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Nanopore sequencing for N1-methylpseudouridine in RNA reveals sequence-dependent discrimination of the modified nucleotide triphosphate during transcription
Direct RNA sequencing with a commercial nanopore platform was used to sequence RNA containing uridine (U), pseudouridine (Ψ) or N1-methylpseudouridine (m(1)Ψ) in >100 different 5-nucleotide contexts. The base calling data for Ψ or m(1)Ψ were similar but different from U allowing their detection....
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Oxford University Press
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9976907/ https://www.ncbi.nlm.nih.gov/pubmed/36727474 http://dx.doi.org/10.1093/nar/gkad044 |