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Evaluation of Sampling and Concentration Methods for Salmonella enterica Serovar Typhi Detection from Wastewater
Salmonella enterica serovar (Salmonella Typhi) is the causative bacterial agent of typhoid fever. Environmental surveillance of wastewater and wastewater-impacted surface waters has proven effective in monitoring various pathogens and has recently been applied to Salmonella Typhi. This study evaluat...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The American Society of Tropical Medicine and Hygiene
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978546/ https://www.ncbi.nlm.nih.gov/pubmed/36746655 http://dx.doi.org/10.4269/ajtmh.22-0427 |
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author | Zhou, Nicolette Ong, Angelo Fagnant-Sperati, Christine Harrison, Joanna Kossik, Alexandra Beck, Nicola Shirai, Jeffry Burnor, Elisabeth Swanstrom, Rachael Demeke, Bethel Patel, Suhani Scott Meschke, John |
author_facet | Zhou, Nicolette Ong, Angelo Fagnant-Sperati, Christine Harrison, Joanna Kossik, Alexandra Beck, Nicola Shirai, Jeffry Burnor, Elisabeth Swanstrom, Rachael Demeke, Bethel Patel, Suhani Scott Meschke, John |
author_sort | Zhou, Nicolette |
collection | PubMed |
description | Salmonella enterica serovar (Salmonella Typhi) is the causative bacterial agent of typhoid fever. Environmental surveillance of wastewater and wastewater-impacted surface waters has proven effective in monitoring various pathogens and has recently been applied to Salmonella Typhi. This study evaluated eight sample collection and concentration methods with 12 variations currently being developed and used for Salmonella Typhi surveillance globally to better understand the performance of each method based on its ability to detect Salmonella Typhi and its feasibility. Salmonella Typhi strains Ty21a and Ty2 were seeded to influent wastewater at known concentrations to evaluate the following methods: grab sampling using electropositive filters, centrifugation, direct enrichment, or membrane filtration and trap sampling using Moore swabs. Concentrated samples underwent nucleic acid extraction and were detected and/or quantified via quantitative polymerase chain reaction (qPCR). Results suggest that all methods tested can be successful at concentrating Salmonella Typhi for subsequent detection by qPCR, although each method has its own strengths and weaknesses, including the Salmonella Typhi concentration it is best suited for, with a range of positive detections observed as low as 0.1–0.001 colony-forming units (CFU) Ty21a/mL and 0.01 CFU Ty2/mL. These factors should be considered when identifying a method for environmental surveillance and will greatly depend on the use case planned. |
format | Online Article Text |
id | pubmed-9978546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The American Society of Tropical Medicine and Hygiene |
record_format | MEDLINE/PubMed |
spelling | pubmed-99785462023-03-03 Evaluation of Sampling and Concentration Methods for Salmonella enterica Serovar Typhi Detection from Wastewater Zhou, Nicolette Ong, Angelo Fagnant-Sperati, Christine Harrison, Joanna Kossik, Alexandra Beck, Nicola Shirai, Jeffry Burnor, Elisabeth Swanstrom, Rachael Demeke, Bethel Patel, Suhani Scott Meschke, John Am J Trop Med Hyg Research Article Salmonella enterica serovar (Salmonella Typhi) is the causative bacterial agent of typhoid fever. Environmental surveillance of wastewater and wastewater-impacted surface waters has proven effective in monitoring various pathogens and has recently been applied to Salmonella Typhi. This study evaluated eight sample collection and concentration methods with 12 variations currently being developed and used for Salmonella Typhi surveillance globally to better understand the performance of each method based on its ability to detect Salmonella Typhi and its feasibility. Salmonella Typhi strains Ty21a and Ty2 were seeded to influent wastewater at known concentrations to evaluate the following methods: grab sampling using electropositive filters, centrifugation, direct enrichment, or membrane filtration and trap sampling using Moore swabs. Concentrated samples underwent nucleic acid extraction and were detected and/or quantified via quantitative polymerase chain reaction (qPCR). Results suggest that all methods tested can be successful at concentrating Salmonella Typhi for subsequent detection by qPCR, although each method has its own strengths and weaknesses, including the Salmonella Typhi concentration it is best suited for, with a range of positive detections observed as low as 0.1–0.001 colony-forming units (CFU) Ty21a/mL and 0.01 CFU Ty2/mL. These factors should be considered when identifying a method for environmental surveillance and will greatly depend on the use case planned. The American Society of Tropical Medicine and Hygiene 2023-03 2023-02-06 /pmc/articles/PMC9978546/ /pubmed/36746655 http://dx.doi.org/10.4269/ajtmh.22-0427 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhou, Nicolette Ong, Angelo Fagnant-Sperati, Christine Harrison, Joanna Kossik, Alexandra Beck, Nicola Shirai, Jeffry Burnor, Elisabeth Swanstrom, Rachael Demeke, Bethel Patel, Suhani Scott Meschke, John Evaluation of Sampling and Concentration Methods for Salmonella enterica Serovar Typhi Detection from Wastewater |
title | Evaluation of Sampling and Concentration Methods for Salmonella enterica Serovar Typhi Detection from Wastewater |
title_full | Evaluation of Sampling and Concentration Methods for Salmonella enterica Serovar Typhi Detection from Wastewater |
title_fullStr | Evaluation of Sampling and Concentration Methods for Salmonella enterica Serovar Typhi Detection from Wastewater |
title_full_unstemmed | Evaluation of Sampling and Concentration Methods for Salmonella enterica Serovar Typhi Detection from Wastewater |
title_short | Evaluation of Sampling and Concentration Methods for Salmonella enterica Serovar Typhi Detection from Wastewater |
title_sort | evaluation of sampling and concentration methods for salmonella enterica serovar typhi detection from wastewater |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978546/ https://www.ncbi.nlm.nih.gov/pubmed/36746655 http://dx.doi.org/10.4269/ajtmh.22-0427 |
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