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Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate

The Calvin-Benson-Bassham (CBB) cycle comprises the metabolic phase of photosynthesis and is responsible for carbon fixation and the production of sugar phosphates. The first step of the cycle involves the enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) which catalyzes the incorpora...

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Autores principales: Meloni, Maria, Gurrieri, Libero, Fermani, Simona, Velie, Lauren, Sparla, Francesca, Crozet, Pierre, Henri, Julien, Zaffagnini, Mirko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978339/
https://www.ncbi.nlm.nih.gov/pubmed/36875598
http://dx.doi.org/10.3389/fpls.2023.1130430
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author Meloni, Maria
Gurrieri, Libero
Fermani, Simona
Velie, Lauren
Sparla, Francesca
Crozet, Pierre
Henri, Julien
Zaffagnini, Mirko
author_facet Meloni, Maria
Gurrieri, Libero
Fermani, Simona
Velie, Lauren
Sparla, Francesca
Crozet, Pierre
Henri, Julien
Zaffagnini, Mirko
author_sort Meloni, Maria
collection PubMed
description The Calvin-Benson-Bassham (CBB) cycle comprises the metabolic phase of photosynthesis and is responsible for carbon fixation and the production of sugar phosphates. The first step of the cycle involves the enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) which catalyzes the incorporation of inorganic carbon into 3-phosphoglyceric acid (3PGA). The following steps include ten enzymes that catalyze the regeneration of ribulose-1,5-bisphosphate (RuBP), the substrate of Rubisco. While it is well established that Rubisco activity acts as a limiting step of the cycle, recent modeling studies and experimental evidence have shown that the efficiency of the pathway is also impacted by the regeneration of the Rubisco substrate itself. In this work, we review the current understanding of the structural and catalytic features of the photosynthetic enzymes that catalyze the last three steps of the regeneration phase, namely ribose-5-phosphate isomerase (RPI), ribulose-5-phosphate epimerase (RPE), and phosphoribulokinase (PRK). In addition, the redox- and metabolic-based regulatory mechanisms targeting the three enzymes are also discussed. Overall, this review highlights the importance of understudied steps in the CBB cycle and provides direction for future research aimed at improving plant productivity.
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spelling pubmed-99783392023-03-03 Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate Meloni, Maria Gurrieri, Libero Fermani, Simona Velie, Lauren Sparla, Francesca Crozet, Pierre Henri, Julien Zaffagnini, Mirko Front Plant Sci Plant Science The Calvin-Benson-Bassham (CBB) cycle comprises the metabolic phase of photosynthesis and is responsible for carbon fixation and the production of sugar phosphates. The first step of the cycle involves the enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) which catalyzes the incorporation of inorganic carbon into 3-phosphoglyceric acid (3PGA). The following steps include ten enzymes that catalyze the regeneration of ribulose-1,5-bisphosphate (RuBP), the substrate of Rubisco. While it is well established that Rubisco activity acts as a limiting step of the cycle, recent modeling studies and experimental evidence have shown that the efficiency of the pathway is also impacted by the regeneration of the Rubisco substrate itself. In this work, we review the current understanding of the structural and catalytic features of the photosynthetic enzymes that catalyze the last three steps of the regeneration phase, namely ribose-5-phosphate isomerase (RPI), ribulose-5-phosphate epimerase (RPE), and phosphoribulokinase (PRK). In addition, the redox- and metabolic-based regulatory mechanisms targeting the three enzymes are also discussed. Overall, this review highlights the importance of understudied steps in the CBB cycle and provides direction for future research aimed at improving plant productivity. Frontiers Media S.A. 2023-02-16 /pmc/articles/PMC9978339/ /pubmed/36875598 http://dx.doi.org/10.3389/fpls.2023.1130430 Text en Copyright © 2023 Meloni, Gurrieri, Fermani, Velie, Sparla, Crozet, Henri and Zaffagnini https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Meloni, Maria
Gurrieri, Libero
Fermani, Simona
Velie, Lauren
Sparla, Francesca
Crozet, Pierre
Henri, Julien
Zaffagnini, Mirko
Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate
title Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate
title_full Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate
title_fullStr Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate
title_full_unstemmed Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate
title_short Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate
title_sort ribulose-1,5-bisphosphate regeneration in the calvin-benson-bassham cycle: focus on the last three enzymatic steps that allow the formation of rubisco substrate
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978339/
https://www.ncbi.nlm.nih.gov/pubmed/36875598
http://dx.doi.org/10.3389/fpls.2023.1130430
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