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Function-driven design of Bacillus kochii and Filobasidium magnum co-culture to improve quality of flue-cured tobacco

Flue-cured tobacco (FCT) is an economical raw material whose quality affects the quality and cost of the derived product. However, the time-consuming and inefficient spontaneous aging is the primary process for improving the FCT quality in the industry. In this study, a function-driven co-culture wi...

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Autores principales: Wu, Xinying, Cai, Wen, Zhu, Pengcheng, Peng, Zheng, Zheng, Tianfei, Li, Dongliang, Li, Jianghua, Zhou, Guanyu, Zhang, Juan, Du, Guocheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978371/
https://www.ncbi.nlm.nih.gov/pubmed/36875537
http://dx.doi.org/10.3389/fmicb.2022.1024005
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author Wu, Xinying
Cai, Wen
Zhu, Pengcheng
Peng, Zheng
Zheng, Tianfei
Li, Dongliang
Li, Jianghua
Zhou, Guanyu
Zhang, Juan
Du, Guocheng
author_facet Wu, Xinying
Cai, Wen
Zhu, Pengcheng
Peng, Zheng
Zheng, Tianfei
Li, Dongliang
Li, Jianghua
Zhou, Guanyu
Zhang, Juan
Du, Guocheng
author_sort Wu, Xinying
collection PubMed
description Flue-cured tobacco (FCT) is an economical raw material whose quality affects the quality and cost of the derived product. However, the time-consuming and inefficient spontaneous aging is the primary process for improving the FCT quality in the industry. In this study, a function-driven co-culture with functional microorganisms was built in response to the quality-driven need for less irritation and more aroma in FCT. The previous study has found that Bacillus kochii SC could degrade starch and protein to reduce tobacco irritation and off-flavors. The Filobasidium magnum F7 with high lipoxygenase activity was screened out for degrading higher fatty acid esters and terpenoids to promote the aroma and flavor of FCT. Co-cultivation with strain SC and F7 obtained better quality improvement than mono-culture at an initial inoculation ratio of 1:3 for 2 days, representing a significant breakthrough in efficiency and a reduction in production costs compared to the more than 2 years required for the spontaneous aging process. Through the analysis of microbial diversity, predicted flora functions, enzyme activities and volatile compositions within the mono- and co-cultivation, our study showed the formation of a function-driven co-culture between two strains through functional division of labor and nutritional feeding. Herein, the function-driven co-culture via bioaugmentation will become an increasingly implemented approach for the tobacco industry.
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spelling pubmed-99783712023-03-03 Function-driven design of Bacillus kochii and Filobasidium magnum co-culture to improve quality of flue-cured tobacco Wu, Xinying Cai, Wen Zhu, Pengcheng Peng, Zheng Zheng, Tianfei Li, Dongliang Li, Jianghua Zhou, Guanyu Zhang, Juan Du, Guocheng Front Microbiol Microbiology Flue-cured tobacco (FCT) is an economical raw material whose quality affects the quality and cost of the derived product. However, the time-consuming and inefficient spontaneous aging is the primary process for improving the FCT quality in the industry. In this study, a function-driven co-culture with functional microorganisms was built in response to the quality-driven need for less irritation and more aroma in FCT. The previous study has found that Bacillus kochii SC could degrade starch and protein to reduce tobacco irritation and off-flavors. The Filobasidium magnum F7 with high lipoxygenase activity was screened out for degrading higher fatty acid esters and terpenoids to promote the aroma and flavor of FCT. Co-cultivation with strain SC and F7 obtained better quality improvement than mono-culture at an initial inoculation ratio of 1:3 for 2 days, representing a significant breakthrough in efficiency and a reduction in production costs compared to the more than 2 years required for the spontaneous aging process. Through the analysis of microbial diversity, predicted flora functions, enzyme activities and volatile compositions within the mono- and co-cultivation, our study showed the formation of a function-driven co-culture between two strains through functional division of labor and nutritional feeding. Herein, the function-driven co-culture via bioaugmentation will become an increasingly implemented approach for the tobacco industry. Frontiers Media S.A. 2023-02-16 /pmc/articles/PMC9978371/ /pubmed/36875537 http://dx.doi.org/10.3389/fmicb.2022.1024005 Text en Copyright © 2023 Wu, Cai, Zhu, Peng, Zheng, Li, Li, Zhou, Zhang and Du. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wu, Xinying
Cai, Wen
Zhu, Pengcheng
Peng, Zheng
Zheng, Tianfei
Li, Dongliang
Li, Jianghua
Zhou, Guanyu
Zhang, Juan
Du, Guocheng
Function-driven design of Bacillus kochii and Filobasidium magnum co-culture to improve quality of flue-cured tobacco
title Function-driven design of Bacillus kochii and Filobasidium magnum co-culture to improve quality of flue-cured tobacco
title_full Function-driven design of Bacillus kochii and Filobasidium magnum co-culture to improve quality of flue-cured tobacco
title_fullStr Function-driven design of Bacillus kochii and Filobasidium magnum co-culture to improve quality of flue-cured tobacco
title_full_unstemmed Function-driven design of Bacillus kochii and Filobasidium magnum co-culture to improve quality of flue-cured tobacco
title_short Function-driven design of Bacillus kochii and Filobasidium magnum co-culture to improve quality of flue-cured tobacco
title_sort function-driven design of bacillus kochii and filobasidium magnum co-culture to improve quality of flue-cured tobacco
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978371/
https://www.ncbi.nlm.nih.gov/pubmed/36875537
http://dx.doi.org/10.3389/fmicb.2022.1024005
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