A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue

The detection of neurotransmitters has extensively been applied to the study of the pathogenesis, diagnosis, and therapeutic effect of drugs on many neuropsychiatric diseases. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been employed to determine neurotransmitter...

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Autores principales: Dai, Di, Qin, Qian, Zhu, Xinyu, Fang, Qiuyuan, Meng, Xianzong, Bai, Lin, Yang, Guang, Shan, Ling, Liu, Chunqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978030/
https://www.ncbi.nlm.nih.gov/pubmed/36875344
http://dx.doi.org/10.1016/j.mex.2023.102083
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author Dai, Di
Qin, Qian
Zhu, Xinyu
Fang, Qiuyuan
Meng, Xianzong
Bai, Lin
Yang, Guang
Shan, Ling
Liu, Chunqing
author_facet Dai, Di
Qin, Qian
Zhu, Xinyu
Fang, Qiuyuan
Meng, Xianzong
Bai, Lin
Yang, Guang
Shan, Ling
Liu, Chunqing
author_sort Dai, Di
collection PubMed
description The detection of neurotransmitters has extensively been applied to the study of the pathogenesis, diagnosis, and therapeutic effect of drugs on many neuropsychiatric diseases. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been employed to determine neurotransmitters levels due to its distinct advantages. However, neurotransmitter detection still presents some challenges. A rapid and sensitive HPLC-MS/MS protocol has been established in our lab, which can simultaneously detect 5 neurotransmitters with an easy pretreatment procedure. The protocol provides demanded reference value for the lab using an Agilent HPLC-MS/MS system with a triple quadrupole analyzer.
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spelling pubmed-99780302023-03-03 A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue Dai, Di Qin, Qian Zhu, Xinyu Fang, Qiuyuan Meng, Xianzong Bai, Lin Yang, Guang Shan, Ling Liu, Chunqing MethodsX Protocol Article The detection of neurotransmitters has extensively been applied to the study of the pathogenesis, diagnosis, and therapeutic effect of drugs on many neuropsychiatric diseases. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been employed to determine neurotransmitters levels due to its distinct advantages. However, neurotransmitter detection still presents some challenges. A rapid and sensitive HPLC-MS/MS protocol has been established in our lab, which can simultaneously detect 5 neurotransmitters with an easy pretreatment procedure. The protocol provides demanded reference value for the lab using an Agilent HPLC-MS/MS system with a triple quadrupole analyzer. Elsevier 2023-02-18 /pmc/articles/PMC9978030/ /pubmed/36875344 http://dx.doi.org/10.1016/j.mex.2023.102083 Text en © 2023 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol Article
Dai, Di
Qin, Qian
Zhu, Xinyu
Fang, Qiuyuan
Meng, Xianzong
Bai, Lin
Yang, Guang
Shan, Ling
Liu, Chunqing
A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue
title A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue
title_full A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue
title_fullStr A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue
title_full_unstemmed A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue
title_short A high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue
title_sort high performance liquid chromatography tandem mass spectrometry protocol for detection of neurotransmitters in the rat brain tissue
topic Protocol Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978030/
https://www.ncbi.nlm.nih.gov/pubmed/36875344
http://dx.doi.org/10.1016/j.mex.2023.102083
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