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Functional imaging and quantification of multineuronal olfactory responses in C. elegans

Many animals perceive odorant molecules by collecting information from ensembles of olfactory neurons, where each neuron uses receptors that are tuned to recognize certain odorant molecules with different binding affinity. Olfactory systems are able, in principle, to detect and discriminate diverse...

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Autores principales: Lin, Albert, Qin, Shanshan, Casademunt, Helena, Wu, Min, Hung, Wesley, Cain, Gregory, Tan, Nicolas Z., Valenzuela, Raymond, Lesanpezeshki, Leila, Venkatachalam, Vivek, Pehlevan, Cengiz, Zhen, Mei, Samuel, Aravinthan D.T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977185/
https://www.ncbi.nlm.nih.gov/pubmed/36857454
http://dx.doi.org/10.1126/sciadv.ade1249
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author Lin, Albert
Qin, Shanshan
Casademunt, Helena
Wu, Min
Hung, Wesley
Cain, Gregory
Tan, Nicolas Z.
Valenzuela, Raymond
Lesanpezeshki, Leila
Venkatachalam, Vivek
Pehlevan, Cengiz
Zhen, Mei
Samuel, Aravinthan D.T.
author_facet Lin, Albert
Qin, Shanshan
Casademunt, Helena
Wu, Min
Hung, Wesley
Cain, Gregory
Tan, Nicolas Z.
Valenzuela, Raymond
Lesanpezeshki, Leila
Venkatachalam, Vivek
Pehlevan, Cengiz
Zhen, Mei
Samuel, Aravinthan D.T.
author_sort Lin, Albert
collection PubMed
description Many animals perceive odorant molecules by collecting information from ensembles of olfactory neurons, where each neuron uses receptors that are tuned to recognize certain odorant molecules with different binding affinity. Olfactory systems are able, in principle, to detect and discriminate diverse odorants using combinatorial coding strategies. We have combined microfluidics and multineuronal imaging to study the ensemble-level olfactory representations at the sensory periphery of the nematode Caenorhabditis elegans. The collective activity of C. elegans chemosensory neurons reveals high-dimensional representations of olfactory information across a broad space of odorant molecules. We reveal diverse tuning properties and dose-response curves across chemosensory neurons and across odorants. We describe the unique contribution of each sensory neuron to an ensemble-level code for volatile odorants. We show that a natural stimuli, a set of nematode pheromones, are also encoded by the sensory ensemble. The integrated activity of the C. elegans chemosensory neurons contains sufficient information to robustly encode the intensity and identity of diverse chemical stimuli.
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spelling pubmed-99771852023-03-02 Functional imaging and quantification of multineuronal olfactory responses in C. elegans Lin, Albert Qin, Shanshan Casademunt, Helena Wu, Min Hung, Wesley Cain, Gregory Tan, Nicolas Z. Valenzuela, Raymond Lesanpezeshki, Leila Venkatachalam, Vivek Pehlevan, Cengiz Zhen, Mei Samuel, Aravinthan D.T. Sci Adv Neuroscience Many animals perceive odorant molecules by collecting information from ensembles of olfactory neurons, where each neuron uses receptors that are tuned to recognize certain odorant molecules with different binding affinity. Olfactory systems are able, in principle, to detect and discriminate diverse odorants using combinatorial coding strategies. We have combined microfluidics and multineuronal imaging to study the ensemble-level olfactory representations at the sensory periphery of the nematode Caenorhabditis elegans. The collective activity of C. elegans chemosensory neurons reveals high-dimensional representations of olfactory information across a broad space of odorant molecules. We reveal diverse tuning properties and dose-response curves across chemosensory neurons and across odorants. We describe the unique contribution of each sensory neuron to an ensemble-level code for volatile odorants. We show that a natural stimuli, a set of nematode pheromones, are also encoded by the sensory ensemble. The integrated activity of the C. elegans chemosensory neurons contains sufficient information to robustly encode the intensity and identity of diverse chemical stimuli. American Association for the Advancement of Science 2023-03-01 /pmc/articles/PMC9977185/ /pubmed/36857454 http://dx.doi.org/10.1126/sciadv.ade1249 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Neuroscience
Lin, Albert
Qin, Shanshan
Casademunt, Helena
Wu, Min
Hung, Wesley
Cain, Gregory
Tan, Nicolas Z.
Valenzuela, Raymond
Lesanpezeshki, Leila
Venkatachalam, Vivek
Pehlevan, Cengiz
Zhen, Mei
Samuel, Aravinthan D.T.
Functional imaging and quantification of multineuronal olfactory responses in C. elegans
title Functional imaging and quantification of multineuronal olfactory responses in C. elegans
title_full Functional imaging and quantification of multineuronal olfactory responses in C. elegans
title_fullStr Functional imaging and quantification of multineuronal olfactory responses in C. elegans
title_full_unstemmed Functional imaging and quantification of multineuronal olfactory responses in C. elegans
title_short Functional imaging and quantification of multineuronal olfactory responses in C. elegans
title_sort functional imaging and quantification of multineuronal olfactory responses in c. elegans
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977185/
https://www.ncbi.nlm.nih.gov/pubmed/36857454
http://dx.doi.org/10.1126/sciadv.ade1249
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