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Distribution of dietary nitrate and its metabolites in rat tissues after (15)N-labeled nitrate administration
The reduction pathway of nitrate (NO(3)(−)) and nitrite (NO(2)(−)) to nitric oxide (NO) contributes to regulating many physiological processes. To examine the rate and extent of dietary nitrate absorption and its reduction to nitrite, we supplemented rat diets with Na(15)NO(3)-containing water (1 g/...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977953/ https://www.ncbi.nlm.nih.gov/pubmed/36859526 http://dx.doi.org/10.1038/s41598-023-28190-2 |
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author | Park, Ji Won Piknova, Barbora Walter, Peter J. Cai, Hongyi Upanan, Supranee Thomas, Samantha M. Tunau-Spencer, Khalid J. Schechter, Alan N. |
author_facet | Park, Ji Won Piknova, Barbora Walter, Peter J. Cai, Hongyi Upanan, Supranee Thomas, Samantha M. Tunau-Spencer, Khalid J. Schechter, Alan N. |
author_sort | Park, Ji Won |
collection | PubMed |
description | The reduction pathway of nitrate (NO(3)(−)) and nitrite (NO(2)(−)) to nitric oxide (NO) contributes to regulating many physiological processes. To examine the rate and extent of dietary nitrate absorption and its reduction to nitrite, we supplemented rat diets with Na(15)NO(3)-containing water (1 g/L) and collected plasma, urine and several tissue samples. We found that plasma and urine showed 8.8- and 11.7-fold increases respectively in total nitrate concentrations in 1-day supplementation group compared to control. In tissue samples—gluteus, liver and eyes—we found 1.7-, 2.4- and 4.2-fold increases respectively in 1-day supplementation group. These increases remained similar in 3-day supplementation group. LC–MS/MS analysis showed that the augmented nitrate concentrations were primarily from the exogenously provided (15)N-nitrate. Overall nitrite concentrations and percent of (15)N-nitrite were also greatly increased in all samples after nitrate supplementation; eye homogenates showed larger increases compared to gluteus and liver. Moreover, genes related to nitrate transport and reduction (Sialin, CLC and XOR) were upregulated after nitrate supplementation for 3 days in muscle (Sialin 2.3-, CLC1 1.3-, CLC3 2.1-, XOR 2.4-fold) and eye (XOR 1.7-fold) homogenates. These results demonstrate that dietary nitrate is quickly absorbed into circulation and tissues, and it can be reduced to nitrite in tissues (and likely to NO) suggesting that nitrate-enriched diets can be an efficient intervention to enhance nitrite and NO bioavailability. |
format | Online Article Text |
id | pubmed-9977953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-99779532023-03-03 Distribution of dietary nitrate and its metabolites in rat tissues after (15)N-labeled nitrate administration Park, Ji Won Piknova, Barbora Walter, Peter J. Cai, Hongyi Upanan, Supranee Thomas, Samantha M. Tunau-Spencer, Khalid J. Schechter, Alan N. Sci Rep Article The reduction pathway of nitrate (NO(3)(−)) and nitrite (NO(2)(−)) to nitric oxide (NO) contributes to regulating many physiological processes. To examine the rate and extent of dietary nitrate absorption and its reduction to nitrite, we supplemented rat diets with Na(15)NO(3)-containing water (1 g/L) and collected plasma, urine and several tissue samples. We found that plasma and urine showed 8.8- and 11.7-fold increases respectively in total nitrate concentrations in 1-day supplementation group compared to control. In tissue samples—gluteus, liver and eyes—we found 1.7-, 2.4- and 4.2-fold increases respectively in 1-day supplementation group. These increases remained similar in 3-day supplementation group. LC–MS/MS analysis showed that the augmented nitrate concentrations were primarily from the exogenously provided (15)N-nitrate. Overall nitrite concentrations and percent of (15)N-nitrite were also greatly increased in all samples after nitrate supplementation; eye homogenates showed larger increases compared to gluteus and liver. Moreover, genes related to nitrate transport and reduction (Sialin, CLC and XOR) were upregulated after nitrate supplementation for 3 days in muscle (Sialin 2.3-, CLC1 1.3-, CLC3 2.1-, XOR 2.4-fold) and eye (XOR 1.7-fold) homogenates. These results demonstrate that dietary nitrate is quickly absorbed into circulation and tissues, and it can be reduced to nitrite in tissues (and likely to NO) suggesting that nitrate-enriched diets can be an efficient intervention to enhance nitrite and NO bioavailability. Nature Publishing Group UK 2023-03-01 /pmc/articles/PMC9977953/ /pubmed/36859526 http://dx.doi.org/10.1038/s41598-023-28190-2 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Park, Ji Won Piknova, Barbora Walter, Peter J. Cai, Hongyi Upanan, Supranee Thomas, Samantha M. Tunau-Spencer, Khalid J. Schechter, Alan N. Distribution of dietary nitrate and its metabolites in rat tissues after (15)N-labeled nitrate administration |
title | Distribution of dietary nitrate and its metabolites in rat tissues after (15)N-labeled nitrate administration |
title_full | Distribution of dietary nitrate and its metabolites in rat tissues after (15)N-labeled nitrate administration |
title_fullStr | Distribution of dietary nitrate and its metabolites in rat tissues after (15)N-labeled nitrate administration |
title_full_unstemmed | Distribution of dietary nitrate and its metabolites in rat tissues after (15)N-labeled nitrate administration |
title_short | Distribution of dietary nitrate and its metabolites in rat tissues after (15)N-labeled nitrate administration |
title_sort | distribution of dietary nitrate and its metabolites in rat tissues after (15)n-labeled nitrate administration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977953/ https://www.ncbi.nlm.nih.gov/pubmed/36859526 http://dx.doi.org/10.1038/s41598-023-28190-2 |
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