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A Quantitative Method for the Study of HIV-1 and Mycobacterium tuberculosis Coinfection

Mycobacterium tuberculosis and human immunodeficiency virus-1 (HIV-1) syndemic interactions are a major global health concern. Despite the clinical significance of coinfection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of coinfection is limited. Her...

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Autores principales: Donnellan, Samantha, Pennington, Shaun H, Ruggiero, Alessandra, Martinez-Rodriguez, Carmen, Pouget, Marion, Thomas, Jordan, Ward, Steve A, Pollakis, Georgios, Biagini, Giancarlo A, Paxton, William A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978310/
https://www.ncbi.nlm.nih.gov/pubmed/36537213
http://dx.doi.org/10.1093/infdis/jiac491
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author Donnellan, Samantha
Pennington, Shaun H
Ruggiero, Alessandra
Martinez-Rodriguez, Carmen
Pouget, Marion
Thomas, Jordan
Ward, Steve A
Pollakis, Georgios
Biagini, Giancarlo A
Paxton, William A
author_facet Donnellan, Samantha
Pennington, Shaun H
Ruggiero, Alessandra
Martinez-Rodriguez, Carmen
Pouget, Marion
Thomas, Jordan
Ward, Steve A
Pollakis, Georgios
Biagini, Giancarlo A
Paxton, William A
author_sort Donnellan, Samantha
collection PubMed
description Mycobacterium tuberculosis and human immunodeficiency virus-1 (HIV-1) syndemic interactions are a major global health concern. Despite the clinical significance of coinfection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of coinfection is limited. Here, we use single-round infectious HIV-1 pseudotyped viral particles expressing green fluorescent protein alongside M. tuberculosis expressing mCherry to study pathogenesis and treatment. We report that HIV-1 infection inhibited intracellular replication of M. tuberculosis and demonstrate the therapeutic activity of antiviral treatment (efavirenz) and antimicrobial treatment (rifampicin). The described method could be applied for detailed mechanistic studies to inform the development of novel treatment strategies.
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spelling pubmed-99783102023-03-03 A Quantitative Method for the Study of HIV-1 and Mycobacterium tuberculosis Coinfection Donnellan, Samantha Pennington, Shaun H Ruggiero, Alessandra Martinez-Rodriguez, Carmen Pouget, Marion Thomas, Jordan Ward, Steve A Pollakis, Georgios Biagini, Giancarlo A Paxton, William A J Infect Dis Brief Report Mycobacterium tuberculosis and human immunodeficiency virus-1 (HIV-1) syndemic interactions are a major global health concern. Despite the clinical significance of coinfection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of coinfection is limited. Here, we use single-round infectious HIV-1 pseudotyped viral particles expressing green fluorescent protein alongside M. tuberculosis expressing mCherry to study pathogenesis and treatment. We report that HIV-1 infection inhibited intracellular replication of M. tuberculosis and demonstrate the therapeutic activity of antiviral treatment (efavirenz) and antimicrobial treatment (rifampicin). The described method could be applied for detailed mechanistic studies to inform the development of novel treatment strategies. Oxford University Press 2022-12-20 /pmc/articles/PMC9978310/ /pubmed/36537213 http://dx.doi.org/10.1093/infdis/jiac491 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Report
Donnellan, Samantha
Pennington, Shaun H
Ruggiero, Alessandra
Martinez-Rodriguez, Carmen
Pouget, Marion
Thomas, Jordan
Ward, Steve A
Pollakis, Georgios
Biagini, Giancarlo A
Paxton, William A
A Quantitative Method for the Study of HIV-1 and Mycobacterium tuberculosis Coinfection
title A Quantitative Method for the Study of HIV-1 and Mycobacterium tuberculosis Coinfection
title_full A Quantitative Method for the Study of HIV-1 and Mycobacterium tuberculosis Coinfection
title_fullStr A Quantitative Method for the Study of HIV-1 and Mycobacterium tuberculosis Coinfection
title_full_unstemmed A Quantitative Method for the Study of HIV-1 and Mycobacterium tuberculosis Coinfection
title_short A Quantitative Method for the Study of HIV-1 and Mycobacterium tuberculosis Coinfection
title_sort quantitative method for the study of hiv-1 and mycobacterium tuberculosis coinfection
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978310/
https://www.ncbi.nlm.nih.gov/pubmed/36537213
http://dx.doi.org/10.1093/infdis/jiac491
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