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Comparative analysis of magnetically activated cell sorting and ultracentrifugation methods for exosome isolation

Mesenchymal stem cell-derived exosomes regulate cell migration, proliferation, differentiation, and synthesis of the extracellular matrix, giving great potential for the treatment of different diseases. The ultracentrifugation method is the gold standard method for exosome isolation due to the simpl...

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Autores principales: Ciftci, Eda, Bozbeyoglu, Naz, Gursel, Ihsan, Korkusuz, Feza, Bakan Misirlioglu, Feray, Korkusuz, Petek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9974127/
https://www.ncbi.nlm.nih.gov/pubmed/36854030
http://dx.doi.org/10.1371/journal.pone.0282238
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author Ciftci, Eda
Bozbeyoglu, Naz
Gursel, Ihsan
Korkusuz, Feza
Bakan Misirlioglu, Feray
Korkusuz, Petek
author_facet Ciftci, Eda
Bozbeyoglu, Naz
Gursel, Ihsan
Korkusuz, Feza
Bakan Misirlioglu, Feray
Korkusuz, Petek
author_sort Ciftci, Eda
collection PubMed
description Mesenchymal stem cell-derived exosomes regulate cell migration, proliferation, differentiation, and synthesis of the extracellular matrix, giving great potential for the treatment of different diseases. The ultracentrifugation method is the gold standard method for exosome isolation due to the simple protocol, and high yield, but presents low purity and requires specialized equipment. Amelioration of technical optimization is required for quick and reliable confinement of exosomes to translate them to the clinic as cell therapeutics In this study, we hypothesized that magnetically activated cell sorting may provide, an effective, reliable, and rapid tool for exosome isolation when compared to ultracentrifugation. We, therefore, aimed to compare the efficiency of magnetically activated cell sorting and ultracentrifugation for human mesenchymal stem cell-derived exosome isolation from culture media by protein quantification, surface biomarker, size, number, and morphological analysis. Magnetically activated cell sorting provided a higher purity and amount of exosomes that carry visible magnetic beads when compared to ultracentrifugation. The particle number of the magnetically activated cell sorting group was higher than the ultracentrifugation. In conclusion, magnetically activated cell sorting presents a quick, and reliable method to collect and present human mesenchymal stem cell exosomes to clinics at high purity for potential cellular therapeutic approaches. The novel isolation and purification method may be extended to different clinical protocols using different autogenic or allogeneic cell sources.
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spelling pubmed-99741272023-03-01 Comparative analysis of magnetically activated cell sorting and ultracentrifugation methods for exosome isolation Ciftci, Eda Bozbeyoglu, Naz Gursel, Ihsan Korkusuz, Feza Bakan Misirlioglu, Feray Korkusuz, Petek PLoS One Research Article Mesenchymal stem cell-derived exosomes regulate cell migration, proliferation, differentiation, and synthesis of the extracellular matrix, giving great potential for the treatment of different diseases. The ultracentrifugation method is the gold standard method for exosome isolation due to the simple protocol, and high yield, but presents low purity and requires specialized equipment. Amelioration of technical optimization is required for quick and reliable confinement of exosomes to translate them to the clinic as cell therapeutics In this study, we hypothesized that magnetically activated cell sorting may provide, an effective, reliable, and rapid tool for exosome isolation when compared to ultracentrifugation. We, therefore, aimed to compare the efficiency of magnetically activated cell sorting and ultracentrifugation for human mesenchymal stem cell-derived exosome isolation from culture media by protein quantification, surface biomarker, size, number, and morphological analysis. Magnetically activated cell sorting provided a higher purity and amount of exosomes that carry visible magnetic beads when compared to ultracentrifugation. The particle number of the magnetically activated cell sorting group was higher than the ultracentrifugation. In conclusion, magnetically activated cell sorting presents a quick, and reliable method to collect and present human mesenchymal stem cell exosomes to clinics at high purity for potential cellular therapeutic approaches. The novel isolation and purification method may be extended to different clinical protocols using different autogenic or allogeneic cell sources. Public Library of Science 2023-02-28 /pmc/articles/PMC9974127/ /pubmed/36854030 http://dx.doi.org/10.1371/journal.pone.0282238 Text en © 2023 Ciftci et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ciftci, Eda
Bozbeyoglu, Naz
Gursel, Ihsan
Korkusuz, Feza
Bakan Misirlioglu, Feray
Korkusuz, Petek
Comparative analysis of magnetically activated cell sorting and ultracentrifugation methods for exosome isolation
title Comparative analysis of magnetically activated cell sorting and ultracentrifugation methods for exosome isolation
title_full Comparative analysis of magnetically activated cell sorting and ultracentrifugation methods for exosome isolation
title_fullStr Comparative analysis of magnetically activated cell sorting and ultracentrifugation methods for exosome isolation
title_full_unstemmed Comparative analysis of magnetically activated cell sorting and ultracentrifugation methods for exosome isolation
title_short Comparative analysis of magnetically activated cell sorting and ultracentrifugation methods for exosome isolation
title_sort comparative analysis of magnetically activated cell sorting and ultracentrifugation methods for exosome isolation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9974127/
https://www.ncbi.nlm.nih.gov/pubmed/36854030
http://dx.doi.org/10.1371/journal.pone.0282238
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