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Functional imaging and quantification of multineuronal olfactory responses in C. elegans
Many animals perceive odorant molecules by collecting information from ensembles of olfactory neurons, where each neuron uses receptors that are tuned to recognize certain odorant molecules with different binding affinity. Olfactory systems are able, in principle, to detect and discriminate diverse...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977185/ https://www.ncbi.nlm.nih.gov/pubmed/36857454 http://dx.doi.org/10.1126/sciadv.ade1249 |
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author | Lin, Albert Qin, Shanshan Casademunt, Helena Wu, Min Hung, Wesley Cain, Gregory Tan, Nicolas Z. Valenzuela, Raymond Lesanpezeshki, Leila Venkatachalam, Vivek Pehlevan, Cengiz Zhen, Mei Samuel, Aravinthan D.T. |
author_facet | Lin, Albert Qin, Shanshan Casademunt, Helena Wu, Min Hung, Wesley Cain, Gregory Tan, Nicolas Z. Valenzuela, Raymond Lesanpezeshki, Leila Venkatachalam, Vivek Pehlevan, Cengiz Zhen, Mei Samuel, Aravinthan D.T. |
author_sort | Lin, Albert |
collection | PubMed |
description | Many animals perceive odorant molecules by collecting information from ensembles of olfactory neurons, where each neuron uses receptors that are tuned to recognize certain odorant molecules with different binding affinity. Olfactory systems are able, in principle, to detect and discriminate diverse odorants using combinatorial coding strategies. We have combined microfluidics and multineuronal imaging to study the ensemble-level olfactory representations at the sensory periphery of the nematode Caenorhabditis elegans. The collective activity of C. elegans chemosensory neurons reveals high-dimensional representations of olfactory information across a broad space of odorant molecules. We reveal diverse tuning properties and dose-response curves across chemosensory neurons and across odorants. We describe the unique contribution of each sensory neuron to an ensemble-level code for volatile odorants. We show that a natural stimuli, a set of nematode pheromones, are also encoded by the sensory ensemble. The integrated activity of the C. elegans chemosensory neurons contains sufficient information to robustly encode the intensity and identity of diverse chemical stimuli. |
format | Online Article Text |
id | pubmed-9977185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-99771852023-03-02 Functional imaging and quantification of multineuronal olfactory responses in C. elegans Lin, Albert Qin, Shanshan Casademunt, Helena Wu, Min Hung, Wesley Cain, Gregory Tan, Nicolas Z. Valenzuela, Raymond Lesanpezeshki, Leila Venkatachalam, Vivek Pehlevan, Cengiz Zhen, Mei Samuel, Aravinthan D.T. Sci Adv Neuroscience Many animals perceive odorant molecules by collecting information from ensembles of olfactory neurons, where each neuron uses receptors that are tuned to recognize certain odorant molecules with different binding affinity. Olfactory systems are able, in principle, to detect and discriminate diverse odorants using combinatorial coding strategies. We have combined microfluidics and multineuronal imaging to study the ensemble-level olfactory representations at the sensory periphery of the nematode Caenorhabditis elegans. The collective activity of C. elegans chemosensory neurons reveals high-dimensional representations of olfactory information across a broad space of odorant molecules. We reveal diverse tuning properties and dose-response curves across chemosensory neurons and across odorants. We describe the unique contribution of each sensory neuron to an ensemble-level code for volatile odorants. We show that a natural stimuli, a set of nematode pheromones, are also encoded by the sensory ensemble. The integrated activity of the C. elegans chemosensory neurons contains sufficient information to robustly encode the intensity and identity of diverse chemical stimuli. American Association for the Advancement of Science 2023-03-01 /pmc/articles/PMC9977185/ /pubmed/36857454 http://dx.doi.org/10.1126/sciadv.ade1249 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Neuroscience Lin, Albert Qin, Shanshan Casademunt, Helena Wu, Min Hung, Wesley Cain, Gregory Tan, Nicolas Z. Valenzuela, Raymond Lesanpezeshki, Leila Venkatachalam, Vivek Pehlevan, Cengiz Zhen, Mei Samuel, Aravinthan D.T. Functional imaging and quantification of multineuronal olfactory responses in C. elegans |
title | Functional imaging and quantification of multineuronal olfactory responses in C. elegans |
title_full | Functional imaging and quantification of multineuronal olfactory responses in C. elegans |
title_fullStr | Functional imaging and quantification of multineuronal olfactory responses in C. elegans |
title_full_unstemmed | Functional imaging and quantification of multineuronal olfactory responses in C. elegans |
title_short | Functional imaging and quantification of multineuronal olfactory responses in C. elegans |
title_sort | functional imaging and quantification of multineuronal olfactory responses in c. elegans |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977185/ https://www.ncbi.nlm.nih.gov/pubmed/36857454 http://dx.doi.org/10.1126/sciadv.ade1249 |
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