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Lung protective effects of dietary malate esters derivatives from Bletilla striata against SiO(2) nanoparticles through activation of Nrf2 pathway

OBJECTIVE: To study the protective activities of the dietary malate esters derivatives of Bletilla striata against SiO(2) nanoparticles-induced A549 cell lines and its mechanism action. METHODS: The components were isolated and elucidated by spectroscopic methods such as 1D NMR and 2D NMR. And MTT a...

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Detalles Bibliográficos
Autores principales: Zhou, Di, Chang, Wenhui, Qi, Jiaxin, Chen, Gang, Li, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975635/
https://www.ncbi.nlm.nih.gov/pubmed/36875434
http://dx.doi.org/10.1016/j.chmed.2022.11.001
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author Zhou, Di
Chang, Wenhui
Qi, Jiaxin
Chen, Gang
Li, Ning
author_facet Zhou, Di
Chang, Wenhui
Qi, Jiaxin
Chen, Gang
Li, Ning
author_sort Zhou, Di
collection PubMed
description OBJECTIVE: To study the protective activities of the dietary malate esters derivatives of Bletilla striata against SiO(2) nanoparticles-induced A549 cell lines and its mechanism action. METHODS: The components were isolated and elucidated by spectroscopic methods such as 1D NMR and 2D NMR. And MTT assays was used to tested these components on the A549 cell survival rates and ROS or proteins levels were detected by Western blotting. RESULTS: A new glucosyloxybenzyl 2-isobutylmalate (a malate ester derivative), along with 31 known compounds were isolated and identified from n-BuOH extract of EtOH extract of B. striata. Among them, compounds 3, 4, 11, 12 and 13 possessed noteworthy proliferative effects for damaged cells, with ED(50) of 14.0, 13.1, 3.7, 11.6 and 11.5 µmol/L, respectively, compared to positive control resveratrol (ED(50), 14.7 µmol/L). Militarine (8) prominently inhibited the intracellular ROS level, and increased the expression of Nrf2 and its downstream genes (HO-1 and γ-GCSc). Furthermore, Nrf2 activation mediates the interventional effects of compound 8 against SiO(2) nanoparticles (nm SiO(2))-induced lung injury. Moreover, treatment with compound 8 significantly reduced lung inflammation and oxidative stress in nm SiO(2)-instilled mice. Molecular docking experiment suggested that 8 bound stably to the HO-1 protein by hydrogen bond interactions. CONCLUSION: The dietary malate esters derivatives of B. striata could significantly increase the viability of nm SiO(2)-induced A549 cells and decrease the finer particles-induced cell damages. Militarine is especially promising compound for chemoprevention of lung cancer induced by nm SiO(2) through activation of Nrf2 pathway.
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spelling pubmed-99756352023-03-02 Lung protective effects of dietary malate esters derivatives from Bletilla striata against SiO(2) nanoparticles through activation of Nrf2 pathway Zhou, Di Chang, Wenhui Qi, Jiaxin Chen, Gang Li, Ning Chin Herb Med Original Article OBJECTIVE: To study the protective activities of the dietary malate esters derivatives of Bletilla striata against SiO(2) nanoparticles-induced A549 cell lines and its mechanism action. METHODS: The components were isolated and elucidated by spectroscopic methods such as 1D NMR and 2D NMR. And MTT assays was used to tested these components on the A549 cell survival rates and ROS or proteins levels were detected by Western blotting. RESULTS: A new glucosyloxybenzyl 2-isobutylmalate (a malate ester derivative), along with 31 known compounds were isolated and identified from n-BuOH extract of EtOH extract of B. striata. Among them, compounds 3, 4, 11, 12 and 13 possessed noteworthy proliferative effects for damaged cells, with ED(50) of 14.0, 13.1, 3.7, 11.6 and 11.5 µmol/L, respectively, compared to positive control resveratrol (ED(50), 14.7 µmol/L). Militarine (8) prominently inhibited the intracellular ROS level, and increased the expression of Nrf2 and its downstream genes (HO-1 and γ-GCSc). Furthermore, Nrf2 activation mediates the interventional effects of compound 8 against SiO(2) nanoparticles (nm SiO(2))-induced lung injury. Moreover, treatment with compound 8 significantly reduced lung inflammation and oxidative stress in nm SiO(2)-instilled mice. Molecular docking experiment suggested that 8 bound stably to the HO-1 protein by hydrogen bond interactions. CONCLUSION: The dietary malate esters derivatives of B. striata could significantly increase the viability of nm SiO(2)-induced A549 cells and decrease the finer particles-induced cell damages. Militarine is especially promising compound for chemoprevention of lung cancer induced by nm SiO(2) through activation of Nrf2 pathway. Elsevier 2022-11-04 /pmc/articles/PMC9975635/ /pubmed/36875434 http://dx.doi.org/10.1016/j.chmed.2022.11.001 Text en © 2022 Tianjin Press of Chinese Herbal Medicines. Published by ELSEVIER B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Zhou, Di
Chang, Wenhui
Qi, Jiaxin
Chen, Gang
Li, Ning
Lung protective effects of dietary malate esters derivatives from Bletilla striata against SiO(2) nanoparticles through activation of Nrf2 pathway
title Lung protective effects of dietary malate esters derivatives from Bletilla striata against SiO(2) nanoparticles through activation of Nrf2 pathway
title_full Lung protective effects of dietary malate esters derivatives from Bletilla striata against SiO(2) nanoparticles through activation of Nrf2 pathway
title_fullStr Lung protective effects of dietary malate esters derivatives from Bletilla striata against SiO(2) nanoparticles through activation of Nrf2 pathway
title_full_unstemmed Lung protective effects of dietary malate esters derivatives from Bletilla striata against SiO(2) nanoparticles through activation of Nrf2 pathway
title_short Lung protective effects of dietary malate esters derivatives from Bletilla striata against SiO(2) nanoparticles through activation of Nrf2 pathway
title_sort lung protective effects of dietary malate esters derivatives from bletilla striata against sio(2) nanoparticles through activation of nrf2 pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975635/
https://www.ncbi.nlm.nih.gov/pubmed/36875434
http://dx.doi.org/10.1016/j.chmed.2022.11.001
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