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Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes
High-grade serous ovarian cancer (HGSOC) metastasizes through transcoelomic spread, with both single cells and spheroids of tumor cells observed in patient ascites. These spheroids may form through single cells that detach and aggregate (Sph-SC) or through collective detachment (Sph-CD). We develope...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977464/ https://www.ncbi.nlm.nih.gov/pubmed/36875739 http://dx.doi.org/10.1063/5.0132254 |
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author | Micek, Hannah M. Rosenstock, Lauren Ma, Yicheng Hielsberg, Caitlin Montemorano, Lauren Gari, Metti K. Ponik, Suzanne M. Kreeger, Pamela K. |
author_facet | Micek, Hannah M. Rosenstock, Lauren Ma, Yicheng Hielsberg, Caitlin Montemorano, Lauren Gari, Metti K. Ponik, Suzanne M. Kreeger, Pamela K. |
author_sort | Micek, Hannah M. |
collection | PubMed |
description | High-grade serous ovarian cancer (HGSOC) metastasizes through transcoelomic spread, with both single cells and spheroids of tumor cells observed in patient ascites. These spheroids may form through single cells that detach and aggregate (Sph-SC) or through collective detachment (Sph-CD). We developed an in vitro model to generate and separate Sph-SC from Sph-CD to enable study of Sph-CD in disease progression. In vitro-generated Sph-CD and spheroids isolated from ascites were similar in size (mean diameter 51 vs 55 μm, p > 0.05) and incorporated multiple ECM proteins. Using the in vitro model, nascent protein labeling, and qRT-PCR, we determined that ECM was produced after detachment. As fibronectin plays a key role in many cell adhesion events, we confirmed that inhibiting RGD-based adhesion or fibronectin assembly reduced Sph-CD-mesothelial adhesion strength under shear stress. Our model will enable future studies to determine factors that favor formation of Sph-CD, as well as allow investigators to manipulate Sph-CD to better study their effects on HGSOC progression. |
format | Online Article Text |
id | pubmed-9977464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-99774642023-03-02 Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes Micek, Hannah M. Rosenstock, Lauren Ma, Yicheng Hielsberg, Caitlin Montemorano, Lauren Gari, Metti K. Ponik, Suzanne M. Kreeger, Pamela K. APL Bioeng Articles High-grade serous ovarian cancer (HGSOC) metastasizes through transcoelomic spread, with both single cells and spheroids of tumor cells observed in patient ascites. These spheroids may form through single cells that detach and aggregate (Sph-SC) or through collective detachment (Sph-CD). We developed an in vitro model to generate and separate Sph-SC from Sph-CD to enable study of Sph-CD in disease progression. In vitro-generated Sph-CD and spheroids isolated from ascites were similar in size (mean diameter 51 vs 55 μm, p > 0.05) and incorporated multiple ECM proteins. Using the in vitro model, nascent protein labeling, and qRT-PCR, we determined that ECM was produced after detachment. As fibronectin plays a key role in many cell adhesion events, we confirmed that inhibiting RGD-based adhesion or fibronectin assembly reduced Sph-CD-mesothelial adhesion strength under shear stress. Our model will enable future studies to determine factors that favor formation of Sph-CD, as well as allow investigators to manipulate Sph-CD to better study their effects on HGSOC progression. AIP Publishing LLC 2023-02-28 /pmc/articles/PMC9977464/ /pubmed/36875739 http://dx.doi.org/10.1063/5.0132254 Text en © 2023 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Articles Micek, Hannah M. Rosenstock, Lauren Ma, Yicheng Hielsberg, Caitlin Montemorano, Lauren Gari, Metti K. Ponik, Suzanne M. Kreeger, Pamela K. Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes |
title | Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes |
title_full | Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes |
title_fullStr | Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes |
title_full_unstemmed | Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes |
title_short | Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes |
title_sort | model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ecm to support metastatic processes |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977464/ https://www.ncbi.nlm.nih.gov/pubmed/36875739 http://dx.doi.org/10.1063/5.0132254 |
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