Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase

We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for c...

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Detalles Bibliográficos
Autores principales: Beneke, Tom, Dobramysl, Ulrich, Catta-Preta, Carolina Moura Costa, Mottram, Jeremy Charles, Gluenz, Eva, Wheeler, Richard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2023
Materias:
Data Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975418/
https://www.ncbi.nlm.nih.gov/pubmed/36874584
http://dx.doi.org/10.12688/wellcomeopenres.18575.2
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author Beneke, Tom
Dobramysl, Ulrich
Catta-Preta, Carolina Moura Costa
Mottram, Jeremy Charles
Gluenz, Eva
Wheeler, Richard J.
author_facet Beneke, Tom
Dobramysl, Ulrich
Catta-Preta, Carolina Moura Costa
Mottram, Jeremy Charles
Gluenz, Eva
Wheeler, Richard J.
author_sort Beneke, Tom
collection PubMed
description We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.
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spelling pubmed-99754182023-03-02 Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase Beneke, Tom Dobramysl, Ulrich Catta-Preta, Carolina Moura Costa Mottram, Jeremy Charles Gluenz, Eva Wheeler, Richard J. Wellcome Open Res Data Note We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging. F1000 Research Limited 2023-02-23 /pmc/articles/PMC9975418/ /pubmed/36874584 http://dx.doi.org/10.12688/wellcomeopenres.18575.2 Text en Copyright: © 2023 Beneke T et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Data Note
Beneke, Tom
Dobramysl, Ulrich
Catta-Preta, Carolina Moura Costa
Mottram, Jeremy Charles
Gluenz, Eva
Wheeler, Richard J.
Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase
title Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase
title_full Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase
title_fullStr Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase
title_full_unstemmed Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase
title_short Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase
title_sort genome sequence of leishmania mexicana mnyc/bz/62/m379 expressing cas9 and t7 rna polymerase
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975418/
https://www.ncbi.nlm.nih.gov/pubmed/36874584
http://dx.doi.org/10.12688/wellcomeopenres.18575.2
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