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Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation

Background: With an increasing number of patients experiencing infertility due to chronic salpingitis after Chlamydia trachomatis (CT) infection, there is an unmet need for tissue repair or regeneration therapies. Treatment with human umbilical cord mesenchymal stem cell-derived extracellular vesicl...

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Autores principales: Zhang, Changlin, Liao, Wei, Li, Weizhao, Li, Mengxiong, Xu, Xiaoyu, Sun, Haohui, Xue, Yaohua, Liu, Lixiang, Qiu, Jiehong, Zhang, Chi, Zhang, Xunzhi, Ye, Juntong, Du, Jingran, Deng, David Y. B., Deng, Wuguo, Li, Tian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977816/
https://www.ncbi.nlm.nih.gov/pubmed/36875046
http://dx.doi.org/10.3389/fphys.2023.1131701
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author Zhang, Changlin
Liao, Wei
Li, Weizhao
Li, Mengxiong
Xu, Xiaoyu
Sun, Haohui
Xue, Yaohua
Liu, Lixiang
Qiu, Jiehong
Zhang, Chi
Zhang, Xunzhi
Ye, Juntong
Du, Jingran
Deng, David Y. B.
Deng, Wuguo
Li, Tian
author_facet Zhang, Changlin
Liao, Wei
Li, Weizhao
Li, Mengxiong
Xu, Xiaoyu
Sun, Haohui
Xue, Yaohua
Liu, Lixiang
Qiu, Jiehong
Zhang, Chi
Zhang, Xunzhi
Ye, Juntong
Du, Jingran
Deng, David Y. B.
Deng, Wuguo
Li, Tian
author_sort Zhang, Changlin
collection PubMed
description Background: With an increasing number of patients experiencing infertility due to chronic salpingitis after Chlamydia trachomatis (CT) infection, there is an unmet need for tissue repair or regeneration therapies. Treatment with human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hucMSC-EV) provides an attractive cell-free therapeutic approach. Methods: In this study, we investigated the alleviating effect of hucMSC-EV on tubal inflammatory infertility caused by CT using in vivo animal experiments. Furthermore, we examined the effect of hucMSC-EV on inducing macrophage polarization to explore the molecular mechanism. Results: Our results showed that tubal inflammatory infertility caused by Chlamydia infection was significantly alleviated in the hucMSC-EV treatment group compared with the control group. Further mechanistic experiments showed that the application of hucMSC-EV induced macrophage polarization from the M1 to the M2 type via the NF-κB signaling pathway, improved the local inflammatory microenvironment of fallopian tubes and inhibited tube inflammation. Conclusion: We conclude that this approach represents a promising cell-free avenue to ameliorate infertility due to chronic salpingitis.
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spelling pubmed-99778162023-03-03 Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation Zhang, Changlin Liao, Wei Li, Weizhao Li, Mengxiong Xu, Xiaoyu Sun, Haohui Xue, Yaohua Liu, Lixiang Qiu, Jiehong Zhang, Chi Zhang, Xunzhi Ye, Juntong Du, Jingran Deng, David Y. B. Deng, Wuguo Li, Tian Front Physiol Physiology Background: With an increasing number of patients experiencing infertility due to chronic salpingitis after Chlamydia trachomatis (CT) infection, there is an unmet need for tissue repair or regeneration therapies. Treatment with human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hucMSC-EV) provides an attractive cell-free therapeutic approach. Methods: In this study, we investigated the alleviating effect of hucMSC-EV on tubal inflammatory infertility caused by CT using in vivo animal experiments. Furthermore, we examined the effect of hucMSC-EV on inducing macrophage polarization to explore the molecular mechanism. Results: Our results showed that tubal inflammatory infertility caused by Chlamydia infection was significantly alleviated in the hucMSC-EV treatment group compared with the control group. Further mechanistic experiments showed that the application of hucMSC-EV induced macrophage polarization from the M1 to the M2 type via the NF-κB signaling pathway, improved the local inflammatory microenvironment of fallopian tubes and inhibited tube inflammation. Conclusion: We conclude that this approach represents a promising cell-free avenue to ameliorate infertility due to chronic salpingitis. Frontiers Media S.A. 2023-02-16 /pmc/articles/PMC9977816/ /pubmed/36875046 http://dx.doi.org/10.3389/fphys.2023.1131701 Text en Copyright © 2023 Zhang, Liao, Li, Li, Xu, Sun, Xue, Liu, Qiu, Zhang, Zhang, Ye, Du, Deng, Deng and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Zhang, Changlin
Liao, Wei
Li, Weizhao
Li, Mengxiong
Xu, Xiaoyu
Sun, Haohui
Xue, Yaohua
Liu, Lixiang
Qiu, Jiehong
Zhang, Chi
Zhang, Xunzhi
Ye, Juntong
Du, Jingran
Deng, David Y. B.
Deng, Wuguo
Li, Tian
Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation
title Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation
title_full Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation
title_fullStr Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation
title_full_unstemmed Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation
title_short Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation
title_sort human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting m1–to–m2 transformation
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977816/
https://www.ncbi.nlm.nih.gov/pubmed/36875046
http://dx.doi.org/10.3389/fphys.2023.1131701
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