Cargando…
Lymphoid Lineage γδ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium
γδ T cells are a rare and unique prototype of T cells that share properties with natural killer cells in secondary lymphoid organs. Although many studies have revealed the function and importance of adult-derived γδ T cells in cancer biology and regenerative medicine, the low numbers of these cells...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Stem Cell Research
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978832/ https://www.ncbi.nlm.nih.gov/pubmed/36310028 http://dx.doi.org/10.15283/ijsc22150 |
_version_ | 1784899605706571776 |
---|---|
author | Jeon, Soo-Been Han, A-Reum Choi, Yoo Bin Lee, Ah Reum Lee, Ji Yoon |
author_facet | Jeon, Soo-Been Han, A-Reum Choi, Yoo Bin Lee, Ah Reum Lee, Ji Yoon |
author_sort | Jeon, Soo-Been |
collection | PubMed |
description | γδ T cells are a rare and unique prototype of T cells that share properties with natural killer cells in secondary lymphoid organs. Although many studies have revealed the function and importance of adult-derived γδ T cells in cancer biology and regenerative medicine, the low numbers of these cells hamper their application as therapeutic cell sources in the clinic. To solve this problem, pluripotent stem cell-derived γδ T cells are considered alternative cell sources; however, few studies have reported the generation of human pluripotent stem cell-derived γδ T cells. In the present study, we investigated whether lymphoid lineage γδ T cells were successfully generated from human pluripotent stem cells via hemogenic endothelium under defined culture conditions. Our results revealed that pluripotent stem cells successfully generated γδ T cells with an overall increase in transcriptional activity of lymphoid lineage genes and cytolytic factors, indicating the importance of the optimization of culture conditions in generating lymphoid lineage γδ T cells. We uncovered an initial step in differentiating γδ T cells that could be applied to basic and translational investigations in the field of cancer biology. Based on our result, we will develop an appropriate method to purify γδ T cells with functionality and it helpful for the study of basic mechanism of γδ T cells in pathophysiologic condition as well as clinic application. |
format | Online Article Text |
id | pubmed-9978832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Korean Society for Stem Cell Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-99788322023-03-03 Lymphoid Lineage γδ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium Jeon, Soo-Been Han, A-Reum Choi, Yoo Bin Lee, Ah Reum Lee, Ji Yoon Int J Stem Cells Brief Report γδ T cells are a rare and unique prototype of T cells that share properties with natural killer cells in secondary lymphoid organs. Although many studies have revealed the function and importance of adult-derived γδ T cells in cancer biology and regenerative medicine, the low numbers of these cells hamper their application as therapeutic cell sources in the clinic. To solve this problem, pluripotent stem cell-derived γδ T cells are considered alternative cell sources; however, few studies have reported the generation of human pluripotent stem cell-derived γδ T cells. In the present study, we investigated whether lymphoid lineage γδ T cells were successfully generated from human pluripotent stem cells via hemogenic endothelium under defined culture conditions. Our results revealed that pluripotent stem cells successfully generated γδ T cells with an overall increase in transcriptional activity of lymphoid lineage genes and cytolytic factors, indicating the importance of the optimization of culture conditions in generating lymphoid lineage γδ T cells. We uncovered an initial step in differentiating γδ T cells that could be applied to basic and translational investigations in the field of cancer biology. Based on our result, we will develop an appropriate method to purify γδ T cells with functionality and it helpful for the study of basic mechanism of γδ T cells in pathophysiologic condition as well as clinic application. Korean Society for Stem Cell Research 2022-10-31 /pmc/articles/PMC9978832/ /pubmed/36310028 http://dx.doi.org/10.15283/ijsc22150 Text en Copyright © 2023 by the Korean Society for Stem Cell Research https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Report Jeon, Soo-Been Han, A-Reum Choi, Yoo Bin Lee, Ah Reum Lee, Ji Yoon Lymphoid Lineage γδ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium |
title | Lymphoid Lineage γδ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium |
title_full | Lymphoid Lineage γδ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium |
title_fullStr | Lymphoid Lineage γδ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium |
title_full_unstemmed | Lymphoid Lineage γδ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium |
title_short | Lymphoid Lineage γδ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium |
title_sort | lymphoid lineage γδ t cells were successfully generated from human pluripotent stem cells via hemogenic endothelium |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978832/ https://www.ncbi.nlm.nih.gov/pubmed/36310028 http://dx.doi.org/10.15283/ijsc22150 |
work_keys_str_mv | AT jeonsoobeen lymphoidlineagegdtcellsweresuccessfullygeneratedfromhumanpluripotentstemcellsviahemogenicendothelium AT hanareum lymphoidlineagegdtcellsweresuccessfullygeneratedfromhumanpluripotentstemcellsviahemogenicendothelium AT choiyoobin lymphoidlineagegdtcellsweresuccessfullygeneratedfromhumanpluripotentstemcellsviahemogenicendothelium AT leeahreum lymphoidlineagegdtcellsweresuccessfullygeneratedfromhumanpluripotentstemcellsviahemogenicendothelium AT leejiyoon lymphoidlineagegdtcellsweresuccessfullygeneratedfromhumanpluripotentstemcellsviahemogenicendothelium |