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Identification of B and T Cell Epitopes to Design an Epitope-Based Peptide Vaccine against the Cell Surface Binding Protein of Monkeypox Virus: An Immunoinformatics Study
BACKGROUND: Although the monkeypox virus-associated illness was previously confined to Africa, recently, it has started to spread across the globe and become a significant threat to human lives. Hence, this study was designed to identify the B and T cell epitopes and develop an epitope-based peptide...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977553/ https://www.ncbi.nlm.nih.gov/pubmed/36874624 http://dx.doi.org/10.1155/2023/2274415 |
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author | Mazumder, Lincon Hasan, Md. Rakibul Fatema, Kanij Begum, Shamima Azad, Abul Kalam Islam, Mohammad Ariful |
author_facet | Mazumder, Lincon Hasan, Md. Rakibul Fatema, Kanij Begum, Shamima Azad, Abul Kalam Islam, Mohammad Ariful |
author_sort | Mazumder, Lincon |
collection | PubMed |
description | BACKGROUND: Although the monkeypox virus-associated illness was previously confined to Africa, recently, it has started to spread across the globe and become a significant threat to human lives. Hence, this study was designed to identify the B and T cell epitopes and develop an epitope-based peptide vaccine against this virus's cell surface binding protein through an in silico approach to combat monkeypox-associated diseases. RESULTS: The analysis revealed that the cell surface binding protein of the monkeypox virus contains 30 B cell and 19 T cell epitopes within the given parameter. Among the T cell epitopes, epitope “ILFLMSQRY” was found to be one of the most potential peptide vaccine candidates. The docking analysis revealed an excellent binding affinity of this epitope with the human receptor HLA-B(∗)15:01 with a very low binding energy (-7.5 kcal/mol). CONCLUSION: The outcome of this research will aid the development of a T cell epitope-based peptide vaccine, and the discovered B and T cell epitopes will facilitate the creation of other epitope and multi-epitope-based vaccines in the future. This research will also serve as a basis for further in vitro and in vivo analysis to develop a vaccine that is effective against the monkeypox virus. |
format | Online Article Text |
id | pubmed-9977553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-99775532023-03-02 Identification of B and T Cell Epitopes to Design an Epitope-Based Peptide Vaccine against the Cell Surface Binding Protein of Monkeypox Virus: An Immunoinformatics Study Mazumder, Lincon Hasan, Md. Rakibul Fatema, Kanij Begum, Shamima Azad, Abul Kalam Islam, Mohammad Ariful J Immunol Res Research Article BACKGROUND: Although the monkeypox virus-associated illness was previously confined to Africa, recently, it has started to spread across the globe and become a significant threat to human lives. Hence, this study was designed to identify the B and T cell epitopes and develop an epitope-based peptide vaccine against this virus's cell surface binding protein through an in silico approach to combat monkeypox-associated diseases. RESULTS: The analysis revealed that the cell surface binding protein of the monkeypox virus contains 30 B cell and 19 T cell epitopes within the given parameter. Among the T cell epitopes, epitope “ILFLMSQRY” was found to be one of the most potential peptide vaccine candidates. The docking analysis revealed an excellent binding affinity of this epitope with the human receptor HLA-B(∗)15:01 with a very low binding energy (-7.5 kcal/mol). CONCLUSION: The outcome of this research will aid the development of a T cell epitope-based peptide vaccine, and the discovered B and T cell epitopes will facilitate the creation of other epitope and multi-epitope-based vaccines in the future. This research will also serve as a basis for further in vitro and in vivo analysis to develop a vaccine that is effective against the monkeypox virus. Hindawi 2023-02-22 /pmc/articles/PMC9977553/ /pubmed/36874624 http://dx.doi.org/10.1155/2023/2274415 Text en Copyright © 2023 Lincon Mazumder et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mazumder, Lincon Hasan, Md. Rakibul Fatema, Kanij Begum, Shamima Azad, Abul Kalam Islam, Mohammad Ariful Identification of B and T Cell Epitopes to Design an Epitope-Based Peptide Vaccine against the Cell Surface Binding Protein of Monkeypox Virus: An Immunoinformatics Study |
title | Identification of B and T Cell Epitopes to Design an Epitope-Based Peptide Vaccine against the Cell Surface Binding Protein of Monkeypox Virus: An Immunoinformatics Study |
title_full | Identification of B and T Cell Epitopes to Design an Epitope-Based Peptide Vaccine against the Cell Surface Binding Protein of Monkeypox Virus: An Immunoinformatics Study |
title_fullStr | Identification of B and T Cell Epitopes to Design an Epitope-Based Peptide Vaccine against the Cell Surface Binding Protein of Monkeypox Virus: An Immunoinformatics Study |
title_full_unstemmed | Identification of B and T Cell Epitopes to Design an Epitope-Based Peptide Vaccine against the Cell Surface Binding Protein of Monkeypox Virus: An Immunoinformatics Study |
title_short | Identification of B and T Cell Epitopes to Design an Epitope-Based Peptide Vaccine against the Cell Surface Binding Protein of Monkeypox Virus: An Immunoinformatics Study |
title_sort | identification of b and t cell epitopes to design an epitope-based peptide vaccine against the cell surface binding protein of monkeypox virus: an immunoinformatics study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977553/ https://www.ncbi.nlm.nih.gov/pubmed/36874624 http://dx.doi.org/10.1155/2023/2274415 |
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