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Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria)

The fruit peel color is an important factor that affects its quality. However, genes involved in regulating pericarp color in bottle gourd (Lagenaria siceraria) have not been explored to date. Genetic analysis of color traits in bottle gourd peel through a genetic population of six generations demon...

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Autores principales: Huo, Yulai, Zhang, Gui, Yu, Wenjin, Liu, Zhengguo, Shen, Mujie, Zhao, Rongchong, Hu, Shengping, Zheng, Xuyang, Wang, Peng, Yang, Yanjuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975725/
https://www.ncbi.nlm.nih.gov/pubmed/36875578
http://dx.doi.org/10.3389/fpls.2023.1130669
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author Huo, Yulai
Zhang, Gui
Yu, Wenjin
Liu, Zhengguo
Shen, Mujie
Zhao, Rongchong
Hu, Shengping
Zheng, Xuyang
Wang, Peng
Yang, Yanjuan
author_facet Huo, Yulai
Zhang, Gui
Yu, Wenjin
Liu, Zhengguo
Shen, Mujie
Zhao, Rongchong
Hu, Shengping
Zheng, Xuyang
Wang, Peng
Yang, Yanjuan
author_sort Huo, Yulai
collection PubMed
description The fruit peel color is an important factor that affects its quality. However, genes involved in regulating pericarp color in bottle gourd (Lagenaria siceraria) have not been explored to date. Genetic analysis of color traits in bottle gourd peel through a genetic population of six generations demonstrated that the green color of peels is inherited as a single gene dominant trait. Combined phenotype-genotype analysis of recombinant plants using BSA-seq mapped the candidate gene to a 22.645 Kb interval at the head end of chromosome 1. We observed that the final interval contained only one gene, LsAPRR2 (HG_GLEAN_10010973). Sequence and spatiotemporal expression analyses of LsAPRR2 unraveled two nonsynonymous mutations (A→G) and (G→C) in the parental CDS sequences. Further, LsAPRR2 expression was higher in all green-skinned bottle gourds (H16) at various stages of fruit development than in white-skinned bottle gourds (H06). Cloning and sequence comparison of the two parental LsAPRR2 promoter regions indicated 11 bases insertion and 8 SNPs mutations in the region -991~-1033, upstream of the start codon in white bottle gourd. Proof of GUS reporting system, Genetic variation in this fragment significantly reduced the expression of LsAPRR2 in the pericarp of white bottle gourd. In addition, we developed a tightly linked (accuracy 93.88%) InDel marker for the promoter variant segment. Overall, the current study provides a theoretical basis for comprehensive elucidation of the regulatory mechanisms underlying the determination of bottle gourd pericarp color. This would further help in the directed molecular design breeding of bottle gourd pericarp.
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spelling pubmed-99757252023-03-02 Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria) Huo, Yulai Zhang, Gui Yu, Wenjin Liu, Zhengguo Shen, Mujie Zhao, Rongchong Hu, Shengping Zheng, Xuyang Wang, Peng Yang, Yanjuan Front Plant Sci Plant Science The fruit peel color is an important factor that affects its quality. However, genes involved in regulating pericarp color in bottle gourd (Lagenaria siceraria) have not been explored to date. Genetic analysis of color traits in bottle gourd peel through a genetic population of six generations demonstrated that the green color of peels is inherited as a single gene dominant trait. Combined phenotype-genotype analysis of recombinant plants using BSA-seq mapped the candidate gene to a 22.645 Kb interval at the head end of chromosome 1. We observed that the final interval contained only one gene, LsAPRR2 (HG_GLEAN_10010973). Sequence and spatiotemporal expression analyses of LsAPRR2 unraveled two nonsynonymous mutations (A→G) and (G→C) in the parental CDS sequences. Further, LsAPRR2 expression was higher in all green-skinned bottle gourds (H16) at various stages of fruit development than in white-skinned bottle gourds (H06). Cloning and sequence comparison of the two parental LsAPRR2 promoter regions indicated 11 bases insertion and 8 SNPs mutations in the region -991~-1033, upstream of the start codon in white bottle gourd. Proof of GUS reporting system, Genetic variation in this fragment significantly reduced the expression of LsAPRR2 in the pericarp of white bottle gourd. In addition, we developed a tightly linked (accuracy 93.88%) InDel marker for the promoter variant segment. Overall, the current study provides a theoretical basis for comprehensive elucidation of the regulatory mechanisms underlying the determination of bottle gourd pericarp color. This would further help in the directed molecular design breeding of bottle gourd pericarp. Frontiers Media S.A. 2023-02-15 /pmc/articles/PMC9975725/ /pubmed/36875578 http://dx.doi.org/10.3389/fpls.2023.1130669 Text en Copyright © 2023 Huo, Zhang, Yu, Liu, Shen, Zhao, Hu, Zheng, Wang and Yang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Huo, Yulai
Zhang, Gui
Yu, Wenjin
Liu, Zhengguo
Shen, Mujie
Zhao, Rongchong
Hu, Shengping
Zheng, Xuyang
Wang, Peng
Yang, Yanjuan
Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria)
title Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria)
title_full Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria)
title_fullStr Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria)
title_full_unstemmed Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria)
title_short Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria)
title_sort forward genetic studies reveal lsaprr2 as a key gene in regulating the green color of pericarp in bottle gourd (lagenaria siceraria)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9975725/
https://www.ncbi.nlm.nih.gov/pubmed/36875578
http://dx.doi.org/10.3389/fpls.2023.1130669
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