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GbFLSa overexpression negatively regulates proanthocyanin biosynthesis

Flavonoids are important secondary metabolites with extensive pharmacological functions. Ginkgo biloba L. (ginkgo) has attracted extensive attention because of its high flavonoid medicinal value. However, little is understood about ginkgo flavonol biosynthesis. Herein, we cloned the full-length ging...

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Autores principales: Guo, Jing, Wu, Yaqiong, Wang, Tongli, Xin, Yue, Wang, Guibin, Zhou, Qi, Xu, Li-An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975577/
https://www.ncbi.nlm.nih.gov/pubmed/36875575
http://dx.doi.org/10.3389/fpls.2023.1093656
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author Guo, Jing
Wu, Yaqiong
Wang, Tongli
Xin, Yue
Wang, Guibin
Zhou, Qi
Xu, Li-An
author_facet Guo, Jing
Wu, Yaqiong
Wang, Tongli
Xin, Yue
Wang, Guibin
Zhou, Qi
Xu, Li-An
author_sort Guo, Jing
collection PubMed
description Flavonoids are important secondary metabolites with extensive pharmacological functions. Ginkgo biloba L. (ginkgo) has attracted extensive attention because of its high flavonoid medicinal value. However, little is understood about ginkgo flavonol biosynthesis. Herein, we cloned the full-length gingko GbFLSa gene (1314 bp), which encodes a 363 amino acid protein that has a typical 2-oxoglutarate (2OG)-Fe(II) oxygenase region. Recombinant GbFLSa protein with a molecular mass of 41 kDa was expressed in Escherichia coli BL21(DE3). The protein was localized to the cytoplasm. Moreover, proanthocyanins, including catechin, epicatechin, epigallocatechin and gallocatechin, were significantly less abundant in transgenic poplar than in nontransgenic (CK) plants. In addition, dihydroflavonol 4-reductase, anthocyanidin synthase and leucoanthocyanidin reductase expression levels were significantly lower than those of their CK counterparts. GbFLSa thus encodes a functional protein that might negatively regulate proanthocyanin biosynthesis. This study helps elucidate the role of GbFLSa in plant metabolism and the potential molecular mechanism of flavonoid biosynthesis.
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spelling pubmed-99755772023-03-02 GbFLSa overexpression negatively regulates proanthocyanin biosynthesis Guo, Jing Wu, Yaqiong Wang, Tongli Xin, Yue Wang, Guibin Zhou, Qi Xu, Li-An Front Plant Sci Plant Science Flavonoids are important secondary metabolites with extensive pharmacological functions. Ginkgo biloba L. (ginkgo) has attracted extensive attention because of its high flavonoid medicinal value. However, little is understood about ginkgo flavonol biosynthesis. Herein, we cloned the full-length gingko GbFLSa gene (1314 bp), which encodes a 363 amino acid protein that has a typical 2-oxoglutarate (2OG)-Fe(II) oxygenase region. Recombinant GbFLSa protein with a molecular mass of 41 kDa was expressed in Escherichia coli BL21(DE3). The protein was localized to the cytoplasm. Moreover, proanthocyanins, including catechin, epicatechin, epigallocatechin and gallocatechin, were significantly less abundant in transgenic poplar than in nontransgenic (CK) plants. In addition, dihydroflavonol 4-reductase, anthocyanidin synthase and leucoanthocyanidin reductase expression levels were significantly lower than those of their CK counterparts. GbFLSa thus encodes a functional protein that might negatively regulate proanthocyanin biosynthesis. This study helps elucidate the role of GbFLSa in plant metabolism and the potential molecular mechanism of flavonoid biosynthesis. Frontiers Media S.A. 2023-02-15 /pmc/articles/PMC9975577/ /pubmed/36875575 http://dx.doi.org/10.3389/fpls.2023.1093656 Text en Copyright © 2023 Guo, Wu, Wang, Xin, Wang, Zhou and Xu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Guo, Jing
Wu, Yaqiong
Wang, Tongli
Xin, Yue
Wang, Guibin
Zhou, Qi
Xu, Li-An
GbFLSa overexpression negatively regulates proanthocyanin biosynthesis
title GbFLSa overexpression negatively regulates proanthocyanin biosynthesis
title_full GbFLSa overexpression negatively regulates proanthocyanin biosynthesis
title_fullStr GbFLSa overexpression negatively regulates proanthocyanin biosynthesis
title_full_unstemmed GbFLSa overexpression negatively regulates proanthocyanin biosynthesis
title_short GbFLSa overexpression negatively regulates proanthocyanin biosynthesis
title_sort gbflsa overexpression negatively regulates proanthocyanin biosynthesis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975577/
https://www.ncbi.nlm.nih.gov/pubmed/36875575
http://dx.doi.org/10.3389/fpls.2023.1093656
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