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Modeling human liver organ development and diseases with pluripotent stem cell-derived organoids

The discoveries of human pluripotent stem cells (PSCs) including embryonic stem cells and induced pluripotent stem cells (iPSCs) has led to dramatic advances in our understanding of basic human developmental and cell biology and has also been applied to research aimed at drug discovery and developme...

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Autores principales: Ouchi, Rie, Koike, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9974642/
https://www.ncbi.nlm.nih.gov/pubmed/36875751
http://dx.doi.org/10.3389/fcell.2023.1133534
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author Ouchi, Rie
Koike, Hiroyuki
author_facet Ouchi, Rie
Koike, Hiroyuki
author_sort Ouchi, Rie
collection PubMed
description The discoveries of human pluripotent stem cells (PSCs) including embryonic stem cells and induced pluripotent stem cells (iPSCs) has led to dramatic advances in our understanding of basic human developmental and cell biology and has also been applied to research aimed at drug discovery and development of disease treatments. Research using human PSCs has been largely dominated by studies using two-dimensional cultures. In the past decade, however, ex vivo tissue “organoids,” which have a complex and functional three-dimensional structure similar to human organs, have been created from PSCs and are now being used in various fields. Organoids created from PSCs are composed of multiple cell types and are valuable models with which it is better to reproduce the complex structures of living organs and study organogenesis through niche reproduction and pathological modeling through cell-cell interactions. Organoids derived from iPSCs, which inherit the genetic background of the donor, are helpful for disease modeling, elucidation of pathophysiology, and drug screening. Moreover, it is anticipated that iPSC-derived organoids will contribute significantly to regenerative medicine by providing treatment alternatives to organ transplantation with which the risk of immune rejection is low. This review summarizes how PSC-derived organoids are used in developmental biology, disease modeling, drug discovery, and regenerative medicine. Highlighted is the liver, an organ that play crucial roles in metabolic regulation and is composed of diverse cell types.
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spelling pubmed-99746422023-03-02 Modeling human liver organ development and diseases with pluripotent stem cell-derived organoids Ouchi, Rie Koike, Hiroyuki Front Cell Dev Biol Cell and Developmental Biology The discoveries of human pluripotent stem cells (PSCs) including embryonic stem cells and induced pluripotent stem cells (iPSCs) has led to dramatic advances in our understanding of basic human developmental and cell biology and has also been applied to research aimed at drug discovery and development of disease treatments. Research using human PSCs has been largely dominated by studies using two-dimensional cultures. In the past decade, however, ex vivo tissue “organoids,” which have a complex and functional three-dimensional structure similar to human organs, have been created from PSCs and are now being used in various fields. Organoids created from PSCs are composed of multiple cell types and are valuable models with which it is better to reproduce the complex structures of living organs and study organogenesis through niche reproduction and pathological modeling through cell-cell interactions. Organoids derived from iPSCs, which inherit the genetic background of the donor, are helpful for disease modeling, elucidation of pathophysiology, and drug screening. Moreover, it is anticipated that iPSC-derived organoids will contribute significantly to regenerative medicine by providing treatment alternatives to organ transplantation with which the risk of immune rejection is low. This review summarizes how PSC-derived organoids are used in developmental biology, disease modeling, drug discovery, and regenerative medicine. Highlighted is the liver, an organ that play crucial roles in metabolic regulation and is composed of diverse cell types. Frontiers Media S.A. 2023-02-15 /pmc/articles/PMC9974642/ /pubmed/36875751 http://dx.doi.org/10.3389/fcell.2023.1133534 Text en Copyright © 2023 Ouchi and Koike. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Ouchi, Rie
Koike, Hiroyuki
Modeling human liver organ development and diseases with pluripotent stem cell-derived organoids
title Modeling human liver organ development and diseases with pluripotent stem cell-derived organoids
title_full Modeling human liver organ development and diseases with pluripotent stem cell-derived organoids
title_fullStr Modeling human liver organ development and diseases with pluripotent stem cell-derived organoids
title_full_unstemmed Modeling human liver organ development and diseases with pluripotent stem cell-derived organoids
title_short Modeling human liver organ development and diseases with pluripotent stem cell-derived organoids
title_sort modeling human liver organ development and diseases with pluripotent stem cell-derived organoids
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9974642/
https://www.ncbi.nlm.nih.gov/pubmed/36875751
http://dx.doi.org/10.3389/fcell.2023.1133534
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