...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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European Respiratory Society
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9973455/ https://www.ncbi.nlm.nih.gov/pubmed/36865933 http://dx.doi.org/10.1183/20734735.0226-2022 |
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author | Vasiliu, Anca Saktiawati, Antonia Morita Iswari Duarte, Raquel Lange, Christoph Cirillo, Daniela Maria |
author_facet | Vasiliu, Anca Saktiawati, Antonia Morita Iswari Duarte, Raquel Lange, Christoph Cirillo, Daniela Maria |
author_sort | Vasiliu, Anca |
collection | PubMed |
description | Tuberculosis (TB) is one of the deadliest infectious diseases in the world with more than a million people dying of TB each year. Accurate and timely TB diagnosis has the potential to alleviate the global TB burden; therefore, one of the pillars of the End TB Strategy developed by the World Health Organization (WHO) is the early diagnosis of TB, including universal drug-susceptibility testing (DST). The WHO emphasises the importance of DST before treatment initiation, using molecular WHO-recommended rapid diagnostic tests (mWRDs). Currently available mWRDs are nucleic acid amplification tests, line probe assays, whole genome sequencing, and targeted next-generation sequencing. However, implementing the sequencing mWRDs in routine laboratories in low-income countries is constrained by the existing infrastructure, high cost, the specialised skills needed, data storage, and the current delay in results compared with other routine methods. These limitations are pronounced in resource-limited settings, which often have a high TB burden and need for innovative TB diagnostic technologies. In this article we propose several possible solutions, like adapting infrastructure capacity to needs, advocating for lowering costs, building bioinformatics and laboratory capacity, and increasing the use of open-access resources for software and publications. |
format | Online Article Text |
id | pubmed-9973455 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | European Respiratory Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-99734552023-03-01 Implementing molecular tuberculosis diagnostic methods in limited-resource and high-burden countries Vasiliu, Anca Saktiawati, Antonia Morita Iswari Duarte, Raquel Lange, Christoph Cirillo, Daniela Maria Breathe (Sheff) Viewpoint Tuberculosis (TB) is one of the deadliest infectious diseases in the world with more than a million people dying of TB each year. Accurate and timely TB diagnosis has the potential to alleviate the global TB burden; therefore, one of the pillars of the End TB Strategy developed by the World Health Organization (WHO) is the early diagnosis of TB, including universal drug-susceptibility testing (DST). The WHO emphasises the importance of DST before treatment initiation, using molecular WHO-recommended rapid diagnostic tests (mWRDs). Currently available mWRDs are nucleic acid amplification tests, line probe assays, whole genome sequencing, and targeted next-generation sequencing. However, implementing the sequencing mWRDs in routine laboratories in low-income countries is constrained by the existing infrastructure, high cost, the specialised skills needed, data storage, and the current delay in results compared with other routine methods. These limitations are pronounced in resource-limited settings, which often have a high TB burden and need for innovative TB diagnostic technologies. In this article we propose several possible solutions, like adapting infrastructure capacity to needs, advocating for lowering costs, building bioinformatics and laboratory capacity, and increasing the use of open-access resources for software and publications. European Respiratory Society 2022-12 2023-02-14 /pmc/articles/PMC9973455/ /pubmed/36865933 http://dx.doi.org/10.1183/20734735.0226-2022 Text en Copyright ©ERS 2023 https://creativecommons.org/licenses/by-nc/4.0/Breathe articles are open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. |
spellingShingle | Viewpoint Vasiliu, Anca Saktiawati, Antonia Morita Iswari Duarte, Raquel Lange, Christoph Cirillo, Daniela Maria Implementing molecular tuberculosis diagnostic methods in limited-resource and high-burden countries |
title | Implementing molecular tuberculosis diagnostic methods in limited-resource and high-burden countries |
title_full | Implementing molecular tuberculosis diagnostic methods in limited-resource and high-burden countries |
title_fullStr | Implementing molecular tuberculosis diagnostic methods in limited-resource and high-burden countries |
title_full_unstemmed | Implementing molecular tuberculosis diagnostic methods in limited-resource and high-burden countries |
title_short | Implementing molecular tuberculosis diagnostic methods in limited-resource and high-burden countries |
title_sort | implementing molecular tuberculosis diagnostic methods in limited-resource and high-burden countries |
topic | Viewpoint |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9973455/ https://www.ncbi.nlm.nih.gov/pubmed/36865933 http://dx.doi.org/10.1183/20734735.0226-2022 |
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