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Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility
BACKGROUND: Comparative and comprehensive omics studies have recently been conducted to provide a comprehensive understanding of the biological mechanisms underlying infertility. However, because these huge omics datasets often contain irrelevant information, editing strategies for summarizing and f...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9979430/ https://www.ncbi.nlm.nih.gov/pubmed/36859388 http://dx.doi.org/10.1186/s40104-023-00836-1 |
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author | Park, Yoo-Jin Pang, Won-Ki Pang, Myung-Geol |
author_facet | Park, Yoo-Jin Pang, Won-Ki Pang, Myung-Geol |
author_sort | Park, Yoo-Jin |
collection | PubMed |
description | BACKGROUND: Comparative and comprehensive omics studies have recently been conducted to provide a comprehensive understanding of the biological mechanisms underlying infertility. However, because these huge omics datasets often contain irrelevant information, editing strategies for summarizing and filtering the data are necessary prerequisite steps for identifying biomarkers of male fertility. Here, we attempted to integrate omics data from spermatozoa with normal and below-normal fertility from boars and bulls, including transcriptomic, proteomic, and metabolomic data. Pathway enrichment analysis was conducted and visualized using g:Profiler, Cytoscape, EnrichmentMap, and AutoAnnotation to determine fertility-related biological functions according to species. RESULTS: In particular, gamete production and protein biogenesis-associated pathways were enriched in bull spermatozoa with below-normal fertility, whereas mitochondrial-associated metabolic pathways were enriched in boar spermatozoa with normal fertility. These results indicate that below-normal fertility may be determined by aberrant regulation of protein synthesis during spermatogenesis, and the modulation of reactive oxygen species generation to maintain capacitation and the acrosome reaction governs boar sperm fertility. CONCLUSION: Overall, this approach demonstrated that distinct molecular pathways drive sperm fertility in mammals in a species-dependent manner. Moreover, we anticipate that searching for species-specific signaling pathways may aid in the discovery of fertility-related biomarkers within large omics datasets. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-023-00836-1. |
format | Online Article Text |
id | pubmed-9979430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-99794302023-03-03 Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility Park, Yoo-Jin Pang, Won-Ki Pang, Myung-Geol J Anim Sci Biotechnol Research BACKGROUND: Comparative and comprehensive omics studies have recently been conducted to provide a comprehensive understanding of the biological mechanisms underlying infertility. However, because these huge omics datasets often contain irrelevant information, editing strategies for summarizing and filtering the data are necessary prerequisite steps for identifying biomarkers of male fertility. Here, we attempted to integrate omics data from spermatozoa with normal and below-normal fertility from boars and bulls, including transcriptomic, proteomic, and metabolomic data. Pathway enrichment analysis was conducted and visualized using g:Profiler, Cytoscape, EnrichmentMap, and AutoAnnotation to determine fertility-related biological functions according to species. RESULTS: In particular, gamete production and protein biogenesis-associated pathways were enriched in bull spermatozoa with below-normal fertility, whereas mitochondrial-associated metabolic pathways were enriched in boar spermatozoa with normal fertility. These results indicate that below-normal fertility may be determined by aberrant regulation of protein synthesis during spermatogenesis, and the modulation of reactive oxygen species generation to maintain capacitation and the acrosome reaction governs boar sperm fertility. CONCLUSION: Overall, this approach demonstrated that distinct molecular pathways drive sperm fertility in mammals in a species-dependent manner. Moreover, we anticipate that searching for species-specific signaling pathways may aid in the discovery of fertility-related biomarkers within large omics datasets. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-023-00836-1. BioMed Central 2023-03-02 /pmc/articles/PMC9979430/ /pubmed/36859388 http://dx.doi.org/10.1186/s40104-023-00836-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Park, Yoo-Jin Pang, Won-Ki Pang, Myung-Geol Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility |
title | Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility |
title_full | Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility |
title_fullStr | Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility |
title_full_unstemmed | Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility |
title_short | Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility |
title_sort | integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9979430/ https://www.ncbi.nlm.nih.gov/pubmed/36859388 http://dx.doi.org/10.1186/s40104-023-00836-1 |
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