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Characterization of a lytic Escherichia coli phage CE1 and its potential use in therapy against avian pathogenic Escherichia coli infections

The high incidence of Avian pathogenic Escherichia coli (APEC) in poultry has resulted in significant economic losses. It has become necessary to find alternatives to antibiotics due to the alarming rise in antibiotic resistance. Phage therapy has shown promising results in numerous studies. In the...

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Autores principales: Tang, Zhaohui, Tang, Ning, Wang, Xinwei, Ren, Huiying, Zhang, Can, Zou, Ling, Han, Lei, Guo, Longzong, Liu, Wenhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978775/
https://www.ncbi.nlm.nih.gov/pubmed/36876110
http://dx.doi.org/10.3389/fmicb.2023.1091442
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author Tang, Zhaohui
Tang, Ning
Wang, Xinwei
Ren, Huiying
Zhang, Can
Zou, Ling
Han, Lei
Guo, Longzong
Liu, Wenhua
author_facet Tang, Zhaohui
Tang, Ning
Wang, Xinwei
Ren, Huiying
Zhang, Can
Zou, Ling
Han, Lei
Guo, Longzong
Liu, Wenhua
author_sort Tang, Zhaohui
collection PubMed
description The high incidence of Avian pathogenic Escherichia coli (APEC) in poultry has resulted in significant economic losses. It has become necessary to find alternatives to antibiotics due to the alarming rise in antibiotic resistance. Phage therapy has shown promising results in numerous studies. In the current study, a lytic phage vB_EcoM_CE1 (short for CE1) against Escherichia coli (E. coli) was isolated from broiler feces, showing a relatively wide host range and lysing 56.9% (33/58) of high pathogenic strains of APEC. According to morphological observations and phylogenetic analysis, phage CE1 belongs to the Tequatrovirus genus, Straboviridae family, containing an icosahedral capsid (80 ~ 100 nm in diameter) and a retractable tail (120 nm in length). This phage was stable below 60°C for 1 h over the pH range of 4 to 10. Whole-genome sequencing revealed that phage CE1 contained a linear double-stranded DNA genome spanning 167,955 bp with a GC content of 35.4%. A total of 271 ORFs and 8 tRNAs were identified. There was no evidence of virulence genes, drug-resistance genes, or lysogeny genes in the genome. The in vitro test showed high bactericidal activity of phage CE1 against E. coli at a wide range of MOIs, and good air and water disinfectant properties. Phage CE1 showed perfect protection against broilers challenged with APEC strain in vivo. This study provides some basic information for further research into treating colibacillosis, or killing E. coli in breeding environments.
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spelling pubmed-99787752023-03-03 Characterization of a lytic Escherichia coli phage CE1 and its potential use in therapy against avian pathogenic Escherichia coli infections Tang, Zhaohui Tang, Ning Wang, Xinwei Ren, Huiying Zhang, Can Zou, Ling Han, Lei Guo, Longzong Liu, Wenhua Front Microbiol Microbiology The high incidence of Avian pathogenic Escherichia coli (APEC) in poultry has resulted in significant economic losses. It has become necessary to find alternatives to antibiotics due to the alarming rise in antibiotic resistance. Phage therapy has shown promising results in numerous studies. In the current study, a lytic phage vB_EcoM_CE1 (short for CE1) against Escherichia coli (E. coli) was isolated from broiler feces, showing a relatively wide host range and lysing 56.9% (33/58) of high pathogenic strains of APEC. According to morphological observations and phylogenetic analysis, phage CE1 belongs to the Tequatrovirus genus, Straboviridae family, containing an icosahedral capsid (80 ~ 100 nm in diameter) and a retractable tail (120 nm in length). This phage was stable below 60°C for 1 h over the pH range of 4 to 10. Whole-genome sequencing revealed that phage CE1 contained a linear double-stranded DNA genome spanning 167,955 bp with a GC content of 35.4%. A total of 271 ORFs and 8 tRNAs were identified. There was no evidence of virulence genes, drug-resistance genes, or lysogeny genes in the genome. The in vitro test showed high bactericidal activity of phage CE1 against E. coli at a wide range of MOIs, and good air and water disinfectant properties. Phage CE1 showed perfect protection against broilers challenged with APEC strain in vivo. This study provides some basic information for further research into treating colibacillosis, or killing E. coli in breeding environments. Frontiers Media S.A. 2023-02-16 /pmc/articles/PMC9978775/ /pubmed/36876110 http://dx.doi.org/10.3389/fmicb.2023.1091442 Text en Copyright © 2023 Tang, Tang, Wang, Ren, Zhang, Zou, Han, Guo and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tang, Zhaohui
Tang, Ning
Wang, Xinwei
Ren, Huiying
Zhang, Can
Zou, Ling
Han, Lei
Guo, Longzong
Liu, Wenhua
Characterization of a lytic Escherichia coli phage CE1 and its potential use in therapy against avian pathogenic Escherichia coli infections
title Characterization of a lytic Escherichia coli phage CE1 and its potential use in therapy against avian pathogenic Escherichia coli infections
title_full Characterization of a lytic Escherichia coli phage CE1 and its potential use in therapy against avian pathogenic Escherichia coli infections
title_fullStr Characterization of a lytic Escherichia coli phage CE1 and its potential use in therapy against avian pathogenic Escherichia coli infections
title_full_unstemmed Characterization of a lytic Escherichia coli phage CE1 and its potential use in therapy against avian pathogenic Escherichia coli infections
title_short Characterization of a lytic Escherichia coli phage CE1 and its potential use in therapy against avian pathogenic Escherichia coli infections
title_sort characterization of a lytic escherichia coli phage ce1 and its potential use in therapy against avian pathogenic escherichia coli infections
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978775/
https://www.ncbi.nlm.nih.gov/pubmed/36876110
http://dx.doi.org/10.3389/fmicb.2023.1091442
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