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Complex scaffold remodeling in plant triterpene biosynthesis
Triterpenes with complex scaffold modifications are widespread in the plant kingdom. Limonoids are an exemplary family that are responsible for the bitter taste in citrus (e.g., limonin) and the active constituents of neem oil, a widely used bioinsecticide (e.g., azadirachtin). Despite the commercia...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9976607/ https://www.ncbi.nlm.nih.gov/pubmed/36701471 http://dx.doi.org/10.1126/science.adf1017 |
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author | De La Peña, Ricardo Hodgson, Hannah Liu, Jack Chun-Ting Stephenson, Michael J. Martin, Azahara C. Owen, Charlotte Harkess, Alex Leebens-Mack, Jim Jimenez, Luis E. Osbourn, Anne Sattely, Elizabeth S. |
author_facet | De La Peña, Ricardo Hodgson, Hannah Liu, Jack Chun-Ting Stephenson, Michael J. Martin, Azahara C. Owen, Charlotte Harkess, Alex Leebens-Mack, Jim Jimenez, Luis E. Osbourn, Anne Sattely, Elizabeth S. |
author_sort | De La Peña, Ricardo |
collection | PubMed |
description | Triterpenes with complex scaffold modifications are widespread in the plant kingdom. Limonoids are an exemplary family that are responsible for the bitter taste in citrus (e.g., limonin) and the active constituents of neem oil, a widely used bioinsecticide (e.g., azadirachtin). Despite the commercial value of limonoids, a complete biosynthetic route has not been described. Here, we report the discovery of 22 enzymes, including a pair of neofunctionalized sterol isomerases, that catalyze 12 unique reactions in the total biosynthesis of kihadalactone A and azadiraone, products that bear the signature limonoid furan. These results enable access to valuable limonoids and provide a template for discovery and reconstitution of triterpene biosynthetic pathways in plants that require multiple skeletal rearrangements and oxidations. |
format | Online Article Text |
id | pubmed-9976607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
record_format | MEDLINE/PubMed |
spelling | pubmed-99766072023-03-01 Complex scaffold remodeling in plant triterpene biosynthesis De La Peña, Ricardo Hodgson, Hannah Liu, Jack Chun-Ting Stephenson, Michael J. Martin, Azahara C. Owen, Charlotte Harkess, Alex Leebens-Mack, Jim Jimenez, Luis E. Osbourn, Anne Sattely, Elizabeth S. Science Article Triterpenes with complex scaffold modifications are widespread in the plant kingdom. Limonoids are an exemplary family that are responsible for the bitter taste in citrus (e.g., limonin) and the active constituents of neem oil, a widely used bioinsecticide (e.g., azadirachtin). Despite the commercial value of limonoids, a complete biosynthetic route has not been described. Here, we report the discovery of 22 enzymes, including a pair of neofunctionalized sterol isomerases, that catalyze 12 unique reactions in the total biosynthesis of kihadalactone A and azadiraone, products that bear the signature limonoid furan. These results enable access to valuable limonoids and provide a template for discovery and reconstitution of triterpene biosynthetic pathways in plants that require multiple skeletal rearrangements and oxidations. 2023-01-27 2023-01-26 /pmc/articles/PMC9976607/ /pubmed/36701471 http://dx.doi.org/10.1126/science.adf1017 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License, which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article De La Peña, Ricardo Hodgson, Hannah Liu, Jack Chun-Ting Stephenson, Michael J. Martin, Azahara C. Owen, Charlotte Harkess, Alex Leebens-Mack, Jim Jimenez, Luis E. Osbourn, Anne Sattely, Elizabeth S. Complex scaffold remodeling in plant triterpene biosynthesis |
title | Complex scaffold remodeling in plant triterpene biosynthesis |
title_full | Complex scaffold remodeling in plant triterpene biosynthesis |
title_fullStr | Complex scaffold remodeling in plant triterpene biosynthesis |
title_full_unstemmed | Complex scaffold remodeling in plant triterpene biosynthesis |
title_short | Complex scaffold remodeling in plant triterpene biosynthesis |
title_sort | complex scaffold remodeling in plant triterpene biosynthesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9976607/ https://www.ncbi.nlm.nih.gov/pubmed/36701471 http://dx.doi.org/10.1126/science.adf1017 |
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